A Basic Approach to Glycation as a Cause of Aging
| Project/Area Number |
04044200
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| Research Category |
Grant-in-Aid for Overseas Scientific Survey.
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| Allocation Type | Single-year Grants |
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ARAI Soich Faculty of Agriculture, The University of Tokyo, 農学部, 教授 (20011934)
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| Co-Investigator(Kenkyū-buntansha) |
梁 隆 延世大学校, 工科大学, 教授
FUJIMOTO Daisaburo Faculty of Agriculture, Tokyo University of Agriculture and Technology, 農学部, 教授 (40004288)
HAYASE Fumitaka Faculty of Agriculture, Meiji University, 農学部, 助教授 (80105246)
YANG Ryung College of Engineering, Yensei University
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| Project Period (FY) |
1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Keywords | Maillard reaction / Glycation / 3-Deoxyglucosone / 3-Deoxyfructose / Aldose reductase / 2-Oxoaldehyde reductase / Scavenger receptor / Imidazolone |
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| Research Abstract |
1.Comparison of reaction propagator activity to glycation.
When 3-deoxyglucosone (3DG) was incubated with serum of rats, most of 3Dg readily reacted with components of serum.
2.Chemical pathway of propagator formation.
^<14>C-Glucose was orally continuously administered to rats. Eight days after oral administration, ^<14>C-3-Deoxyfructose (3DF) which is a metabolite of 3DG, was detected by TLC-autoradiography.
3.Identification and distribution of reaction propagator in tissue.
Endogeneous 3DF was detected and identified in the urine of rats by oral administration of 3DG-free feed.
4.Reaction mechanism of propagator.
2-(delta-N-ornithy1)-5-{1-(2,3,4-trihydroxybuty1)}-4-imidazolone was identified in the reaction system of 3DG and N-alpha-benzoylarginine amide.
5.Self-defense mechanism against glycation.
Aldose reductase was purified and characterized from porcine liver as one of 3DG-metabolizing enzymes. 2-Oxoaldehyde reductase was detected in all chicken organs in which liver had highest activity, by using polyclonal antibody for 2-oxoaldehyde reductase purified from chicken liver. 3DG-modified BSA was recognized by the type I macrophage scavenger receptor expressed on COS cells.
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Report
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Research Products
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