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エイズ制圧に向けての基礎研究 柱1 HIVの分子遺伝学

Research Project

Project/Area Number 04269104
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionKyoto University (1993-1994)
Osaka University (1992)

Principal Investigator

畑中 正一 (1993-1994)  京都大学, ウイルス研究所, 教授 (30142300)

中田 篤男 (1992)  大阪大学, 微生物病研究所, 教授 (80029769)

Co-Investigator(Kenkyū-buntansha) 牧 正敏  名古屋大学, 農学部, 教授 (40183610)
塩田 達雄  東京大学, 医科学研究所, 助手 (00187329)
志田 壽利  京都大学, ウイルス研究所, 助教授 (00144395)
足立 昭夫  京都大学, ウイルス研究所, 助教授 (90127043)
品川 日出夫  大阪大学, 微生物病研究所, 教授 (40029799)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥142,400,000 (Direct Cost: ¥142,400,000)
Fiscal Year 1994: ¥58,000,000 (Direct Cost: ¥58,000,000)
Fiscal Year 1993: ¥46,500,000 (Direct Cost: ¥46,500,000)
Fiscal Year 1992: ¥37,900,000 (Direct Cost: ¥37,900,000)
KeywordsHIV-1 / Gag蛋白 / 感染初期 / EnV / Rev / eIF5A / V3領域 / 細胞宿主域 / Env / HIV-1の分子遺伝学 / Gagタンパク質と抗体検出 / Vifとウイルス粒子の成熟促進 / Vpuとウイルス粒子の放出促進 / ワクシニア・ベクターとenvの発現 / Envタンパク質の抗原性 / RNA-PCR法によるV3領域の増幅 / V3領域の変異と宿主域の拡大
Research Abstract

大腸菌で産生させ精製したHIV-1のGag先駆体p55は、リコンビナントGag(308-406)-Env(512-611)キメラ蛋白や逆転写酵素と同等の反応性を有する優れた高原であった。これらを用いた高感度診断法を確立した。HIV-2のGag蛋白p26と逆転写酵素の多量生産と精製に成功した(品川)。
HIV-1とHIV-2には合計5種類のアクセサリー遺伝子がある。我々の開発したシステムによる解析から、これらの遺伝子産物が効果をあらわす時期は、Vif,Vpr,Vpr,Nefが感染初期であり、Vpuが感染後期であることがわかった。また、Gag変異体の解析からGagの機能が感染初期と後期の両方に要求されることも判明した(足立)。
組換えワクシニアを用いてEnvとCD4により引き起こされる細胞融合を鋭敏に検出する方法を開発した。β-ガラクトシダーゼのαとω相補を利用するものである。この方法によりサイトカラシンDの阻害効果と、融合に関与する細胞成分の存在を示した(志田)。
HIV感染者の体内のHIV-のEnv遺伝子V1領域からV3領域の構造を経時的に解析した。病態の進行した症例では、V3には塩基性荷電が蓄積し、V2には新たな糖鎖付加部位の挿入が認められた。V2の糖鎖付加部位の挿入はV3の塩基性荷電が未だ増加していない症例でも認められた(塩田)。
AIDS遺伝子治療に関する基礎的研究として、HIV-1増殖に必須の調節因子Revタンパク質の機能を強力に抑制するRev変異体を作製し、in vitroにおけるこの変異体の抗ウイルス作用を解析した。Rev変異体遺伝子を恒常的に導入したヒト培養細胞ではHIV-1の増殖ならびに細胞障害性が顕著に抑制された(牧、畑中)。

Report

(3 results)
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (90 results)

All Other

All Publications (90 results)

