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イオン・溶質輸送と共役ネットワーク

Research Project

Project/Area Number 04266101
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionTokyo Metropolitan Organization for Medical Research

Principal Investigator

川喜田 正夫  財団法人東京都臨床医学総合研究所, 医化学研究部門, 研究員 (00012740)

Co-Investigator(Kenkyū-buntansha) 原 諭吉  東京医科歯科大学, 医学部, 助教授 (00092429)
山口 明人  千葉大学, 薬学部, 助教授 (60114336)
重川 宗一  国立循環器センター研究所, 循環分子生理部, 研究員 (00113738)
青島 均  山口大学, 教養部, 教授 (20089907)
乾 賢一  京都大学, 医学部, 教授 (70034030)
水上 茂樹  九州大学, 医学部, 教授 (90037325)
星 猛  静岡県立大学, 食品栄養科学部, 教授 (60004537)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥149,000,000 (Direct Cost: ¥149,000,000)
Fiscal Year 1994: ¥54,000,000 (Direct Cost: ¥54,000,000)
Fiscal Year 1993: ¥42,000,000 (Direct Cost: ¥42,000,000)
Fiscal Year 1992: ¥53,000,000 (Direct Cost: ¥53,000,000)
Keywords共役物質輸送 / Na^+ / H^+アンチポーター / H^+ / ジペプチドシンポーター / Band 3 / テトラサイクリン / アラニンキャリアー / バクテリオロドプシン / K^+-ATPase / イオン輸送 / H^+-アンチポーター / ジペプチド輸送系 / H^+ヘキソース共輸送 / テトラサイクリン排出タンパク質 / H^+ーアンチポーター / 酸性アミノ酸輸送系 / K^+ーATPase
Research Abstract

細胞の膜系における各種のイオンや溶質の共役輸送系の実体とそのネットワークに関して、以下の結果を得た。
1.Na^+/H^+-アンチポーター(NHE)のisoformに対する特異的ペプチド抗体を作成し、各isoformの組織における局在性を明らかにした(川喜田)。NHE1 isoformの活性の細胞内pHによる調節の情報がカルモジュリンによって伝達されることを示し、調節に関与する領域を同定した(重川)。腸炎ビブリオNHEのアミロライド結合部位の変異体では、NHEのイオン特異性が同時に変化することを明らかにした(土屋)。
2.H^+/ジペプチドシンポーターのcDNAクローニングに成功し、塩基配列を決定した(乾)。腎上皮細胞による有機アニオンの経細胞輸送のin vitro実験系を確立し、プロテインキナーゼCによる活性調節を明らかにした(高野)。
3.赤血球膜のアニオン輸送体Band 3の構造をタンパク質化学的に解析し、新しい構造モデルを提案した。(濱崎)。
4.テトラサイクリン/H^+アンチポーターのトポロジーおよび反応機構を、復帰変異解析、Cys走査変異体の解析によって詳しく研究し、精細な構造モデルを発表した(山口)。
5.好熱菌のアラニンキャリアーの大量発現に成功した。さらに結晶化による詳細な構造解析の方向を指向している(平田)。
6.Na^+/K^+-ATPaseのαβ分子集合体形成機構について、H^+/K^+-ATPaseとのキメラを用いて解析し、N末50残基については互換性があることを明らかにした(川村)。また、α1およびα3 isoformの間の特性のちがいがNa^+遊離サイトの基質親和性のちがいに基づくものであることを明らかにした(原)。
7.バクテリオロドプシンのH^+移動反応に関与するアミノ酸残基として、Asp85,Asp212,Asp96/Thr46,Trp182を同定することに成功した(前田)。

Report

(3 results)
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (107 results)

All Other

All Publications (107 results)

