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ゲノムの複製終結と分配制御

Research Project

Project/Area Number 04256103
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionKumamoto University

Principal Investigator

平賀 壮太 (平賀 壯太)  熊本大学, 医学部, 教授 (40027321)

Co-Investigator(Kenkyū-buntansha) 小椋 光  熊本大学, 医学部, 助教授 (00158825)
西村 昭子  国立遺伝学研究所, 助教授 (20142002)
加藤 潤一  東京大学, 医科学研究所, 助手 (10194820)
永井 和夫  東京工業大学, 生命理工学部, 教授 (00011974)
岡崎 恒子  名古屋大学, 理学部, 教授 (10022584)
丹羽 修身  京都大学, 理学部, 助教授 (70144318)
Project Period (FY) 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥166,000,000 (Direct Cost: ¥166,000,000)
Fiscal Year 1994: ¥64,000,000 (Direct Cost: ¥64,000,000)
Fiscal Year 1993: ¥61,000,000 (Direct Cost: ¥61,000,000)
Fiscal Year 1992: ¥41,000,000 (Direct Cost: ¥41,000,000)
KeywordsMukB蛋白 / トポイソメラーゼ / FtsH蛋白 / ヒト染色体 / セントロメア / アルフォイドDNA / ミニ染色体 / 熱ショック転写因子 / 分裂酵母
Research Abstract

1.大腸菌染色体分配に関与するmukB遺伝子の上流に存在するofr50の破壊株を解析して、この遺伝子(mukFと命名)もmkB遺伝子と同じく染色体分配に関与することを明らかにした(平賀)。
2.ヒト染色体セントロメア領域に存在するアルフォイドDNA中のCEN-B-BoxとCEN-B蛋白の複合体の高次構造について解析した(岡崎)。
3.トポイソメラーゼIIαとIIβの両方の活性阻害をする抗体を注入すると細胞は中記まで進がそれ以後は進まないことを明らかにした(菊池)。
4.大腸菌のトポイソメラーゼIV(parCとparE)と相互作用をする蛋白質を検索することを目標として、dominant negativeなparC変異を抑制する多重コピー抑制遺伝子を多数分離し、解析した(加藤)。
5.FtsH蛋白は熱ショック転写因子δ32を分解するATP、Zn++依存性の新しいタイプの蛋白分解酵素であることを明らかにした(小椋)。
6.DNA合成阻害時においても細胞分裂の阻害が起こりにくいcfcA、cfcB変異がそれぞれglyS、apaHの変異であることを明らかにし、細胞内のAppppAの量が細胞分裂の頻度を調節していることを明らかにした(西村昭子)。
7.Fプラスミドの分配遺伝子sopBの欠失が宿主細胞の増殖を阻害する現象を解析して、この増殖阻害現象はsopB欠失のミニFプラスミドでは見られないが、このミニFプラスミドに30〜40kb以上の長いDNA断片を挿入すると強く表れることを明らかにした(三木)。

Report

(3 results)
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (78 results)

All Other

All Publications (78 results)

