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Molecular Biology of Lyme Borreliosis and Lyme Disease Control

Research Project

Project/Area Number 04044138
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionUniversity of Shizuoka

Principal Investigator

YANAGIHARA Yasutake  University of Shizuoka, Professor, 薬学部, 教授 (30046255)

Co-Investigator(Kenkyū-buntansha) JOHNSON Russ  ミネソタ大学, 医学部(米国), 教授
FUKUNAGA Masahito  Fukuyama University, Professor, 薬学部, 教授 (20132483)
MASUZAWA Toshiyuki  University of Shizuoka, Research Associate, 薬学部, 助手 (10181645)
IWAMOTO Yoshihisa  University of Shizuoka, Associate Professor, 薬学部, 助教授 (60046290)
JOHNSON Russell C.  University of Minnesota, U.S.A., Professor
R C.Johnson  米国ミネソタ大学, 医学部, 教授
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1993: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1992: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsLyme disease / Lyme disease borreliae / PCR / Serodiagnosis / Antibiotics / Vaccine / Genediagnosis / Borrelia japonica / ライム菌 / FRLP / rRNA遺伝子 / 鞭毛蛋白遺伝子 / シュルツェマダニ / ヤマトマダニ
Research Abstract

Dr.Johnson was invited to Japan and he gave lectures on present status of Lyme borreliosis research at University of Shizuoka and National Institute of Health of Japan. Masuzawa was dispatched to Dr.Johnson's laboratory, WHO work-shop on Lyme borreliosis control at International Zoonoses Conference, WHO Head-quarters, University of Neuchatel and Pasteur Institute to discuss on Lyme disease and borreliae.
RFLP ribotyping of 23S rRNA gene of isolates from the world corresponded to 3 genospecies, Borrelia burgdorferi, B. garinii and B. afzelii, and were applicable to classification of genospecies of borreliae. Genomic Southern hybridization of borrelial isolates with 23S rRNA probes showed that borreliae originated from I. persulcatus were classified into B. garinii, B. afzelii and others.
Borrelia isolated from I. ovatus in Japan was named B. japonica as new species based on morphology, DNA homology, G+C content, RFLP analysis of 16S rRNA genes and reactivity to monoclonal antibodies. Mono … More clonal antibodies capable of classifying B. japonica and other borreliae were prepared.
RFLP analysis using 16S rRNA gene probe BBU30 realized classification and identification of 4 genospecies and revealed that borreliae isolated from I. persulcatus and Apodemus speciosus ainu were belonging to B. garinii.
By PCR targetting flagellin gene, specifically amplified DNAs were detected from culture and experimentally infected ticks and mice. Two steps PCR detected 1 Borrelia cell and is applicable to gene diagnosis and epidemiological study.
PCR for Osp A and Osp B and RFLP analysis of the PCR products were useful for direct identification of 4 genospecies from clinical samples.
Specific lesion was induced by inoculation of pathogenic borreliae into footpad of outbred ddY mice. This animal model was useful in research for developingvaccine. Cross protection was not found among strains having different antigens of Osp A and B. Furthermore, Borrelia strains corresponding to endemic area are necessary for vaccine. The PCR developed in this study are useful for rapid identification of endemic strain. Less

Report

(2 results)
  • 1993 Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (38 results)

All Other

All Publications (38 results)