  • [Publications] Ono,A: "Transport of envelope proteins of Sendai virus HN and FO,is blocked at different steps by thapdigargin and other perturbants to intracellular Ca2+" J.Biochem.116. 649-656 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Aoki,K: "Isolation and characterization of a novel mutant mouse cell line resistant of Newcastle disease virus:constitutive interferon production and enhanced interferon sensitivity" Arch.Virol.139. 337-350 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Suzuki,T: "Sites of labeling with N-(3-pyrene)maleimide on Ca^<2+>-transporting ATPase of the Saroplasmic reticulum" J.Biochem.117. 881-887 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Wada,M.: "Molecular cloning of P-type ATPases on intracellular membranes of the marine alga Heterosigma akashiwo" Plant Mol.Biol. 26. 699-708 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sumimoto,H.: "Role of Src homology domains in assembly and activation of the phagocyte NADPH oxidase" Proc.Natl.Acad.Sci.,U.S.A.91. 5345-5349 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hashimoto,Y.: "Functional evidence for an apical ANP receptor in LLC-PK_1 kidney epithelial cells" Eur.J.Pharmacol. 268. 443-445 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Matsumoto,S.: "Transcellual transport of oral cephalosporins in human intestinal epithelial cells,Caco-2:interaction with dipeptide transport systems in apical and basolateral membranes" J.Pharmacol.Exp.Ther.270. 498-504 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sasaki,S.: "Cloning,characterization,and chromosomal mapping of human aquaporin of collecting duct" J.Clin.Invest.93. 1250-1256 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Aoshima,H.: "Effects of aliphatic alcohols and food additives on nicotinic acetylcholine receptors in Zenopus oocytes" Biosci.Biothech.biochem.58. 1776-1779 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Okubo,K.: "Red blood cell Band 3" J.Biol.Chem.269. 1918-1926 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kato,S.: "Analysis of mdr-1 gene expression in human leukemic cells by quantitative PCR" Leukemia and Lymphoma. 14. 129-135 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ideguchi,H.: "A comprehensive scheme for the systematic investigation of hemolytic anemia" Ann.Clin.Lab.Sci.24. 412-421 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Takano: "Transport of gentamicin and fluid-phase endocytosis markers in the LLC-PK_1 kidney epithelial cell line" J.Pharmacol.Exp.Ther.268. 669-674 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Takano: "Bestatin transport in rabbit intestinal brush-border membrane vesicles" Biochem.Pharmacol.47. 1089-1090 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Takano: "Cellular toxicity of aminoglycoside antibiotics in G418-sensitive and -resistant LLC-PK_1 cells" Pharm.Res.11. 609-615 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Takano: "p-Aminohippurate transport in apical and basolateral membranes of the OK kidney epithelial cells" J.Pharmacol.Exp.Ther.269. 970-975 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Takano: "Differential sensitivity of organic anion transporters in rat renal brush-border membrane to diethyl pyrocarbonate" Biochim.Biophys.Acta. 1192. 190-196 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Tomita: "Transport of oral cephalosporins by the H^+/dipeptide cotransporter and distribution of the transport activity in isolated rabbit intestinal epithelial cells." J.Pharmacol.Exp.Ther.272. 63-69 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Wakabayashi,S.: "Growth factor activation and "H^+-sensing" of the Na^+/H^+ exchange isoform 1(NHE1).Evidence for an additional mechanism not requiring direct phosphorylation." J.Biol.Chem.269. 5583-5588 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Bertrand,B.: "The Na^+/H^+ exchanger isoform 1(NHE1)is a novel member of calmodulin binding proteins." J.Biol.Chem.269. 13703-13709 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Wakabayashi,S.: "Mutation of calmodulin-binding site renders the Na^+/H^+ exchanger(NHE1)highly H^+-sensitive and Ca^<2+> regulation-defective." J.Biol.Chem.269. 13710-13715 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ohsima,N.: "Regulation of Ca^<2+> entry in rat aortic smooth muscle cells in primary culture." J.Biochem.116. 274-281 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Iwata,Y.: "Ca^<2+>-ATPase distributes differently in cardiac sarcolemma than dihydropyridine receptor α 1 subunit and Na^+/Ca^<2+> exchanger." FEBS Lett.355. 65-68 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 岩本隆宏: "培養血管平滑筋細胞L型電位依存性Ca^<2+>チャンネルのリン酸化による制御" 血管. 17. 93-101 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] I.Yamato: "Site-specific alteration of arginine-376,the unique positively charged amino acid residue in the mid-membrane spanning regions of the proline carrier of E.coli." J.Biol.Chem.269. 5720-5724 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Takase: "Sequencing and characterization of the ntp gene cluster for vacuolar type Na^+-translocating ATPase of Enterococcus hirae." J.Biol.Chem.269. 11037-11044 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 山登一郎: "タンパク質をデザインする一生命科学における第2の暗号解読問題" Sut Bulletin. 8. 36-41 (1994)