  • [Publications] Adachi N.: "Mutant isolation of mouse DNA topoisomerase lla in yeast." Nucl.Acids.Res.22. 187-188 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kimura K.: "Identification of the nature of modification that causes the shift of DNA topoisomerase IIb to apparent higher molecular weight forms in the M phase." J.Biol.Chem.269. 24523-24526 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ohkata K.: "Cyclin B interaction with microtubule-associated protein 4 (MAP4) targets p34cdc2 kinase to microtubles and is a potential regulator of M-phase microtubule dynamics." J.Cell Biol.(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ohkubo Y.: "SV40 large T antigen reinduces the cell cycle in terminally differentiated myotubes through inducing cdk2,cdc2,and their partner cyclins." Exp.Cell Res.(in press). (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ohsumi K.: "Meiosis-specific cell cycle regulation in maturing Xenopus oocytes." J.Cell Sci.(in press). (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ohmori H.: "The pcsA gene is identical to dinD in Escherichia coli." J.Bacteriol.177. 156-165 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Norris V.: "Cell cycle control:Prokaryotic solutions to eukaryotic problem?" J.theor.Biol.168. 227-230 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Okada Y.: "Cytoplasmic axial filaments in Escherichia coli cells:possible function in the mechanism of chromosome segregation and cell division." J.Bacteriol.176. 917-922 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Asai K.: "CThe identification of Escherichia coli ispB (cel) gene encoding the octaprenyl diphosphate synthase." Biopys.Biochem.Res.Commun.202. 340-345 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Horiuchi T.: "Recombinational rescue of the stalled DNA replication fork;a model based on analysis of an E.coli strain with a chromosmal region difficult to replicate." J.Bacteriol.177. 783-791 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Horiuchi T.: "A new type of E.coli recombinational hotspot which requires for activity both DNA replication termination events and the Chi sequence." Advance in Biophysics. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Horiuchi T.: "The DNA replication fork blocked at the Ter site may be an entrance for the RecBCD enzyme into duplex DNA." J.Bacteriol.176. 4656-4663 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Seki,N.: "Mapping of the human centromere protein B gene (CENPB) to chromosome 20p13 by fluorescence in situ hybridization." Genomics. 24. 187-188 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tatebayashi K.: "Structural analyses of DNA fragments integrated by illegitimate recombination in Schizosaccharomyces pombe." Mol.Gen.Genet.244. 111-119 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 加藤潤一: "大腸菌とλファージの実験法" 細胞工学. 13. 753-756 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hoshino K.: "Inhibition of quinolones against topoisomerase IV from Escherichia coli and comparison with the inhibitory potency against DNA gyrase." Antimicrob.Agents.Chemother.(in press). (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nagata,K.: "Set,aputative oncogene associated with leukemogenesis os required for adenovirus DNA replication." Proc.Natl.Sci.USA. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kikuchi Y.: "A new yeast gene,HTR1,required for high tempereture is needed for recovery from mating pheromone-induced G1 arrest." Hum Mol Genet. 245. 107-116 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamanaka K.: "Multicopy suppressors,mssA and mssB,of an smbA mutation of Escherichia coli." Mol.Gen.Genet.243. 9-16 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamanaka K.: "Characterization of translucent segments observed in an smbA mutant of Escherichia coli." FEMS Microbiol.Lett.166. 61-66 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] YSamanaka K.: "Two mutant alleles of mukB,a gene essential for chromosome partition in Escherichia coli." FEMS Microbiol.Lett.123. 27-32 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamanaka K.: "Cloning,sequencing,and characterization of multicopy suppressors of a mukB mutation in Escherichia coli." Mol.Microbiol.13. 301-312 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ogawa T.: "Cell cycle-dependent transcription from the gid and mioC promoters of Escherichia coli." J.Bacteriol.176. 1609-1615 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ikeno,M.: "Distribution of CENP-B boxes reflected in CREST centromere antigenic sites on long-range α-satellite DNA arrays of human chromosome 21." Hum Mol Genet. 3. 1245-1257 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Miki T.: "Temperature shift-up leads to simultaneous and continuous plamid DNA relaxation and induction of DnaK and GroES proteins in anaerobically growing Escherichia coli cells." FEMS Microbiol.Lett.121. 333-336 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Miki T.: "Identification of DNA topoisomerases involved in immediate and transient DNA relazation induced by heat shock in Escherichia coli." Mol.Gen.Genet.244. 451-455 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Miki T.: "Loss of flageration in dnaA mutants of Escherichia coli." J.Bacteriol.176. 5544-5546 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Miki T.: "Lysozyme requires fluctuation of the active site for the manifestation of activity." Protein Engineering. 7. 743-748 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Miki T.: "Formation of a-helix 88-98 is essential in the establishment of higher-order structure from reduced lysozyme." J.Mol.Biol.235. 1312-1317 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Feng J.: "New killing system controlled by tow genes located immediately upstream of the mukB gene in E.coli." Mol.Gen.Genet.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamanaka K.: "Characterization of translucent segments observed in an smbA mutant of Escherichia coli." FEMS Microbiol.Lett.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamanaka K.: "Multicopy suppressors,mssA and mssB,of an smbA mutation of Escherichia coli." Mol.Gen.Genet.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hiraga S.: "E.coli chromosome partition." Current Opinion in Genetics & Development. 5. 789-801 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ogawa T.: "Cell cycle-dependent transcription from the gid and mioC promoters of Escherichia coli." J.Bacteriol.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Masukata H: "Autonomous replication of human chromosomal DNA fragments in human cells." Cell. 4. 1121-1132 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ohkata K.: "Association of p34^<cdc2>/cycline B complex with microtubules in starfish oocytes." J.Cell Sci.105. 873-881 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kusubata M.: "cdc2 kinase phosphorylation of desmin at three serine/threonine residues in the amino-terminal head domain." Biochem.Biophys.Res.Commun.190. 927-934 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ohsumi K.: "Chromosome condensation in Xenopus mitotic extracts without histone Hl." Science. 262. 2033-2035 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ohta K.: "Microtubule nucleating activity of centrosomes in cell-free extracts from Xenopys eggs:Involvement of phosphorylation and accumulation of pericentriolar material." J.Cell Sci.104. 