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形質転換と大量集積

Research Project

Project/Area Number 04252102
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionNagoya University

Principal Investigator

町田 泰則  名古屋大学, 理学部, 教授 (80175596)

Co-Investigator(Kenkyū-buntansha) 佐藤 文彦  京都大学, 農学部, 助教授 (10127087)
中村 研三  名古屋大学, 農学部, 教授 (80164292)
内宮 博文  東京大学, 応用微生物研究所, 教授 (50142229)
岩渕 雅樹  京都大学, 理学部, 教授 (30000839)
Project Period (FY) 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥55,000,000 (Direct Cost: ¥55,000,000)
Fiscal Year 1992: ¥55,000,000 (Direct Cost: ¥55,000,000)
KeywordsTiプラスミドベクター / ヒストン遺伝子 / スポラシン遺伝子 / ショ糖誘導遺伝子 / rolC遺伝子 / シス領域 / ベルベリン合成 / ACPハイドロラーゼ
Research Abstract

本年度は、以下のような成果を挙げた。
(1)Agrobacteniumによる植物細胞の形質転換に関与している二つのタンパク質を同定し、それらの直接的な相互作用により、形質転換反応が誘発されることがわかった。また、タバコの根特異的プロモーターを用いることにより、スコポラミン生産能力が増大した形質転換ベラドンナの毛状根を作出した。その蓄積量はCaMV35Sプロモーターを用いた場合より、1.5倍高かった。(2)ヒストンH3遺伝子の細胞周期でのS期特異的発現は、二つのcis配列の協調的作用によることがわかった。(3)ショ糖に応答するrolCプロモーター領域に結合する一本鎖結合タンパク質を同定した。(4)ショ糖に応答するスポラミン遺伝子プロモーターには正と負のcis配列が存在することがわかった。(5)ベルベリン生合成の2つの0メチル化酵素のcDNAをクローン化した。(6)イオウ代謝サイクルに中心的な役割を果たしているシステイン合成酵素で細胞質と葉緑体に存在するものの遺伝子cDNAを高等植物からクローニングした。(7)エリシターによるイソフラボノイド生合成の発現とカルコン合成酵素遺伝子の大腸菌での発現を行った。(8)グルタミン合成酵素遺伝子の発現部位が、維管束細胞であることがわかった。(9)植物ウィルスRNA複製酵素遺伝子を発現している植物細胞を利用した物質生産系を確立した。(10)脂肪酸代謝の鍵酵素であるアシルACPヒドロラーゼの33kDaポリペプチドのアミノ末端の配列を34残基決定した。以上のようにこの3年間で、物質生産を担っている植物遺伝子のクローニングとその発現の分子機構の研究が進むと同時に、実際に遺伝子操作により高いレベルの物質生産系が確立しうるところまで進んできた。
以上の研究を遂行するために、計画通り設備備品を購入した。

Report

(1 results)
  • 1992 Annual Research Report
  • Research Products

    (40 results)

All Other

All Publications (40 results)