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      1994 Annual Research Report
  • [Publications] Y.Komeiji: "Clycine-85 of the trp-repressor of E.coli is important in forming the hydrophobic tryptophan binding pocket:experimental and computational approaches." Prot.Engng.7. 1239-1247 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Komeiji: "Molecular dynamics simulations of trp apo-and holorepressors:domain structure and ligand-protein interaction." Proteins:Structure,Function,and Genetics. 20. 248-258 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 丸山明子: "酸化LDLのスーパーオキサイド産生能及び好中球からのスーパーオキサイド産生誘導能" 帝京医学雑誌. 17. 511-521 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Noriko Okazaki: "Characterizaion of the lactose transport system in Citrobacter freundii." Biol.Pharm.Bull.17(6). 794-797 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Teruo Kuroda: "Properties of the Na^+/H^+ antiporter in Vibrio parahaemolytics." J.Biochem.115. 1162-1165 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Toshi Shimamoto: "The NhaB Na^+/H^+ antiporter is essential for intracellular pH regulation under alkaline condition in Escherichia coil." J.Biochem.116. 285-290 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Teruo Kuroda: "Properties and sequence of the NhaA Na^+/H^+ antiporter of Vibrio parahaemolyticus." J.Biochem.116. 1030-1038 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Jong-ln Lee: "C loning and sequence of the gene for the lactose carrier of Citrobacter freundii." Biochem.Biophys.Res.Commun.203(3). 1882-1888 (1994)

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      1994 Annual Research Report
  • [Publications] Rafiquel l.Sarker: "Characterization of a glucose transport system in Vibrio parahaemolyticus." J.Bacteriol.176. 7378-7382 (1994)

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      1994 Annual Research Report
  • [Publications] Kamata,H.: "Amphiphilic Peptides Enhance thd Efficiency of Liposome-mediated DNA Transfection." Nucleic Acids Research. 22. 536-537 (1994)

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      1994 Annual Research Report
  • [Publications] Nakanishi,M.: "Purification and Reconstitution of an Intestinal Na^+-dependent Neutral Amino Acid L-α-Amino Acid Transporter." J.Biol.Chem.269. 9325-9329 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yoshida,N.: "Effect of Calcium on the Mobility of γ-Chain from Fibrinogen Osaka V on Sodium Dodecyl Sulfate-Polyacrylamide Gel.Electrophoresis." Throbosis Res.73. 79-82 (1994)

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      1994 Annual Research Report
  • [Publications] Yagisawa.H.: "Expression and Characterization of an Inositol 1,4,5-Trisphosphate Binding Domain of Phosphatidylinositol-specific Phospholipase C-δ 1" J.Biol.Chem.269. 20179-20188 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shinomiya,H.: "Identification of the 65-kDa Phosphoprotein in Murine Macrophages As a Novel Protein:Homology with Human L-Plastin." Biochem.Biophys.Res.Commun.202. 1631-1638 (1994)

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      1994 Annual Research Report
  • [Publications] Ohta,T.: "Molecular Cloning of Two New Heat Shock Genes Related to the hsp70 Genes in Staphylococcus aureus." J.Bacteriol.176. 4779-4883 (1994)

