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血管系の機能および病変の基盤としての内皮平滑筋連関

Research Project

Project/Area Number 04263105
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionKyoto University

Principal Investigator

眞崎 知生  京都大学, 医学部, 教授 (60009991)

Co-Investigator(Kenkyū-buntansha) 後藤 勝年  筑波大学, 基礎医学系, 教授 (30012660)
北 徹  京都大学, 医学部, 教授 (60161460)
室田 誠逸  東京医科歯科大学, 歯学部, 教授 (50072989)
野々村 禎昭  東京大学, 医学部, 教授 (80009993)
江橋 節郎  岡崎国立共同研究機構, 名誉教授 (10009863)
多田 道彦  大阪大学, 医学部, 教授 (90093434)
平 則夫  東北大学, 医学部, 教授 (60004553)
井村 裕夫  京都大学, 総長 (10025570)
山野 俊雄  大阪大学, 医学部, 名誉教授 (40028270)
Project Period (FY) 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥64,000,000 (Direct Cost: ¥64,000,000)
Fiscal Year 1994: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1993: ¥32,000,000 (Direct Cost: ¥32,000,000)
Fiscal Year 1992: ¥29,000,000 (Direct Cost: ¥29,000,000)
Keywords血管内皮 / 平滑筋 / 内皮由来生理活性物質 / 血管病変 / 血管収縮弛緩 / 増殖因子 / 収縮弛緩 / 血管生物学 / 血管攣縮 / 動脈変化 / 血圧 / 内皮細胞 / 平滑筋細胞 / 動脈硬化 / エンドセリン / EDRF / ANP
Research Abstract

重点領域研究「血管内皮平滑筋」の3年間の研究成果を本研究計画の中で総括した。3年間の研究機関にいくつかの重要な研究成果があがったことが確認された。内皮由来の血管収縮弛緩因子の研究は世界的にも一酸化窒素とエンドセリンが中心となっている。本研究計画の中ではエンドセリンに関する研究が著しく進展した。またエンドセリン変換酵素のクローニングも行われた。また特にエンドセリンノックアウトマウス作成の報告はエンドセリンの生理的意義を考える上でひとつの方向を与える者である。平滑筋研究に関しては収縮弛緩機構に関する基礎的研究の進展がみられた上に、平滑筋細胞の形質変換に関して分子レベルからの研究が一段と進歩した。また全身的調節機構の観点からはナトリウム利尿ペプチドファミリー、エンドセリンファミリー、一酸化窒素の相互作用、あるいは基礎的研究を中心に進められ、血管攣縮、血圧調節動脈硬化、循環調節などの臨床的観点から、これらの因子をどう位置づけるかという点できわめて貴重な研究成果が得られた。本研究計画の中では内皮細胞の機能に関する知識の蓄積が行われたことが重要である。また本研究課題では多くの公募研究課題の応募があり、計画研究とあわせて、わが国の血管研究の進展にきわめて重要な学問分野の形成、また研究活動の集約に役立ったことをあげなければいけない。本研究課題の終了にあたって、この学問領域のさらなる発展のために、これまでの研究成果の中から重要な問題を選んでこれを展開させることがきわめて重要であると結論された。

Report

(3 results)
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] I.Saito: "Reduction of nitric oxide producing activity associated with in vitro aging in cultured human umbilical vein endothelial cell." Biochem.Biophys.Res.Commun.195. 1070-1076 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Oikawa: "Radiciol,a microbial cell differentiation modulator,inhibits in vivo angiogenesis." Eur.J.Pharmacol.241. 221-227 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Fujita: "A possible mechanism for vascular endothelial cell injury elicited byactivated leukocytes:A significant involvement of adhesion molecules,CD11/CD18 and ICAM-" Arch.Biochem.Biophys.309. 62-69 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Takigawa: "Molecular identification of G-proteins which couple to endothelin receptors." Eur.J.Biochem.(in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Enoki: "Long-lasting activation of cation current by endothelin-1 in smooth muscle cells of rabbit aorta and mouse fibroblasts." Brit.J.Pharmacol.(in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Takagi: "Structural basis of G Protein specificity of human endothelin receptors:a study with endothelin A/B chimeras." J.Biol.Chem.(in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Masaki: "Endothelium-Derived Factors and Vascular Functions" Excerpta Medica, (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Masaki: "Annual Review of Pharmacology and Toxicology" Annual Reviews Inc (in press), 6

    • Related Report
      1994 Annual Research Report
  • [Publications] Sakamoto,T.: "Distinct subdomains of human endothelin receptors determine their selectivity to endothelin _A-selective antagonist and endothelin _B-selective agonist" J.Biol.Chem.268. 8547-8553 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kusunoki,S.: "Generation of a monoclonal antibody specific for a new class of minor ganglioside antigens,GQ1ba and GT1aα:its binding to dorsal and lateral horn of human thoracic" Brain Research. 623. 83-88 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sato,I.: "Reduction of nitric oxide producing activity associated with in vitro aging in cultured human umbilical vein endothelial cell" Biochem.Biophys.Res.Commun.155. 1070-1076 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tanaka,M.: "Regulation of apolipoprotein B production and secretion in response to the change of intracellular cholesteryl ester contents in rabbit hepatocytes" J.Biol.Chem.268. 12713-12718 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tanokura,M.: "Complexes of myosin subfragment 1 with pyrophosphate and with adenosine diphosphate as studied by phosphorus-31 nuclear magnetic resonance" J.Biochem.113. 19-21 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tsunoda,H.: "Mechanism of rat uterine smooth muscle contraction induced by endothelin-1" Br.J.Pharmacol.110. 1437-1440 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Masaki,T.: "Endothelin as a vascular hrmone in Progress in Endocrinology" Parthenon Publishing, 3 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hosoda,K.: "Organization structure,chromosomal,assignment and expression of the gene encoding the human endothelin-A receptor" J.Biol.Chem.267. 18797-18804 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sakurai,T.: "Level of ETB receptor mRNA is down-regulated by endothelins through decreasing the intracellular stability of mRNA molecules" Biochem.Biophys.Res.Commun.186. 342-347 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Satoh,K.: "The group at C2 of N-thylnicotinamide determines the vasodilator potencies and mechanisms of action of nicorandil and its congeners in canine coronary artery" Nannyn-Schnriedeberg's arch Pharmacol.344. 589-595 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Xiong,Z.: "Evidence for contribution of Ca^<2+> storage sites on urinaty K channel currents in inside-out membrane of rabbit portal vein" Pflung.Arch.420. 112-114 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kobayashi,H.: "Isolation of cDNA for bovine stomach 155KDa protein exhibitting myosin light chain kinase activity" J.Biochem.112. 786-791 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kasuya,Y.: "A pertussis toxin-sensitive mechanism of endothelin action in porcine coronary artery smooth muscle" Br.J.Pharmacol.107. 456-462 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] G.Rubanyi: "Endothelin" Oxford Univ.Press, 277 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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