  • [Publications] Yasunori Machida: "Molecular interactions between Agrobacterium and plant cells in Molecualr Mechanisms of Bacterial Virulence (ed.by E.W.Nester)Kluwer" 85-96 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Takefumi Kawata: "A cDNA Clone encoding HBP-1b homologue in Arabidopsis thaliana" Nucl.Acids Res.20. 1141 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Takuya Nakayama: "Highly conserved hexamer,octamer and nonamer motifs are positive cis-regulatory elements of the wheat histone H3 gene" FEBS Lett.300. 167-170 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sumiko Sugaya: "Deletion analysis of the 5'-upstream region of the Agrobacterium rhizogenes Ri plasmid rolC gene required for tissue-specific expression" Plant Physiol.99. 464-467 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Akihiro Suzuki: "Signal-strand.DNA of 5'-upstream region of rolC gene interacts with unclear proteins of carrot cell culture" Biochem.&Biophys.Res.Comm.188. 727-733 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sumiko Katayama: "Analysis of intergenic spacer regions in the nuclear rDNA of Pharbitis nil" Genome. 35. 92-97 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Taku Takahashi: "Isolation and analysis of the expression of two genes for the 81-KD heat-shock proteins from Arabidopsis" Plant Physiol.99. 383-390 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sumie Ishiguro: "The nuclear factor SP8BF binds to the 5'-upstream regions of three different genes coding for major tuberous root protiens of sweet potato" Plant Mol.Biol.18. 97-108 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Masa-aki Ohto: "Induction of expression of genes coding for sporamin and β-amylase by polygalacturonic acid in leaf-petiole cutting of sweet potato" Plant Physiol.99. 422-427 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tomokazu Konishi: "Protein transport into thylakoid lumen-A stromal processing and energy requirement for the import of the precursor for the 23-kDa protein of PS II." Plant Cell Physiol.(1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 渡辺 昭: "緑葉の老化とグルタミン合成酵素" Nippon Nogeikagaku Kaishi. 66. 932-935 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Satomi Takeda: "Nucleotide sequence of a cDNA for Osmotin-like protein from cultured tobacco cells" Pleant Physiol.97. 844-846 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Fumihiko Sato: "S-adenosyl-L-methionine:scoulerine-9-0-methyltransferase from cultured Coptis japonica cells" Phytochem.(1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hyung-Hwang Park: "Isoflavone Glucosides Exist as Their 6¨-0-Malonyl Esters in Pueraria lobata and Its Cell Suspension Cultures" Chem.Pharm.Bull.40. 1978-1980 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Takashi Hakamatsuka: "Isoflavone Dimers from Yeast-Extract-Treated Cell Suspension Cultures of Pueraria lobata" Z.Naturforscheung. 47c. 177-182 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hiroyuki Takano: "Rapid clonal-propagation of Matricaria chamomilla by tissue-cultured shoot primordia" Japanese Jorunal of Breeding. 41. 421-426 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Toshifumi Hirata: "Lipid constituents oil bodies in shoot primordia of Matricaria chamomilla" Plant Cell Report. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Saito Kazuki: "Molecular cloning and bacterial expression of cDNA encoding a plant cysteine synthase" Proc.Natl.Acad.Sci.USA. 89. 8078-8082 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kazuki Saito: "Transgenic herbicide-resistant Atropa belladonna using an Ri binary vector and inheritance of the transgenic trait" Plant Cell Rep.11. 219-224 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yuji Minami: "Isolation and characterization of two momordins,ribosome-inactivating protiens form the seeds of bitter gourd (Momordica charantia)" Biosci.Biotech.Biochem.56. 1470-1471 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Masachika Hrie: "Base specificity of two plant seed ribonucleases from Momordica charantia and Luffa cylindrica" Biosci.Biotech.Biochem.57. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hiroyuki Imai: "Acyl-(acyl-carrier protein)hydrolase from squash cotyledons specific to long-chain fatty acids:purification and characterization" Plant Mol.Biol.20. 199-206 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ikuo Nishida: "The gene and the RNA for the precursor to the plastidlocated glycerol-3-phosphate acyltransferase of Arabidopsis thaliana" Plant Mol.Biol.21. 262-272 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kaoru Suzuki: "Effects of 2,6-dichlorobenzonitrile on differentiation to tracheary elements of isolated mesophylle cells of Zinnia elegans and formation of secondary cell walls" Physiol.Plant. 86. 43-48 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Masashi Mori: "Infectivity of plasmids containing brome mosaic virus cDNA linked to the cauliflower mosaic virus 35S RNA promoter" j.Gen.Virology. 72. 243-246 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Masahi Mori: "Expression of brome mosaic virus-encoded replicase genes in transgenic tobacco plants" J.Gen.Virology. 73. 169-172 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tsutomu Furuya: "Biotransformation of Tabersonine in Cell Suspension Cultures of Catharunthus roseus" Phytochemistry. 31. 3065-3069 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Takafumi Yoshikawa: "Continuous Production of Glycosides by Bioreactor Using Ginseng Hairy Root Cultures" Microbiology and Biothechnology. 38. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Koji Mikami: "Regulation of cell cycle-dependent gene expression.In"Control of Plant Gene Expression"(D.P.S.Verma,ed.)" CRC Press,Inc., 18 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Takuya Nakayama: "Regulation of wheat histone gene expression.In"Critical Reviews in Plant Science"(B.V.Conger,ed.)" CRC Press,Inc., (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hirofumi Uchimiya: "Production of transgenic rice plants by direct gene transfer into protoplasts.In Plant Transformation protocols(ed.L.Fitzmaurice and F.Lin)" Academic Press, (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yutaka Oono: "Molecular and cellular analysis of the rol genes of Agrobacterium rhizogenes Ri Plasmid." J.of Plant Research, (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 米田 好文: "「植物の分子生物学」シロイヌナズナの核ゲノム構成" 共立出版, 372 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 米田 好文: "「開花・結実の分子機構」花芽形成開始機構の分子遺伝学的研究" 秀潤社, 290 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 中村 研三: "「植物の分子生物学」糖による植物遺伝子の発現制御" 共立出版, 8 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 中村 研三: "植物におけるショ糖による遺伝子発現制御" 生化学, 10 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 谷口 研至: "苗条原基誘導法「最新バイオテクノロジー全書2 野菜の組織・細胞培養と増殖」" 秀潤社, 8 (1990)

    • Related Report
      1992 Annual Research Report
  • [Publications] 谷口 研至: "生長点培養 現代化学増刊「植物バイオテクノジーII」" 東京化学同人, 11 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ikuo Nishida: "Current Topics in Plant Physiology Series" American Society of Plant Physiology, (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 古谷 力: "Maruzen Advanced Technology 植物工学" 丸善株式会社, 260 (1993)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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