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      1994 Annual Research Report
  • [Publications] Maeda,A.: "Interaction of aspartate-85 with a water molecule and the protonated Schiff base in the L intermediate of bacteriorhodopsin:A Fourier transform infrared spectroscopic study." Biochemistry. 33. 1713-1717 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sasaki,J.: "Complete identification of C=O stretching vibrational bands of protonated aspartic acid residues in the difference infrared spectra of M and N intermediates versus bacteriorhodpsin." Biochemistry. 33. 3178-3184 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Brown,L.S.: "The proton transfers in the cytoplasmic domain of bacteriorhodopsin are facilitated by a cluster of interacting residues." J.Mol.Biol.239. 401-414 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Brown,L.S.: "The retinal Schiff base-counterion complex of bacteriorhodopsin:Changed geometry during the photocycle is a cause of proton transfer to aspartate 85." Biochemistry. 33. 12001-12011 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kataoka,M.: "Energy coupling in an ion pump:the reprotonation switch of bacteriorhodopsin." J.Mol Biol.243. 621-638 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamazaki,Y.: "Interaction of tryptophan-182 with the retinal 9-methyl group in the L intermediate of bacteriorhodopsin." Biochemistry. 34. 577-582 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamaguchi,A.: "Hot spots for sulfhydryl inactivation of Cys mutants in the widely conserved sequence motifs of the meta-tetracycline/H^+....." J.Biochem.115. 958-964 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sato,K.: "Tetracycline/H^+ antiporter was degraded rapidly in Escherichia coli cells when truncated at last transmembrane helix ....." Biochem.Biophys.Res.Commun.202. 258-264 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Someya,Y.: "Site-specificity of the second-site suppressor mutation of the Asp285- Asn mutant of metal-tetracycline/H^+ antiporter of ....." Biochemistry. 34. 7-12 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Someya,Y.: "A novel glycylcycline,9-(N,N-dimethylglycylamido)-6-demethyl-6-deoxytetracycline,is neither transported nor recognized by.." Antimicrob.Agents Chemother.39. 247-249 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kawano,H.: "Phenytoin,antiepileptic drug,competitively blocked non-NMDA receptors produced by Xenopus oocytes." Neuroscience Lett.166. 183-186 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Noguchi,S.: "The functional roles of disulfide bonds in the β-subunit of (Na,K)ATPase as studied by site-directed mutagenesis" FEBS Lett.341. 233-238 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Morohashi,M.: "Expression of (Na,K)ATPase/Ca-ATPase chimeric proteins in Xenopus oocytes revealed that the COOH-terminal one-third of the (Na,K)ATPase α-subunit is involved in assembly with the β-subunit" J.UOEH. 16. 277-286 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ueno,S.: "Functional consequences of substitution of the disulfide-bonded segment,cys127cys150,located in the extracellular domain of the Na,K-ATPase β subunit:arg148 is essential for the functional expression of Na,K-ATPase" J.Biochem. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 濱崎直孝: "赤血球膜バンド3蛋白質の構造と機能に関する研究" 福岡医学雑誌. 85. 335-340 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ohkita,Y.J.: "Changes in structure of the chromophore in the photochemical process of bovine rhodopsin as revealed by FTIR spectroscopy for hydrogen-out-of-plane vibrations." Biophys.Chem.(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Cao,Y.: "Relationship of proton release at the extracellular surface to deprotonation of the Schiff base in the bacteriorhodopsin photocycle." Biophys.J.(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kandori,H.: "Water-mediated proton transfer in proteins:An FTIR study of bacteriorhodopsin." J.Am.Chem.Soc.(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sasaki,J.: "Nanosecond time-resolved infrared spectroscopy distinguishes two K species in the bacteriorhodopsin photocycle." Biophys.J.(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hara,Y.: "The Sodium Pump" Molecular cloning of P-type ATPases on intracellular membranes of the marine alga Heterosigma akashiwo, 906 (1994)

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      1994 Annual Research Report
  • [Publications] 乾賢一: "膜学実験シリーズ第1巻 生体膜編、日本膜学会編" 共立出版, 424 (1994)

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      1994 Annual Research Report
  • [Publications] 山登一郎: "薬学研究の進歩10巻" 薬学研究奨励財団, (1994)

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      1994 Annual Research Report
  • [Publications] A.Suenaga: "Progress in Cell Research:Molecular Biophysics of Ion Channels(in press)" Elsevier Sci.Pub., (1995)

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      1994 Annual Research Report
  • [Publications] Yamashita,T.: "Characterization of the Na^+/H^+-antiporter of bovine renal cortex and its immunoaffiruity purification in a reconstitutively active form" J.Biochem.113. 136-140 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Suzuki,T.: "Uncompling of ATP splitting from Ca^<2+>-transport in Ca^<2+>-transporting ATPase of the Sarcoplasmic Reticulum as a result of modification by N-(3-pyrene) maleimide:activation of a channel with a specificity for alkaline earth metal ions" J.Biochem.114. 203-209 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kuroda,M.: "Calcium pump expresion in human bone and soft tissue tumors" Virehows Arehiv A Pathol.Anat.423. 209-213 (1993)

