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Molecular mechanism of the elasticity of plant gene expression and signal transduction chains

Research Project

Project/Area Number 04257101
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionGraduate School of Science, University of Tokyo

Principal Investigator

NAGATA Toshiyuki  Univ.of Tokyo, Prof., 大学院・理学系研究科, 教授 (10012519)

Co-Investigator(Kenkyū-buntansha) WATANABE Akira  Univ.of Tokyo, Prof., 大学院・理学系研究科, 教授 (70023471)
OKADA Yoshimi  Teikyo Univ.Prof., 理工学部, 教授 (30011703)
NAKAMURA Kenzo  Nagoya Univ.Prof., 農学部, 教授 (80164292)
MIKAMI Tetsuo  Hokkaido Univ.Prof., 農学部, 教授 (50133715)
UCHIMIYA Hirofumi  Univ.of Tokyo, Prof., 分子細胞生物学研究所, 教授 (50142229)
岩淵 雅樹  京都大学, 理学部, 教授 (30000839)
Project Period (FY) 1991 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥36,500,000 (Direct Cost: ¥36,500,000)
Fiscal Year 1994: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1993: ¥13,200,000 (Direct Cost: ¥13,200,000)
Fiscal Year 1992: ¥20,300,000 (Direct Cost: ¥20,300,000)
KeywordsPlant hormones / Auxin / Signal transduction / Transcriptional regulation / G proteins / Protein kinases / Stress response / Tobacco BY-2 cell line / 形質転換植物 / タバコ培養細胞株BY-2 / 転写制御因子 / 細胞分化 / エリシター / トランスジェニック植物 / シロイヌナズナ / プロテインキナーゼ / タバコ
Research Abstract

Plants have unique characteristics in various aspects in compared to animals, as they are composed of cells covered by thick cell walls. Plants cannot move in response to environmental changes, however, plants have developed characteristic strategy against environmental changes. This project was proposed to elucidate the molecular mechanisms underlying these unique characteristics. When the term of this project finished, we have had some confident results in some aspects as follows. First, we identified parB as one of auxin-regulated genes, which was found to encode glutathione S-transferase. This is the first case, in which any one of auxinregulated genes has been ascribed to apecific functions. We have also isolated acrA as another auxin-regulated gene, which was found to encode a beta-subunit-like protein of G proteins. This discovery opened new area, in which signal transduction chains mediated by protein kinase C are also involved in plant hormone-regulated phenomena, as the gene product of arcA is likely to be a receptor for activated C kinases. On the other hand, as for the signal transduction chains regarding phytopathogenic processes, a receptor for an elicitor was identified, while some aspects of phosphoinositide metabolisms was also elucidated. In this regard, regeneration of resistant plants against pathogenic fungi was also tried. It was also demonstrated that some specific gene product of virus genomes was involved in the acquisition of resistance of plants against virus infection. Thus, overall, this project attained some positive results in the field of plant molecular biology. As a kind of byproducts, tobacco BY-2 cells have been internationally recognized as model experimental materials in plants through the activity of researchers of this project, as it was established that the cell line grows very fast and can be synchronized in a strict sense and found to be useful for the analysis of basic aspects of plant cells.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (35 results)

All Other

All Publications (35 results)

  • [Publications] Takahashi,Y.,Hasezawa,S.,Kusaba.M.& Nagata,T.: "Expression of the auxin-regulated parA gene in transgenic tobacco and nuclear localization of its gene products." Planta. 196. 111-117 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takahashi,Y.,Sakai,T.,Ishida,S.& Nagata,T.: "Identification of auxin-responsive elements of parB and their expression in apices of shoot and root." Proc.Natl.Acad.Sci.USA. 92. in press (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Demura,T.& Fukuda,H.: "Novel vascular cell-specific genes whose expression is regulated temporally and spatially during vascular system development." Plant Cell. 6. 967-981 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nagata,T.,Ishida,S.,Hasezawa,S.& Takahashi,Y.: "Genes involved in the dedifferentiation of plant cells." Int.J.Dev.Biol.38. 321-327 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ishiguro,S.& Nakamura,K.: "Characterization of a cDNA encoding a novel DNA-binding protein,SPF1,that recognizes SP8 sequences in the 5'upstream regione of genes coding for sporamin and β-amylase from sweet potato." Mol.Gen.Genet.244. 563-571 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Fujii,N.,Yokoyama,R.& Uchimiya,H.: "Analysis of the rolC promoter region involved in somatic embryogenesis-related activation in carrot cell culture." Plant Physiol.104. 1151-1157 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 長田敏行: "植物プロトプラストの細胞工学" 講談社, 132 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 長田敏行・内宮博文(編): "植物の遺伝子発現" 講談社, 238 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takahashi, Y., Hasezawa, S., Kusaba, M.and Nagata, T.: "Expression of the auxin-regulated ParA gene in transgenic tobacco and nuclear localization of its gene products." Planta. 196. 111-117 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takahashi, Y., Sakai, T., Ishida, S.and Nagata, T.: "Idenfification of auxin-responsive elements of parB and their expression in apices of shoot and root." Proc.Natl.Acad.Sci.USA. 92 (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Demura, T.and Fukuda, H.: "Novel Vascular cell-specific genes whose expression is regulated temporally and spatially during vascular system developments." Plant cell. 6. 967-981 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nagata, T., Ishida, S., Hasezawa, S.and Takahashi, Y.: "Genes involved in the dedifferentiation of plant cells" Int.J.Dev.Biol.38. 321-327 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ishiguro, S.and Nakamura, K.: "Characterization of a cDNA encoding a novel DNA-binding protein, SPF1, that recognizes SP8 sequences in the 5' upstream regions of genes coding for sporamin and beta-amylase from sweet potato." Mol.Gen.Genet.244. 563-571 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Fujii, N., Yokoyama, R.and Uchimiya, H.: "Analysis of the rolC promoter region involved in somatic embryogenesis-related activation in carrot cell culture." Plant Physiology. 104. 1151-1157 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ishida, S., Takahashi, Y.and Nagata, T.: "Isolation of cDNA of an auxin-regulated gene encoding a G protein beta-subunit-like protein from tobacco BY-2 cells." Proc.Natl.Acad.Sci.USA. 90. 11152-11156 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takahashi, Y.and Nagata, T.: "parB : An auxin-regualted gene encoding glutathione S-transferase." Proc.Natl.Acad.Sci.USA. 89. 56-59 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takahashi,Y. et al.: "Expression of the auxin-regulated parA gene in transgenic tobacco and nuclear lecalization of its gene products." Planta. (印刷中).

