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Molecular mechanisms of oviposition control

Research Project

Project/Area Number 04044079
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionNagoya University

Principal Investigator

SHIMADA Kiyoshi  Nagoya University, professor, 農学部, 教授 (40065579)

Co-Investigator(Kenkyū-buntansha) 蛭薙 観順  名古屋大学, 農学部, 助手 (00126898)
RZASA Janusz  ポーランド農学アカデミー, 教授
HERTELENDY F.  Univ. St.Louis School of Medicine, 医学部, 教授
CORNETT L.E.  Univ. Arkansas for Medical Sciences, 医学部, 教授
ETCHES R.J.  Univ. Guelph, 農学部, 教授
RZASA J.  Poland Academy of Agriculture
HIRUNAGI K.  Nagoya Univ.
FRANK Hertel  セントルイス大学, 医学部, 教授
TOMAS I Koik  アーカンソー大学, 医学部, 教授
ROBERT J Etc  ゲルフ大学, 農学部, 教授
斎藤 昇  名古屋大学, 農学部, 助手 (40211924)
田中 耕作  九州大学, 農学部, 教授 (50038220)
田中 克英  岐阜大学, 農学部, 教授 (20021678)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥9,200,000 (Direct Cost: ¥9,200,000)
Fiscal Year 1993: ¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 1992: ¥4,500,000 (Direct Cost: ¥4,500,000)
Keywordsoviposition / gene expression / prostaglandin / uterus / arginine vasotocin / ovarian theca / hypothalamus / arginine vasotocin receptor / arginine vasotocin mRNA / アルギニンバソトシレセプター / アルギニンバソトシンmRNA / 卵巣
Research Abstract

We have proposed a hypothesis for oviposition mechanism as follows ; ovarian prostaglandin is released into peripheral plasma to stimulating uterine contractions, which in turn, triggers AVT release from the neurohypophysis. The present project was conducted to prove the above hypothesis by providing following evidence on the molecular basis.
1. Prostaglandins were produced in the theca layers of the largest pre- and postovulatory follicles and tissue PG level was highest with concurrent increase in plasma PGF concentration immediately before oviposition. Ovarian-oviposition inducing factor was identified as PG by HPLC analysis. These results indicate that ovarian PG plays a pivotal role in the regulation of oviposition.
2. Plasma AVT concentration also increased at the time of oviposition. AVT was released in response to uterine contractions but this increase was suppressed by indomethacin treatment which delayed oviposition. Immunocytochemical studies revealed that AVT-containing neuro … More ns were detected at the preoptic region and superficial area of the third ventricle. AVT cDNA was cloned and its base sequence was determined. Using the 562-base cDNA as a probe Northern hybridization analysis detected about 700 bases of AVT mRNA in the hypothalamus. The mRNA levels increased 4-fold after 4-day of water deprivation. Analysis of in situ hybridization revealed AVT mRNA localized in the periventricular nuclei of the hypothalamus.
3. Finally, the project of cloning of AVT receptor cDNA is stil in progress but solubilized fraction of AVT receptor of the chicken uterus was purified, electophoresed and this fraction was isolated. At present, amino acid sequencing is under an investigation and will be used for primers in PCR cloning to isolate the gene. On the basis of amino acid sequences of homologous region of human oxytocin receptor cDNA and rat arginine vasoporessin receptor, primers were synthesized for RT-PCR of chicken uterus RNA. The 452-bases RT-PCR cDNA product was used as a probe for Northern hybridization of the chicken uterus. Hybridizable mRNA species of 5.6, 4.6 and 2.1 kd were identified as AVT receptor mRNA. Less