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      1993 Annual Research Report
  • [Publications] Hara,T.: "The UDP-galactosetranslocator gene is mapped to the Wiscott-Aldrich syndrome locus of the X chromosome" Somat.Cell Mol.Genet. (in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamamoto,K.: "Regulation of Na,K-adenosine triphospjatase gene sxpression by sodium ioms in cultured neonatal rat cardiocytes" J.Clinical Investigations. 92. 1889-1895 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Aoshima,H.: "Heterologans expression of the H^+/hexose cotransporter from chrorella in xenopus oocytes and its characterization" J.Plant Physiol.141. 293-297 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sumimoto,H: "Role of Src homology 3(SH3)domains in assembly and activation of the phagacyte NADPH oxidase" Proc.Natl.Acad.Sci.USA. (in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] Sumimoto,H: "Formation of a nonvel 20-hydroxylated metablite of lipoxin A_4 by human neutrophil microsomes" FEBS Lett.315. 205-210 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Mizukami,Y: "w-Hydroxylation of lipoxin B_4 by human neutrophil microsomes:identification of w-hydroxy metabolite of lipoxin B_4 and catalysis by leukotriene B_4 w-hydroxylase (cytochrome P-450 LTBw)" Biochim.Biophys.Acta.1168. 87-93 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yu,L: "Characterization of the GTP-dependent activation of the superoxide-producing NADPH oxidase in cell-free system of pig neutrophils" Biochim.Biophys.Acta.1178. 73-80 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Mitsuyama,T: "Cyclic AMP inhibits the respiratory burst of electropermeabilized human neutrophils at a downstream site of protein kinase C" Biochim.Biophys.Acta.1177. 167-173 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kansha,M: "Decrease in the phosphotyrosine phosphatase activity in the plasma membrane of human neutrophils on stimulation by phorbol 12-myristate 13-acetate" Biochim.Biophys.Acta.1179. 189-196 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Saito: "Dipeptide transporters in apical and basolateral membranes of the human intestinal cell line CaCo-2" Am.J.Physiol.265. G289-G294 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Saito: "Expression of human intestinal dipeptide transporter in xenopus laevis occytes" Biochem.Pharmacol. 45. 776-779 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] R.Hori: "Transport of bestatin in rat renal brush-border membrane vesicles" Biochem.Pharmacol.45. 1763-1768 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Ohoka: "p-Aminohippurate transport in rat renal brush-border membranes:a potential-sensitive transport system and an anion exchanger" Biol.Pharm.Bull.16. 395-401 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Katsura: "Characteristics of organic cation transporter in rat renal basolateral membrane" Biochim.Biophys.Acta. 1146. 197-202 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] S.Maeda: "Transport of organic cation in renal brush-border membrane from rats with renal ischemic injury" Biochim.Biophys.Acta. 1150. 103-110 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Bertrand,B.et al.: "The Na^+/H^+ exchanger isoform 1 (NHE1) is a novel member of calmodulin binding proteins." J.Biol.Chem.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Wakabayashi,S.et al.: "Mutaion of calmodulin-binding site renders the Na^+/H^+ exchanger (NHE1) highly H^+-sensitive and Ca^<2+> regulation-defective." J.Biol.Chem.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Wakabayashi,S.et al.: "Growth factor activation and "H^+-sensing" of the Na^+/H^+ exchanger isoform 1 (NHE1).Evidence for an additional mechanism not requirin direct phosphoryla" J.Biol.Chem.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nakasaki,Y.et al.: "Cloning of the rat aortic smooth muscle Na/Ca exchanger and tissue-specific expression of isoforms." J.Biochem.114. 528-534 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Taniguchi,J.et al.: "Maxi K^+ channels are stimulated by cyclic guanosine monophosphate-dependent protein kinase in canine coronary artery smooth muscle cell" Pflugers Arch.423. 167-172 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tawada-Iwata,Y.et al.: "Invreased mechanical extraction of T-tuble/junctional SR from cardiomyopathic hamster heart." Am.J.Physiol.264. H1447-H1453 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamaguchi,A.et al.: "Metal-Tetracycline/H^+ Antiporter of Escherichia coli Encoded by Transposon Tn10:The Structural......" J.Biol.Chem.268. 6496-6504 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamaguchi,A.et al.: "Role of the Conserved Quartet of Residues Located in the N- and C-Terminal Halves of the Transposon Tn10.." Biochemistry. 32. 5698-5704 (1993)