  • [Publications] Kubota, S.: "The origin of human immunodeficiency virus type-1 rev gene:An evolutionary hypothesis" FEBS Lett.338. 118-121 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ueba, T: "Expression of fibroblast growth factor receptor-1 in human glioma and menigioma tissues" Neurosurgery. 34. 221-226 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] El-Farrash, M.A.: "Generation and characterization of a human immunodeficiency virus type 1(HIV-1) mutant resistant to an HIV--1 protease inhibitor" J. Virol.68. 233-239 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamada, T.: "The human T-cell leukemia virus type I Tax protein induces apoptosis which is blocked by the Bcl-2 protein" J. Virol.68. 3374-3379 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yang,H.Q.: "Analysis of calcium-dependent interaction between amino-terminal conserved region of calpastatin functional domain and calmodulin-like domain of μ-calpain large subunit" J. Biol. Chem.269. 18977-18984 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Suzui, H.: "Immunohistochemical reactions for fibroblast growth factor receptor in arteries of patients with moyamoya disease" Neurosurgery. 35. 20-25 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Suzui, H.: "Immunohistochemical study for basic fibroblast growth factor receptor I in pituitary adenomas" Neurosci, Lett.171. 192-196 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ueba, T.: "Transcriptional regulation of basic fibroblast grwoth factor gene by p53 in human glioblastoma and hepatocellular carcinoma cells" Proc. Natl. Acad. Sci. USA. 91. 9009-9013 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takano, E.: "Real-time analysis of the calcium-dependent interaction between calmodulin and a synthetic oligopeptide of calcineurin by a surface plasmon resonance biosensor" FEBS Lett.352. 247-250 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ma, H.: "Amino-terminal conserved region in proteinase inhibitory activity by interacting with calmodulin-like domain of the proteinase" J. Biol. Chem.269. 24430-24436 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Satoh, T.: "Histamine as an activator of cell growth and extracellular matrix reconstruction for human vascular smooth muscle cells" Atherosclerosis. 110. 53-61 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Inoue, H.: "Suppression of the transformed phenotype in hybrids of human T-cell leukemia virus type I tax-transformed rat fibroblasts and normal human fibroblasts" Exp. Cell Res.215. 68-74 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamaguchi, R.: "Unphosphorylated and tyrosine phosphorylated forms of a focal adhesion protein,paxillin,are substrates for calpain II in vitro:implications for the possible involvement of calpain II in mitosis-specific degradation of paxillin" FEBS Lett.356. 114-116 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Miyamoto, S.: "Tumor necrosis factor α-induced phosphorylation of IκBα is a signal for its degradation but not dissociation from NF-κB" Proc. Natl. Acad. Sci. USA. 91. 12740-12744 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Iwakura, Y.: "Augmentation of c-fos and c-jun expression in transgenic mice carrying the human T-cell leukemia virus type-I tax gene" Virus Gene. 9. 161-170 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hirota, K.: "Sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for antibodies to reverse transcriptase(RT) of human immunodeficiency virus type 1(HIV-1)using recombinant RT of HIV-1 as antigen" Clin. Chem. Enzym. Comms.6. 175-184 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hashinaka, K.: "Detection of anti-human immunodeficiency virus type1(HIV-1)immunoglobulin G in urine by an ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) with recombinant reverse transcriptase as an antigen" J. Clin. Microbiol.32. 819-822 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hashida, S.: "Detection of antibody IgG to HIV-1 in urine by ultrasensitive enzyme immunoassay (immune complex tranfer eyzyme immunoassay)using recombinant p24as antigne for diagnosis of HIV-1 infection" J. Clin. Lab. Anal.8. 86-95 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hashida, S.: "Diagnosis of HIV-1 infection by detection of antibody IgG to HIV-1 in urine with ultrasensitive enzyme immunoassay(immune complex transfer enzyme immunoassay)using recombinant proteins as antigens" J. Clin. Lab. Anal.8. 237-246 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hashinaka, K.: "Conjugation of recombinant reverse transcriptase of HIV-1 to β-D-galactosidase from Escherichia coli for ultrasensitive enzyme immunoassay(immune complex transfer enzyme immunoassay of anti-HIV-1 IgG" J. Immunol. Meth.172. 