Report

(2 results)
  • 1993 Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Ohkubo,T.,Tanaka,M.,Nakashima,K.,Shimada,K.,Saito,N. and Sato,K.: "High-level expression of biologically active chicken prolactin in E.coli." Comp.Biochem.Physiol.105A. 123-128 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 佐々木健、齋藤昇、島田清司: "産卵鶏の放卵に伴うアルギニンバソトシン濃度の変動" 鳥類内分泌研究会. 22-23 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Uchiyama,M.,Saito,N.,Shimada,K. and Murakami,T.: "Pituitary and plasma arginine vasotocin levels in the lamprey,Lamperta japonica." Comp.Biochem.Physiol.107A. 23-26 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sechman,A.,Shimada,K.,Saito,N.,Ieda,T. and Ono,T.: "Tissue-specific expression of calbindin-D_<23K> gen during ontogeny of the chicken." J.Expt.2001. (in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 神作宜男、寺田修、齋藤昇、島田清司: "チャボの就巣に伴う下垂体ホルモン遺伝子発現" 東海畜産学会報. (in press). (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Chaturvedi,C.M.,Koike,T.I.,Cornett,L.E. and Shimada,K.: "Localization of vasotocin (AVT) mRNA in dehydrated chickens." Gen.Comp.Endocrinol.(in press). (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hirunagi,K.,Rommel,E.,Oksche,A. and Korf,H.-W.: "Vasoactive intestinal peptide-immunoreactive cerebrospinal fluid-contacting neuronsin the reptilian lateral・・・" Cell Tissue Res.274. 79-90 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Shimada,K.,Ohkubo,T.,Saito,N.,Talbot,R.T. and Sharp,P.J.: "Avian Endocrinology" J.Endocrinology Ltd., 13 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Saito,N.,Shimada,K. and Koike,T.I.: "Avian Endocrinology" J.Endocrinology Ltd., 8 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Korf,H.-W.,Rommel,E.,Hirunagi,K. and Oksche,A.: "Integrative and Cellular Aspects of Autonomic Functions:temperature- and osmoregulation" Libbey, 12 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ohkubo,T., Tanaka,M., Nakashima,K., Shimada,K., Saito,N. and Sato,K.: "High-level expression of biologically active chicken prolactin in E.coll." Comp.Biochem.Physiol.105A. 123-128 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sasaki,K., Saito,N. and Shimada,K.: "Changes in AVT concentrations in plasma, neurohypophysis and hypothalamus in relation to oviposition in the chicken." Eighteenth Annual Meeting of Japanese Avian Endocrinology. 22-23 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Uchiyama,M., Saito,N., Shimada,K. and Murakami,T.: "Pituitary and plasma arginine vasotocin levels in the lamprey, Lamperta japonica." Comp.Biochem.Physiol.107A. 23-26 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sechman,A., Shimada,K., Saito,N., Ieda,T. and Ono,T.: "Tissue-specific expression of calbindin-D_<28K> gene during ontogeny of the chicken." J.Expt.2001. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kansaku,N., Terada,O., Saito,N. and Shimada,K.: "Gene expression of pituitary hormones in relation to broodiness of Chabo hens." The Tokai Journal of Animal Production. (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Chaturvedi,C.M., Koike,T.I., Cornett,L.E. and Shimada,K.: "Localization of vasotocin (AVT) mRNA in dehydrated chickens." Gen.Comp.Endocrinol.(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hirunagi,K., Rommel,E., Oksche,A. and Korf,H.-W.: "Vasoactive intestinal peptide-immunoreactive cerebrospinal fluid-contacting neurons in the reptilian lateral septum/nucleus accumbens." Cell Tissue Res.274. 79-90 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Shimada,K.: J. Endocrinology Ltd.Avian Endocrinology, 135-147 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Saito,N.: J. Endocrinology Ltd.Avian Endocrinology, 359-366 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Korf,H.-W., Rommel,E., Hirunagi,K. and Oksche,A.: Libbey. Integrative and Cellular Aspects of Autonomic Functions : temperature- and osmoregulation, 407-418 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary

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Published: 1992-04-01   Modified: 2016-04-21  

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