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      1993 Annual Research Report
  • [Publications] Yamaguchi,A.et al.: "The in vivo Assembly of Function of the N- and C-Ter-minal Halves of the Tn10-Encoded TetA protein" FEBS Letters. 324. 131-135 (1993)

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      1993 Annual Research Report
  • [Publications] Yamaguchi,A.et al.: "Effects of Sulfhydryl Reagents on the Cys65 Mutant of the Trans-poson Tn10-Encoded Metal-Tetracyclin/H^+ Antiporter.of E.coli" FEBS Letters. 322. 201-204 (1993)

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      1993 Annual Research Report
  • [Publications] Yamaguchi,A.et al.: "Second-Site Mutation of Ala-220 to Glu or Asp Suppresses the Mutation of Asp-285 to Asn in the Transposon Tn10-Encoded Metal.." J.Biol.Chem.268. 26990-26995 (1993)

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      1993 Annual Research Report
  • [Publications] Yamaguchi,A.et al.: "Hot-Spots for Sulfhydryl Inactivation of Cys Mutants in the Widely Conserved Sequence Motifs of the Metal-Tetracycline/H^+..." J.Biochemistry. 115(in press). (1994)

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      1993 Annual Research Report
  • [Publications] Hara,Y.: "Kinetic analysis and voltage dependency of alpha-3 Na^+/K^+-pump in proceedimgs for the 7th international conference on Na pump" Dr.D Steinkopff Verlag,Darmstadt (in print),

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      1993 Annual Research Report
  • [Publications] 青島 均: 朝倉書店 (井村 裕夫,岡 哲雄,芳賀 達也,岩本 英爾 編)27HC02:レセプター -基礎と臨床 中.ニコチン性アセチルコリンレセプター, 944 pp.400-405 (1993)

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      1993 Annual Research Report
  • [Publications] Takeshige,K: "Active Oxygens,Lipid Peroxides,and Antioxidants" Japan Sci.Soc.Press, 13 (1993)

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      1993 Annual Research Report
  • [Publications] 乾 賢一: "膜学実験シリーズ 第I 巻 生体膜編 日本膜学会編" 共立出版, 424 (1994)

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      1993 Annual Research Report
  • [Publications] 橋本 一,井上 松久 編: "病原菌の薬剤耐性機構の解明とその対策(共著)" 学会出版センター, 198 (1993)

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      1993 Annual Research Report
  • [Publications] 橋本 一,井上 松久 編: "病原菌の薬剤耐性機構の解明とその対策(共著)" 学会出版センター, 198 (1993)(994)

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      1993 Annual Research Report
  • [Publications] T.Yamashita: "Reconstitution of Na^+/H^+-antiporper of bovine renal brush border membrane into proteoliposomes and detection of a 110 KDa protein cross-reactive with antibodies against a human Na^+/H^+-antiporter partial pepride in antiport-active fractions after partial fracti onation" Journal of Biochemisry. 111. 162-167 (1992)

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      1992 Annual Research Report
  • [Publications] H.Hayashi: "Properties of active magnesium flux across the small intestine of the guinea pig" Japanese Journal of Physiology. 42. 561-575 (1992)

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      1992 Annual Research Report
  • [Publications] T.Kitazono: "Activation of cultured bovine aortic endothelial cells by extracellular pyrimidine triphosphate" International Journal of Biochemistry. 24. 1323-1327 (1992)

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      1992 Annual Research Report
  • [Publications] H.Aoshima: "Syntgesis of photolabile caged amino acids for measuring amino acid transporters" Bioscience Biotechnology and Biochemistry. 56. 1086-1089 (1992)

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      1992 Annual Research Report
  • [Publications] A.Yoshida: "Phosphorylation of ryanodine receptor in rat myocytes suring β-adrenargic stimulation" Journal of Biochemistry. 111. 1094-1100 (1992)

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      1992 Annual Research Report
  • [Publications] A.Yanaguchi: "Metal-tetracycline/H^+ antiporter of Esherichia coli encoded by transposon Tn10:Roles of aspartyl residues in the putative transmembrane gelices" Journal of Biological Chemistry. 267. 7409-7418 (1992)

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      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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