179-187 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nakagawa, Y.: "Inhibition of murine AIDS(MAIDS)development by the transplantation of bone marrow cells carrying the Fv-4 resistant gene to MAIDS virus-infected mice" J. Virol.68. 1438-1441 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kawamura, M.: "Growth ability of human immunodeficiency virus type 1 auxiliary gene mutants in primary blood macrophage cultures" J. Gen. Virol.75. 2427-2431 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Igarashi, T.: "Persistent infection with SIVmac chimeric virus having tat,rev vpu,env,and nef of HIV-1 in macaque Pmonkeys" AIDS Res. Human Retroviruses. 10. 1021-1029 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kawamura, M.: "Human immunodeficiency virus Vpx is required for the early phase of replication in peripheral blood mononuclear cells" Microbiol. Immunol.38. 871-878 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Katahira, J.: "Effect of translation initiation factor elF-5A on HTLV-1 Rex and HIV Rev functions that had been inhibited trans-dominantly a Rex mutant deficient in RNA binding" J. Virol.(in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Adachi, A..: "Methods for HIV-SIV gene analysis" Methods Mol. Genet.(in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Kannagi, M.: "Cytotoxic T cell response and expression of the target antigen in HTLV-1 infection" Leukemia. 8. 54-59 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Jin, N-Y.: "Nigh level of expression by vaccinia virus vectors based on combination effect of promoters of A-type inclusion body,tandemly repeated mutant 7.5-KDa protein and hemagglutinin genes" Arch. Virol.138. 315-330 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tanaka, Y.: "Induction of antibody responses that neutralize human T-cell leukemia virus type I infection in vitro and in vivo by peptide immunization" J. Virol.68. 6323-6331 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Katahira, J.: "A cellular protein which associates with HTLV-1 Rex protein in the presence of the target mRNA" Oncogene. 9. 3535-3544 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Furukawa, K.: "Clonal expansion of CD8+ cytotoxic T lymphocytes against human T cell lymphotropic virus type I (HTLV-1)genome products in HTLV-1 assoiciated myelopathy/tropical spastic paraparesis patients" J. Clin. Invest.94. 1830-1839 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ohnishi, Y.: "A furin-defective cell line is able to process correctly the gp160 of human immunodeficiency virus type 1" J. Virol.68. 4075-4079 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Oka, S.: "Genetic analysis of HTV-1 during rapid progression to AIDS in an apparently healthy man" AIDS Res. Human Retroviruses. 10. 271-277 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shinoda, T.: "A naturally occurring single basic amino acid substitution in the V3 region of the human immunodeficiency virus type 1 Env protein alters the cellular host range and antigenic structure of the virus" J. Virol.68. 7689-769619GA35:Furuta, R.A. (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] "Use of a human immunodeficiency virus type 1 Rev mutant without nucleolar dysfunction as a candidate for potential AIDS therapy" J. Virol.69. 1591-1599 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 畑中 正一: "ATLとAIDS" 南江堂, 150 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Gordon, S.: "The Legacy of Cell Fusion" Oxford Science Publications, 295 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nam,S.H.: "Characterization of ribosomal frameshifting for expression of pol gene products of human T-cell leukemia virus type I" J.Virol.67. 196-203 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takahashi,J.B.: "Developmental expression of trk B and low-affinity NGF receptor in the rat retina" Neurosci.Lett.151. 174-177 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takano,E.: "Molecular diversity of calpastatin in human erythroid cells" Arch.Biochem.Biophys.303. 349-354 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Adachi,Y.: "Nucleolar targeting signal of Rex protein of human T-cell leukemi virus type I specifically binds to nucleolar shuttle protein B-23" J.Biol.Chem.268. 13930-13934 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nakajima,T.: "Overgrowth of human synoviral cells driven by the human T cell leukemia virus type I tax gene" J.Clin.Invest.92. 186-193 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kubota,S.: "Long cellular repeats flanking a defective HTLV-I provirus:implication for site-targeted integration" Oncogene. 8. 2873-2877 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nosaka,T.: "Cytotoxic activity of rev protein of human immunodeficiency virys type I by nucleolar dysfunction" Exp.Cell Res.209. 