| Project/Area Number |
04044089
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Institution | Kyoto University |
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Principal Investigator |
SATOH Noriyuki Kyoto Univ., Faculty of Science, Assoc.Prof., 理学部, 助教授 (30025481)
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| Co-Investigator(Kenkyū-buntansha) |
JEFFERY William R. Bodega Marine Laboratory, Univ. of California USA, Professor, ボデガ海洋研究所, 教授
ウィリアム R ジェフェ 米国カリフォルニア大学, ボデガ海洋研究所, 教授
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 1993: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1992: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | Developmental pattern / Egg cytoplasmic factors / gene expression / Notochord / Muscle / Pigment cells / Prochordates / Evolution |
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| Research Abstract |
A recent molecular-phylogenetic study based on the comparison of 18S rDNA sequences has suggested that formation of tadpole larvae is a key developmental event to understand the origin and evolution of chordates. During embryogenesis of ascidians, about forty unicellular and striated muscle cells as well as forty notochord cells are formed in the tail of the larva. Therefore, it is important to study molecular mechanisms involved in differentiation of these cells to understand not only ontogeny by also phylogeny of animals.
We have isolated an ascidian homolog of the mouse T (Brachyury) gene. The ascidian T gene is expressed exclusively in notochord cells and the expression begins with the time of restriction of their developmental fates. Therefore, the T gene is cue to understand the chordate evolution. We are now studying the T gene expression in ascidians which develop tailless larvae.
Muscle cell differentiation in ascidian embryos has been studied at the levels of muscle actin genes, myogenic regulatory gene, and cytoplasmic factors (or muscle determinants). We have also studied muscle actin genes in ascidians with tailless larvae, and it was found that the ascidian with tailless larvae contains several pseudogenes of muscle actin, which were not found in ascidians with tailed larvae under usual research conditions we adopted.
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