造血幹細胞の分化増殖の分子生物学的解析

• Project/Area Number: 04247103
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Research Institution: Jichi Medical University
• Principal Investigator: 三浦 恭定 自治医科大学, 医学部, 教授 (60048965)
• Co-Investigator(Kenkyū-buntansha): 北村 幸彦 大阪大学, 医学部, 教授 (70028520) ; 穂積 本男 埼玉がんセンター研究所, 所長 (50113478) ; 新井 賢一 東京大学, 医科学研究所, 教授 (00012782) ; 溝口 秀明 (溝口 秀昭) 東京女子医科大学, 医学部, 教授 (70049021) ; 高久 文麿 (高久 史麿) 国立病院医療センサー, 院長 (40048955)
• Project Period (FY): 1992
• Project Status: Completed (Fiscal Year 1993)
• Budget Amount: ¥18,300,000 (Direct Cost: ¥18,300,000); Fiscal Year 1993: ¥3,000,000 (Direct Cost: ¥3,000,000); Fiscal Year 1992: ¥15,300,000 (Direct Cost: ¥15,300,000)
• Keywords: 造血幹細胞 / 造血因子 / 造血因子レセプター / 造血支持細胞 / 白血病細胞の分化 / C-Kit / 細胞内情報伝達 / 研究まとめ / c-kit / SCF / 造血器疾患 / フレンド白血病

Research Abstract

1)平成2年度より3年間にわたってきた重点領域研究「造血幹細胞の分化増殖の分子生物学的解析」の研究成果を取りまとめるために、研究に参加した全班員から報告の原稿を提出させた。研究代表者の総括報告等を加えて報告書を作成した。報告書はA4版、全体で300ページである。
2)成果とりまとめについて必要となった追加実験のために消費品を使用した。
3)成果報告やまとめの作業を補助するため、謝金を使用した。

Research Products

• [Publications] Gunji Y.: "Human Primitive hemopoietic progenitor cells are more enriched in KIT^<low1> cells than KIT^<high> cells." Blood. (in press).
• [Publications] Toyoshima H.: "Differently spliced cDNAS of human ltkreceptor tyrosine kinase domain and a soluble receptor protein." Proc.Natl.Acad.Sci.USA. 90. 5404-5408 (1993)
• [Publications] Tukada T.: "Enhanced proliferative potential in culture of cells from p53-deficient mice." Oncogene. 8. 3313-3322 (1993)
• [Publications] Hanazono Y.: "C-fps/fes protein-tyrosine kinase is implicated in signaling pathway triggered by granurocyte-macrophage colony-stimulating factor and interleukin-3." EMBO J.12. 1641-1646 (1993)
• [Publications] Kasukabe T.: "Characterization of growth inhibitory factors fo mouse monocytic leukemia cells." Leukemi Res.16. 139-144 (1992)
• [Publications] Kasugai T.: "lnfection of Nippostrongylus brasiliensis induced normal increade of basophils in mast cell-deficient Ws/Wsrats with a small deletion at the kinase domain of c-Kit." Blood. 81. 2521-2529 (993)
• [Publications] Arai K.: "IuPAC" New era of biochemistey cytokine signal network and its implication for biomedical research.Medical Chemistrv for 21th Century. 22 (1992)
• [Publications] Kitamura Y.: "Hogrete & Huber Publshers Publishers (inpress)" Deveiopment of mast cells and basophils.Udefulness of mutant mice and rats.in Progress in Allergy and Clinical lmmunology.
• [Publications] Gunji Y.: "Human primitive hemopoietic progenitor cells are more enriched in KIT ^<high>cells than KIT cells." Blood. 82. 3283-3289 (1993)
• [Publications] Toyoshima H.: "Differently spliced cDNAs of human RK receptor tyrosine kinase doinain and a soluble receptor protein." Proc.Natl.Acad.Sci.USA. 90. 5404-5408 (1993)
• [Publications] Tukada T.: "Enhanced proliferative potential in culture of cella from p53-deficient mice." Oncogene. 8. 3313-3322 (1993)
• [Publications] Hanazono Y.: "C-fos/fesprotein-tyrosine kinase is implicated in signaling pathway triggered by granulocyle-macrophage colony-stimulating lactor and interleukin-3." ENBO J.12. 1641-1646 (1993)
• [Publications] Kasukabe T.: "Characterization of growth inhibitory factors for mouse monocytic leukemia cells." Leuk.Res.16. 139-144 (1992)
• [Publications] Kasugai T.: "Infection of Nippostrongylus brasiliensis induced normal increase of basophils in mast cell-deficient Ws/Ws rats with a small deletion at the kinase domain of c-kit." Blood. 81. 2521-2529 (1993)
• [Publications] Arai K.: "New era of biochemistry:cytokine signal network and its implication for biomedical tesearch.Medical Chemistry for 21th Century" IUPAC, 22 (1992)
• [Publications] Kitamura Y.: "Development of mast cells and basophils.Usefulness of mutant mice and rats.In Progress in Allergy and Cinical lmmunology." Hogrete & Huber Publshers(in press),
• [Publications] Okada,S.,Suda,T.,Miura,Y.et al: "In vivo and in vitro stem cell function of c-kit and sca-l-positive murine hemopoietic cells." Blood. 80. 3304-3050 (1992)
• [Publications] Nakamura,Y.,Nakauchi,H.et al: "Dominant expression of the cytoplasmic tailless erythropoietin receptor is involved in programmed cell death of early erythroid progenitor cells." Science. 257. 1138 (1992)
• [Publications] Ebi,Y.,Kitamura,Y.et al: "Low c-kit expression of cultured mast cells of mi/mi genotype may be involved in their defective responses to fibroblast that express the ligand for c-kit." Blood. 80. 1454-1462 (1992)
• [Publications] Wang,H.M.,Arai,K-I.et al: "Structure of mouse IL-3 binding protein(AIC2A):Amino acid residues critical for IL-3 binding." J.Biol.Chem.267. 979-983 (1992)
• [Publications] Yamamura,Y.,Ikawa,Y.et al: "Distinct downstream signaling mechanism between erythropoietin receptor and interleukin-2 receptor." EMBO J.11. (1992)
• [Publications] Teramura,M.,Mizoguchi,H.et al: "Interleukin-11 enhances human megakaryocytopoiesis in vitro." Blood.79. 327-331 (1992)
• [Publications] Miura,Y.et al: "Differentiation pathways of clone-sorted CD34-positive cells.Myelodysplastic Syndrome & Cytokines," Elsevier Science Publishers B.V., 10 (1992)