  • [Publications] Toshiyuki Masuzawa: "Experimental Borrelia burgdorferi infection of outbred mice" Journal of Clinical Microbiology. 30. 3016-3018 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masahito Fukunaga: "The 23S/5S ribosomal RNA genes(rrl/rrf)are separated from the 16S ribosomal RNA gene(rrs)in Borrelia burgdorferi,the aetiological agent of Lyme disease" Journal of General Microbiology. 138. 871-877 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Toshiyuki Masuzawa: "Protective activity of antisera against isolates of Borrelia burgdorferi from various geographical origins" Microbiology and Immunology. 37. 79-83 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masahito Fukunaga: "Analysis of Borrelia species associated with Lyme disease by rRNA gene restriction fragment length polymorphism" Journal of General Microbiology. 139. 1141-1146 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yukie Takahashi: "Characterization of Borrelia species isolated from ixodid ticks,Ixodes ovatus" Microbiology and Immunology. 37. 721-727 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masahito Fukunaga: "Antigenic and genetic characterization of Borrelia species isolated from Ixodes persulcatus in Hokkaido,Japan" Journal of Clinical Microbiology. 31. 1388-1391 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masahito Fukunaga: "Evaluation of genetic divergence of Borrelial isolates from Lyme disease patients in Hokkaido,by rRNA gene probes" Journal of Clinical Microbiology. 31. 2044-2048 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroki Kawabata: "Genomic analysis of Borrelia japonica sp.nov.isolated from Ixodes ovatus in Japan" Microbiology and Immunology. 37. 843-848 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Toshiyuki Masuzawa: "Experimental induction of Lyme arthritis in outbred mice" Microbiology and Immunology. 38(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Toshiyuki Masuzawa: "In vitro antibiotic susceptibilities of Borrelia isolates from erythema migrans lesion of Lyme disease patients in Japan" Microbiology and Immunology. 38(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Toshiyuki Masuzawa: "Characterization of monoclonal antibodies for identification of Borrelia japonica,isolated from Ixodes ovatus" Microbiology and Immunology. 38(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroki Kawabata: "Polymerase chain reaction analysis of Borrelia species isolated in Japan" Microbiology and Immunology. 38(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 柳原保武: "総説 ボレリア" 臨床と微生物. 19. 441-447 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 福長将仁: "総説 スピロヘータのリボソームRNA遺伝子の構成" 日本細菌学雑誌. 47. 679-687 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 増澤俊幸: "総説 ライム病ワクチン開発の動向" 日本細菌学雑誌. 48. 385-393 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 柳原保武: "戸田新細菌学(改訂第30版、森良一、天児和暢編)スピロヘータ分担" 南山堂, 987 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Toshiyuki Masuzawa: "Experimental Borrelia burgdorferi infection of outbred mice" Journal of Clinical Microbiology. 30. 3016-3018 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masahito Fukunaga: "The 23S/5S ribosomal RNA genes (rrl/rrf) are separated from the 16S ribosomal RNA gene (rrs) in Borrelia burgdorferi, the aetiological agent of Lyme disease" Journal of General Microbiology. 138. 871-877 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Toshiyuki Masuzawa: "Protective activity of antisera against isolates of Borrelia burgdorferi from various geographical origins" Microbiology and Immunology. 37. 79-83 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masahito Fukunaga: "Analysis of Borrelia species associated with Lyme disease by rRNA gene restriction fragment length polymorphism" Journal of General Microbiology. 139. 1141-1146 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yukie Takahashi: "Characterization of Borrelia species isolated from ixodid ticks, Ixodes ovatus" Microbiology and Immunology. 37. 721-727 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masahito Fukunaga: "Antigenic and genetic characterization of Borrelia species isolated from Ixodes persulcatus in Hokkaido, Japan" Journal of Clinical Microbiology. 31. 1388-1391 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masahito Fukunaga: "Evaluation of genetic divergence of Borrelial isolates from Lyme disease patients in Hokkaido, by rRNA gene probes" Journal of Clinical Microbiology. 31. 2044-2048 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroki Kawabata: "Genomic analysis of Borrelia japonica sp. nov. isolated from Ixodes ovatus in Japan" Microbiology and Immunology. 37. 843-848 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Toshiyuki Masuzawa: "Experimental induction of Lyme arthritis in outbred mice" Microbiology and Immunology. 38(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Toshiyuki Masuzawa: "In yitro antibiotic susceptibilities of Borrelia isolates from erythema migrans lesion of Lyme disease patients in Japan" Microbiology and Immunology. 38(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Toshiyuki Masuzawa: "Characterization of monoclonal antibodies for identification of Borrelia japonica, isolated from Ixodes ovatus" Microbiology and Immunology. 38(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroki Kawabata: "Polymerase chain reaction analysis of Borrelia species isolated in Japan" Microbiology and Immunology. 38(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yasutake Yanagihara: "A review : Borrelia (in Japanese)" Clinical Microbiology. 19. 441-447 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masahito Fukunaga: "A review : Organization of ribosomal RNA genes of spirochetes (in Japanese)" Japanese Journal of Bacteriology. 47. 679-687 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Toshiyuki Masuzawa: "A review : Current status of vaccine development of Lyme borreliosis (in Japanese)" Japanese Journal of Bacteriology. 48. 385-393 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yasutake Yanagihara: Nanzando Co.Ltd., Tokyo. Spirochetes in Toda's New Bacteriology, 30th Ed.(in Japanese), 520-537 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masahito Fukunaga: "The 23S/5S ribosomal RNA genes are separate from the 16S ribosomal RNA gene in Borre ia burgdorferi, the etiological agent of Lyme disease" Journal of General Microbiology. 138. 871-877 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hiroshi Isogai: "Seroepidemiological survey for antibody to Borrelia burgdorferi in cows" Microbiology and Immunology. 36. 1029-1039 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Toshiyuki Masuzawa: "Experimental Borrelia burgdorferi infection of outbred mice" Journal of Clinical Microbiology. 30. 3016-3018 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Toshiyuki Masuzawa: "Protective activity of aantisera against isolates of Borrelia burgdorferi from various geographical origins" Microbiology and Immunology. 37. 79-83 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Masahito Fukunaga: "Analysis of Borrelia species associated with Lyme disease by rRNA restriction gene fragment lengh polymorphism" Journal of General Microbiology. IN PRESS (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 増澤俊幸: "ライム菌ワクチン開発の動向" 日本細菌学雑誌. 48. 385-393 (1993)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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