125-137 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hisanaga S.: "Tau protein kinase II has a similar characteristic to cdc2 kinase for phoshorylating neurofilament proteins." J.Biol.Chem.268. 15056-15060 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kataoka T.: "Fully methylated oriC with negative superhelicity forms an oriC-membran complex before initiation of chromosome replication." Biochem.Biophys.Res.Commun.194. 1420-1426 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tatebayashi K.: "Analysis of integration of DNA fragments by illegitimate recombination in Schizosaccharomyces pombe." Mol.Gen.Genet.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 加藤潤一: "大腸菌のDNAトポイソメラーゼIV." 細胞工学. 1. 19-26 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Masuda Y.: "Isolation of temperature-sensitive aminoacyl-tRNA synthetase mutants from an Escherichia coli strain harboring the pemK plasmid." Mol.Gen.Genet.238. 169-176 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tsychimoto S.: "The stable maintenance system pem of plasmid R 100:Degradation of PemI protein may allow PemK protein to inhibit cell growth." J.Bacteriol.174. 4205-4211 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Masuda Y.: "chpA and chpB,Escherichia coli chromosomal homologues of the pem locus responsible for stable maintenance of plasmid R 100." J.Bacteriol.175. 6850-6856 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Higashitani A.: "Osmoregulation of the fatty acid receptor gene fadL in Escherichia coli." Mol.Gen.Genet.240. 339-347 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tomura A.: "Requirement of phosphatydilglycerol for flagellation of Escherichia coli." FEBS Letters. 329. 287-290 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ueda T.: "Stabilization of lysozyme by the introduction of Gly-Pro sequence." Protein Engineering. 6. 183-187 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ogata Y.: "A role of heat shock proteins for homologous recombination in Escherichia coli." Biochem.Biophys.Res.Comm.197. 34-39 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 真木智子: "DNAジャイレースの活性を調節する蛋白質" 細胞工学. 12. 10-18 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Herman C.: "Cell growth and lambda phage development controlled by the same essential Escherichia coli gene,ftsH/hflB." Proc.Natl.Acad.Sci.USA. 90. 10861-10865 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tomoyasu T.: "Topology and subcellular localization of FtsH protein in Escherichia coli." J.Bacteriol.175. 1352-1357 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nishitani H.: "Specific chromosomal sites enhancing homologous recombination in Escherichia coli mutants defective in RNaseH." Mol.Gen.Genet.240. 307-314 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Akiyama,Y.: "Involvement of FtsH in protein assembly into and through the membrane.1.Mutation that reduce retention efficiency of a cytoplasmic reporter." J.Biol.Chem.in press. (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tomoyasu,T.: "The Escherichia coli FtsH protein is a prokaryotic member of a protein family of putative ATPases involved in membrane functions,cell cycle control and gene expression." J.Bacteriol.175. 1344-1351 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Masumoto H.: "Properties of CENP-B and its target sequence in satellite DNA,In B.K.Vig(ed),Chromosome Segregation and Aneuploidy." Springer-Verlag,Berlin, 12 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kishimoto T.: "Control of M-phage by MPF/cyclin-dependent cdc2 kinase.In H.Mohri,M.Takahashi and M.Inagaki(ed),Biology of the Germ Line-Animal and Man." Japan SAcientific Societies Press,Tokyo, 16 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nishimura,A.,Akiyama,K.,Kohara,Y.and Horiuchi,K.: "Correlation of a subset of the pLC-plasmids to the physical map of Escherichia coliK-12" Microbiol.Rev.56. 137-151 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yamanaka,K.,Ogura,T.,Niki,H.and Hiraga,S.: "Identification and characterization of the smbA gene,a suppressor of the mukB mull mutant in Escherichia coli." J.Bacteriol.174. 7517-7525 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yoda,K.,Kitagawa,K.,Masumoto,H.,Muro,Y.and Okazaki,T.: "A human centromeric protein,CENP-B,has a new DNA binding motif containing four potential a-helices at the N-terminus which is separable from dimerizing activity." J.Cell.Biol.119. 1413-1427 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Watanabe,E.,Wachi,M.,Yamasaki,M.and Nagai,K.: "ATPase activity of SopA,a protein essential for active partitioning of F plasmid." Mol.Gen.Genet.234. 346-352 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tsuchimoto,S.,Nishimura,Y.and Ohtsubo,E.: "The stable maintenance system pem of plasmid R100:Degradation of Peml protien may allow PemK protien to inhidit cell growth." J.Bacteriol.174. 4205-4211 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Begg,K.J.,Tomoyasu,T.,Donachie,W.D.,Khattar,M.,Niki,H.,Yamanaka,K.,Hiraga,S.and Ogura,T.: "Escherichia coli mutant Y16 is a double mutant carrying thrmosensitive ftsH and ftsl mutations." J.Bacteriol.174. 2416-2417 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kambe-Honjoh,H.,Tamura,G.and Nagai,K.: "Association of oriC region of Escherichia coli chromosome with outer membrane:effects of culture condition." Biochem.Biophys.Res.Commun.(1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Fujii-Nakata,T.,Ishimi,Y.,Okuda,A.and Kikuchi,A.: "Functional analysis of nucleosome assembly protien,NAP-1:Negatively charged C-terminal region is not necessary for the intrinsic assembly activity." J.Biol.Chem.267. 20980-20986 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Niki,H.,Imamura,R.,Kitaok,M.,Yamanaka,K.,Ogura,T.and Hiraga,S.: "E.coli MukB protein involved in chromosome partition forms a homodimer with a rod-and-hinge structure naving DNA binding and ATP/GTP binding activities." EMBO J.11. 5101-5109 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Muro,Y.,Masumto,H.,Yoda,K.,Mozaki,N.,Ohashi,M.and Okazaki,T.: "Centromere protein B assembles human centromeric a-satellite DNA at the 17-bp sequence,CENP-B box." J.Cell Biol.116. 585-596 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Imamura,R.,Niki,H.,Kitaoka,M.,Yamanaka,K.,Ogura,T.and Hiraga,S.: "Caracterization of high molecular weights of complexes and polymers of cytoplasmic proeins in Escherichia coli." Res.Microbiol.143. 743-753 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kaidow,A.,Kataoka,T.,Wachi,M.,Takada,A.,Yamasaki,M.and Nagai,K.: "The 55-kilodalton protein in an oriC complex fraction is glycogen synthese." J.Bacteriol.174. 5454-5456 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Okada,Y.,Wachi,M.,Ymasaki,M.,and Nagai,K.: "Change of the quantity of penicillin-binding proteins and other cytoplasmic and membrane proteins by mutations of the cell shape-determination genes mreB,BreC and mreD of Escherichia coli." J.Appl.Microbiol.38. 157-163 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 和知 正明,永井 和夫: "大腸菌セプタム形成に関与する蛋白質。" Radioisotopes. 41. (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Takahashi,K.,Murakami,S.,Chikashidga,Y.,funabiki,H.,Niwa,O.,and Yanagida,M.: "A low copy number centrial sequence with strict symmetry and unusual chromatin structure in fission fission yeast centromere." Mol.Biol.Cell.