    • Related Report
      1994 Annual Research Report
  • [Publications] Nagata,T. et al.: "The origin and organization of cortical microtubules during the transition from M and G_1 phases of the cell cycle as observed in highly synchronized cells of tobacco BY-2." Planta. 193. 567-572 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takahashi,Y. et al.: "Function and modulation of expression of auxin-regulated genes." Internatl.Rev.Cytol.152. 109-138 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nagata,T. et al.: "Genes involved in the dedifferentiation of plant cells." Internatl.J.Develop.Biol.38. 321-327 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kidou,S. et al.: "Isolation and characterization of a rice cDNA similar to the S-phase-specific cyc07 gene." Plant Mol.Biol.24. 545-547 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ishiguro,S.& Nakamura,K.: "Characterization of a cDNA encoding a novel DNA-binding protein, SPFl,that recognizes SP8 sequences in the 5-upstream regions of genes encoding for sporamin and β-amylase from sweet potato." Mol.Gen.Genet.244. 563-571 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 長田敏行、内宮博文(編): "植物の遺伝子発現" 長田敏行、内宮博文(編), 238 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ishida,S.,Takahashi,Y.& Nagata,T.: "Isolation of cDNA of an auxin-regulated gene encoding a G protein β subunit-like protein from tobacco BY-2 cells." Proc.Natl.Acad.Sci.USA. 90. 11152-11156 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nagata,T.,Ishida,S.,Hasezawa,S.& Takahashi,Y.: "Genes involved in the dedifferentiation of plant cells." Internatl.J.Develop.Biol.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Uchimiya,H.et al.: "Bialaphos treatment of transgenic rice plants expressing a bar gene prevents infection by the sheath blight pathogen." Bio/Technology. 34. 835-836 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ishiguro,S.& Nakamura,K.: "Characterization of a cDNA encoding a novel DNA-binding protein that recognizes SP8 sequences in the 5'-" Mol.Gen.Genet.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Fukuda,H.: "Tracheary element formation as a model system of cell differentiation." Intnatl.Rev.Cytol.136. 289-332 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamaguchi-Shinozaki,K.: "A novel cis acting element in an Arabidopsis genes is involved in responsiveness to drought,low temperature or high salt stress." Plant Cell. (in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yohsuke Takahashi: "parB:An auxin-regulated gene encoding glutathione S-transferase." Proc.Natl.Acad.Sci.USA. 89. 56-59 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kazuo Shinozaki: "Molecular cloning and nucleotide sequence of a gene encoding a myb homologue in Arabidopsis thaliana." Plant Mol.Biol.19. 493-499 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kiyotaka Okada: "Aspects of recent developments in mutational studies of plant signaling pathways." Cell. 70. 369-392 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Maki Kawai: "Molecular characterization of cDNA encoding for adenylate kinase of rice(Oryza sative L.)." Plant Journal. 2. 1005-1009 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Masaaki Yoshikawa: "A specific binding site on soybean membranes for a phytoalexin elicitor released from fungal cell walls by β-1,3-endoglucanaso." Plant Cell.

    • Related Report
      1992 Annual Research Report
  • [Publications] Hisayuki Mutsui: "A gene family,kat,encoding kinesin-like proteins in Arabidopsis thaliana and the secondary structure of KatA protein." Mol.Gen.Genet.

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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