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RNAレプリコン

Research Project

Project/Area Number 04271101
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionThe University of Tokyo

Principal Investigator

野本 明男  東京大学, 医科学研究所, 教授 (70112670)

Co-Investigator(Kenkyū-buntansha) 岡田 吉美  帝京大学, 理工学部, 教授 (30011703)
豊島 久真男  大阪府立成人病センター, 総長 (90029760)
吉倉 廣  東京大学, 医学部, 教授 (60012754)
永井 美之  東京大学, 医科学研究所, 教授 (20022874)
石浜 明  国立遺伝学研究所, 教授 (80019869)
豊田 春香  北里大学, 薬学部, 助手 (10197973)
永田 恭介  東京工業大学, 理工学部, 助教授 (40180492)
水本 清久  北里大学, 薬学部, 教授 (80092344)
Project Period (FY) 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥401,600,000 (Direct Cost: ¥401,600,000)
Fiscal Year 1995: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1994: ¥144,000,000 (Direct Cost: ¥144,000,000)
Fiscal Year 1993: ¥143,100,000 (Direct Cost: ¥143,100,000)
Fiscal Year 1992: ¥111,500,000 (Direct Cost: ¥111,500,000)
KeywordsRNA複製 / 転写 / 翻訳 / 病原性 / ウイルスRNA / ウイルス蛋白質 / 複製 / 宿主分子 / トランスジェニックマウス / RNAレプリコン
Research Abstract

本年度は、重点領域研究「RNAレプリコン」のとりまとめを行った。平成7年6月12日に北里大学において、第1回総括班会議を開催し、とりまとめに関する話し合いが行われた。この会議では、これまでのとりまとめ方法のみならず、今後のRNAレプリコン研究発展を目指した本年度の活動が話し合われた。その結果、「RNA情報のフロンティア」と題する一般公開シンポジウムを11月28、29日の両日、日経ホールにて開催することが決定した。このシンポジウムは重点研究「エイズの病態と制御に関する基礎研究」(代表・永井美之)との合同シンポジウムとし、RNA情報の多元的制御メカニズムを紹介、さらにRNA研究の重要性と面白さを一般にアピールすることであった。
例年のように、各種RNAレプリコン単位のミニシンポジウムも開催することが決定され、植物のRNAレプリコン会議(世話人・渡辺雄一郎)およびレトロウイルス複製会議(世話人・岩本愛吉)が、それぞれ岡山大学資源生物研究所(平成7年10月30日)および東京大学医科学研究所(平成8年1月20日)において開催された。さらに、この領域の若手を世話人(永田恭介、小林信之、中西義信)とした公開シンポジウム「ウイルスを利用する人類の知恵-アポトーシス制御、ウイルスベクター、遺伝子治療」を平成8年2月6日に東京大学山上会館で開催した。いずれのシンポジウムでも多くの参加者が集まり、熱のこもった討論が行われた。
本年度の最後には、ニュースレター最終版を発行し、来年度の前半に領域代表者による研究報告書の作成が行われることが決定されている。

Report

(4 results)
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] S.Abe: "Neurovirulence test for oral live poliovaccines using poliovirus-sensitive transgenic mice" Virology. 206. 1075-1083 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Shiroki: "A new cis-acting element for RNA replication within the 5′noncoding region of poliovirus type 1RNA" J.Virol.69. 6825-6832 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] S.Abe: "Studies on neurovirulence in poliovirus sensitive-transgenic mice and cynomolgus monkeys for the different-" Virology. 210. 160-166 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] B.P.Mahon: "Poliovirus-specific CD4^+ Th1 clones with both cytotoxic and helper activity mediate protective humoral immunity" J.Exp.Med.181. 1285-1292 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Nakagawa: "RNA polymerase PB2 subunit is not required for replication of the influenza virus genome, but is involved in capped RNA synthesis" J.Virol.69. 728-733 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Mizumoto: "Protein factors required for in vitro transcription of Sendai virus genome" J.Biochem.117. 527-534 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] A.Nomoto: "The World Congress on Alternatives and Animal Use in the Life Science" Mary Ann Liebert Inc., 738 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] A.Nomoto: "Hepatitis C virus and its involvement in the development of hepatocellular carcinema" The Princess Takamatsu Cancer Research Fund(in press),

    • Related Report
      1995 Annual Research Report
  • [Publications] Akio Nomoto: "Tissue tropism and species specificity of poliovirus infection" Trends in Microbiology. 2. 47-51 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hitoshi Horie: "Transgenic mice carrying the human poliovirus receptor : new animal model for study of poliovirus nourorirulence" Journal of Virology. 68. 681-688 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Junken Aoki: "Amino ecid residues on human poliovirus receptor involved in interaction with poliovirus" Journal of Biological Chemistry. 269. 8431-8438 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Satoshi Koike: "Characterization of three different transgenic mouse lines that carry human poliovirus receptor gene-Influence of transgene expression on pathogenesis" Archives of Virology. 139. 351-363 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shinobu Abe: "Neurovirulence test for oral live poliovaccines using poliovirus-sensitive transgenic mice" Virology. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Haruka Toyoda: "Host factors required for internal initiation of translation on poliovirus RNA" Archives of Virology. 138. 1-15 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Satoshi Koike: "Cellular Receptors for Animal Viruses" Cold Spring Harbor Laboratory Press, 600 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Akio Nomoto: "Viral Hepatitis and Liver Disease" Springer-Verlag, 802 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kazuko Shiroki: "Temperature sensitive mouse cell factors for strand-specific initiation of poliovirus RNA synthesis" Journal of Virology. 67. 3989-3996 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kyoko Tsukiyama-Kohara: "Antigenicities of group I and group II hepatitis C virus peptides-molecular basis of diagnosis" Virology. 192. 430-437 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hitoshi Horie: "Transgenic mice carrying the human poliovirus receptor:new animal model for study of poliovirus neurovirulence" Journal of Virology. 68. 681-688 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Junken Aoki: "Amino acid residues on human poliovirus receptor involved in interaction with poliovirus" Journal of Biological Chemistry. (in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Akio Nomoto: "Species specificity and tissue tropism of poliovirus infection" Trends in Microbiology. 2. 47-51 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yoshiyuki Nagai: "Protease dependent virus tropism and pathogenicity" Trends in Microbiology. 1. 81-87 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Michinori Kohara: "Measles and Poliomyelitis" Springer-Verlag, (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Satoshi Koike: "Receptor Mediated Virus Entry into Cells" Cold Spring Harbor Laboratory Press, (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Satoshi Koike: "A second gene for African green monkey poliovirus receptor that has no putative N-glycosylation site in the functional N-terminal immunoglobulin-like domain." Journal of Virology. 66. 7059-7066 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yoshio Shibagaki: "mRNA capping enzyme" Journal of Biological Chemistry. 267. 9521-9528 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Kobayashi: "Reconstitution of influenza virus RNA polymersse from three subunits expressed using recombinant baculovious system" Virus Research. 22. 235-245 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Ogasawara: "Expression of factor X and its significance for the determination" EMBO Journal. 11. 467-472 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] A.Naito: "Similarity of the genomic structure between the two mambers in a new family of heparin-binding factors." Biochem.Biophys.Res.Commun.183. 701-707 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Nakayama: "Enzymatic properties of the mouse Mx1 protein-associated GTPase" Virus Reserach. 22. 227-234 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Akio Nomoto: "Molecular Neurovirology" Humana Press, 597 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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