89-102 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nosaka,T.: "Novel internal promoter/enhancer of HTLV-I for tax expression" Nucleic Acids Res.21. 5124-5129 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hatanaka,M.: "Nucleolar targeting signal" J.UOEH. 15. 167-197 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kubota,S.: "The origin of human immunodeficiency virus type I rev gene:An evolutionary hypothesis" FEBS Lett.338. 118-121 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ueba,T.: "Expression of fibroblast growth factor receptor-1 in human glioma and meningioma tissues" Neurosurgery. 34. 221-226 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] El-Farrash,M.A.: "Generation and characterization of a human immunodeficiency virus type 1(HIV-1)mutant resistant to an HIV-1 protease inhibitor" J.Virol.68. 233-239 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Morimoto,M.: "Use of the reconbinat chimera proteins,LacZ-Env and Gag-Env,for immunological studies on HIV-1 infection" AIDS Res.Hum.Retroviruses. 9. 971-978 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hashida,S.: "Detection of antibody IgG to HIV-1 in urine by sensitive enzyme immunoassay(immune complex transfer enzyme immunoassay)using recombinant protein as antigens for diagnosis of HIV-1 infection" J.Clin.Lab.Anal.7. 353-364 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hirota,S.: "Sensitive enzyme immunoassay(immune complex transfer enzyme immunoassay)for antibodies to reverse transcriptase(RT)of human immunodeficiency virus type I(HIV-I)using recombinant RT of HIV-I as antigen" Clin.Chem.Enzym.Comms.(in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] Hashida,S.: "Anti-HIV-I IgG in urine detected by sensitive enzyme immunoassay(immune complex transfer enzyme immunoassay)using recombinant proteins as antigens for diagnosis of HIV-I infection" Clin.Chem.Enzym.Comms.(in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] Miyazawa,T.: "The AP-1 binding site in the feline immunodeficiency virus long terminal repeat is not required for virus replication in feline T lymphocytes" J.Gen.Virol.74. 1537-1580 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sakai,H.: "Integration is essential for efficient gene expression of human immunodeficiency virus type 1" J.Virol.67. 1169-1174 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sakai,H.: "Cell-dependent requirement of human immunodeficiency virus type 1 Vif protein for maturation of virus particles" J.Virol.67. 1663-1666 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sakai,H.: "Compatibility of tat and rev transactivators in the primate lentiviruses" Arch.Virol.129. 1-10 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tomonaga,K.: "The feline immunodeficiency virus ORF-A gene facilitates efficient viral replication in established T-cell lines and peripheral blood lymphocytes" J.Virol.67. 5889-5895 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tomonaga,K.: "Feline immunodeficiency virus gene expression:analysis of the RNA splicing pattern and monocistronic rev mRNA" J.Gen.Virol.74. 2409-2417 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Schmelz,M.: "Assembly of vaccinia virus:the second wrapping cisterna is derived from the trans Golgi network" J.Virol.68. 130-147 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kannagi,M.: "Cytotoxic T cell response and expression of the target antigen in HILV-I infection" Leukemia. (in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] LeGuern,M.: "Single amino acid change in Tat determine the different rates replication of two sequential HIV-1 isolates" Virology. 195. 441-447 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Oka,S.: "Genetic analysis of HIV-1 during rapid progression to AIDS in an apparently healthy man" AIDS Res.Hum.Retroviruses. (in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] Hatanaka,M.: "Biology of the Germ Line" Japn Sci.Soc.Press,Karger, 304 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hatanaka,M.: "Molecular Basis of Immune Responses" Academic Press Inc., 287 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 畑中正一: "ATLとAIDS" 南江堂, 150 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 畑中正一: "ウイルスとの闘い:がん・エイズは克服できるか" NHK出版協会, 128 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 川村名子: "エイズ研究の最先端(分担執筆:HIVの制御遺伝子と遺伝子治療)" 羊土社, 166 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 川村名子: "HIV感染症・AIDS(分担執筆:HIV遺伝子の構造と機能)" 日本臨牀社, 658 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 志田壽利: "エイズ研究の最先端(基礎から臨床へのup-to-dateな分子医学)" 羊土社, 166 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Saitoh,A.,Tanaka,N.,Nakata,A.,Ikuta,K.and Shinagawa,H.: "A Unique Monoclonal Antibody That Recognizes Mature o17 of HIV-1 but Not Its Precursor" Microbiol.Immunol.36. 