    • Related Report
      1992 Annual Research Report
  • [Publications] Tomoyasu,T.,T.Yuki,S.Morimura,H.Mori,K.Yamanaka,H.Niki,S.Hiraga,and T.Ogura.: "The Escherichia coli FtsH protein is a prokaryotic member of a protein family of putative ATPases involved in membrane functions,cell cycle control and gene expression." J.Bacteriol.175. 1344-1351 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tomoyasu,T.,K.Yamanaka,K.Murata,T.Suzaki,P.Bouloc,A.Kato,H.Niki,S.Hiraga,and T.Ogura.: "Topology and subcellular localization of FtsH protein in Escherichia coli." J.Bacteriol.175. 1352-1357 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hiraga,S.: "Chromosome and plasmid partition in Escherichia coli.“Annual Review of Biochemistry"" C.C.Richardson,J.N.Abelson,A.Meiste椅 and C.T.Walsh,eds.,Annual Reviews Inc.Palo Alto,California., 14 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kikuchi,A.,Miyaike,M.: "Expression of human type I and II topoisomerases and their derivatives in Eschenchia coli.in “Molecular Biology of DNA topoisomerase and its Application to Chemotherapy."" Andoh,T.,Ikeda,H.,and Ogura,M.,eds.,CRC Press Inc.Florida,USA, 10 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kato,J.,and Ikeda,H.: "Purification and characterization of DNA topoismerase IV in Escherichia coli.in Escherichia coli.in “Molecular Biology of DNA topoisomerase and its Application to Chemotheraoy."" Andoh,T.,Ikeda,H.,and Ogura,M.,eds.,CRC Prees Inc.Florida,USA, (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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