105-111 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tanaka,N.,Saitoh,A.,Nakata,A.and Shinagawa H.: "A Sinple Method for Overproduction and Purification of p24 Gag Protein of Human Immunodeficiency Virus Type 1" Microbiol.Immunol.36. 823-831 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sakai,H.,Sakuragi,J.,Sakuragi,S.,Shibata,R.and Adachi,A.: "Functional analysis of biologically distinct genetic variants of simian immunodeficiency virus isolated from a mandrill." Virology. 189. 161-166 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sakuragi,J.,Sakai,H.,Sakurage,S.,Shibata,R.,Wain-Hobson,S.,Hayami,M., and Adachi,A.: "Functional classification of simian immunodeficiency virus isolated from a chimpanzee by transactivators." Virology. 189. 354-358 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sakai,H.,Sakuragi,J.,Sakuragi,S.,Shivata,R.,Hayami,M.,Ishimoto,A.and Adachi,A.: "Genetic characterization of simian immunodeficiency virus esolated from an African mandrill." Arch.Virol.125. 1-14 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tomonaga,K.,Norimine,J.,Shin,Y.S.,Fukasawa,M.,Miyazawa,T.,Adachi,A.,Toyosaki,T.,Kawaguchi Y.,Kai,C.a: "Identification of afeline immunodeficiency virus gene which is essential for cell-free virus infectivity." J.Virol.66. 6181-6185 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sakuragi S,Shibata,R.,Mukai,R.,Komatsu,T.,Fukasawa,M.,Sakai,H.,Sakuragi,J.,Kawamura,M., ibuki,K.,Hayami,M.and Adachi.A.: "Infection of macaque monkeys with a chimeric human and simian immunodeficiency virus." J.Gen.Virol.73. 2983-2987 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sakai,H.,Sakuragi,S.,Sakuragi,J.,Kawamura,M.,Shibata,R.and Adachi,A.: "Sequences responsible for efficient replication of simian immunodeficiency virus SIVmnd in cells of the monocyte/macrophage Iineage." J.Gen.Virol.73. 2989-2993 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sakai,H.,Kawamura,M.,Sakuragi,J.,SAkuragi,S.,Shibata,R.,Ishimoto.A.,Ono,N.,Ueda,S. and Adachi,A.: "Integration is essential for efficient gene expression of human immunodeficiency virus type 1." J.Virol.67. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sakai,H.,SHibata,R.,Sakuragi,J.,Sakuragi,S.,Kawamura,M.and Adachi,A.: "Cell-dependent requirement of human immunodeficiency virus type 1 vif protein for maturation of virus particles." J.Virol.67. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sakai,H.,Sakuragi,J.,Sakuragi,S.,Kawamura,M.and Adachi A.: "Compatibility of tat and rev transactivators in the primate lentiviruses." Arch.Virol.(1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Adachi,A.,Sakuragi,S.,Kawamura,M.and Sakai,H.: "Genetic analysis of primate immunodeficiency viruses." Methods in Molecular Genetics. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Miyazawa,T.,Kohmoto,M.,Kawaguchi,Y.,Tomonaga,K.,Touosaki,T.,Ikuta,K.,Adachi,A.and Mikami,T.: "The AP-1 binding site in the feline immunodeficiency virus long terrninal repeat is not requered for virus replication in feline T lymphocytes." J.Gen.Virol.(1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tanaka,T.,Masuda,M.,Yoshida,A.,Shida,H.,Nyunoya,H.,Shimotohno,K.and Tozawa,H.: "Antigenic structure of the transactivator protein encoded by human T-cell leukemia virus type-1(HTLV-1),as defined by a panel of monoclonal antibodies." AIDS Res.Human Retroviruses. 8. 227-235 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Taguchi,F.,Ikeda,T.and Shida,H.: "Molecular cloning and expression of a spike protein of neurovirulent munne corona virus JHMV variant c1-2." J.Gen.Virol.73. 1065-1072 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kannagi,M.,Shida,H.,Igarashi,H.,Maruyama,I.,Hattori,T.,Osame,M.,Inoko,H.and Harada,S.: "Target epitope in the tax protein of human T cell leukemia virus type I recognized by class I major histocompatibility complex-restricted cytotoxic T cell." J.Virol.66. 2928-2933 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Iwakura,Y.,Shioda,T.,Tosu,M.,Yoshida,E.,Havashi,M.,Nagata,T.,and Shibuta,H.: "The induction of cataracts by HIV-1 in transgenic mice." AIDS. 6. 1069-1075 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Shioda,T.,Levy,J.A.,and Cheng-Mayer,C.: "Small amino acid changes in the V3 hyper-variable region of gp120 can affect T-cell line and macrophage tropisms of HIV-1." Proc.Natl.Acad.Sci.USA. 89. 9434-9438 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hayashi,T.,Shioda,T.,Iwakura,Y.,and Shibuta,H.: "RNA packaging signal of human immunodeficiency virus type 1." Virology. 188. 590-599 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 志田 壽利: "蛋白質核酸酵素" 共立出版, 6 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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