| Project/Area Number |
04304007
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
動物発生・生理学
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
SHIMADA Hiraku Faculty of Science, Hiroshima University.Professor, 理学部, 教授 (70011559)
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| Co-Investigator(Kenkyū-buntansha) |
SUEMITSU Takashi Fac.Sci., Saitama Univ., Asso.Prof., 理学部, 助教授 (40092019)
SATOH Noriyuki Fac.Sci., Kyoto Univ., Professor, 理学部, 教授 (30025481)
ABE Shinichi Fac.Sci., Kumamoto Univ., Professor, 理学部, 教授 (90109637)
YAMAGAMI Kenjiro Fac.Sci., Sophia Univ.Professor, 理工学部, 教授 (50011474)
KATAGIRI Chiaki Fac.Sci., Hokkaido Univ., Professor, 理学研究科, 教授 (90000827)
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥19,000,000 (Direct Cost: ¥19,000,000)
Fiscal Year 1994: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1993: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1992: ¥9,000,000 (Direct Cost: ¥9,000,000)
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| Keywords | gene expression / developmet / amphibia / fish / ascidia / sea urchin / artemia / planaria / 発生 / 遺伝子クローニング / 遺伝子発現調節 / 分化マーカー / 多様性 |
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| Research Abstract |
A recent remarkable accumulation of information on gene regulation of various events during early development of animals has been obtained through studies using only limitted variety of animal species, such as some species of mammals, amphibians, an insect (Drosophila) and a nematoda (C.elegans). However, for an extensive understanding of molecular processes governing early development, the detailed information on the regulation of gene expression during embryogenesis of a large variety of animals is definitely expected. The present research project has thus been proposed to access the comparative aspect of gene regulation of early development. The following studies were performed in this project. (1) The collagenase gene was cloned from the frog (Rana catesbiana) and its expression in larval skin was studied in relation to metamorphosis. (2) A cDNA,expression of which changes markedly during metamorphosis, was cloned from liver of Xenopus laevis tadpole. (3) A trial to detect sperm-specific genes from spermatogonia of a newt (Triturus pyrrhogaster). (4)Genes for the fish (Oryzias latipes) hatching enzyme were cloned and their expression in prehatching stages was studied. (5) Transcription factors regulating the skeletal muscle actin genes in embryos of a solitary ascidian (Halocynthia roretzi) were studied. (6) LIM-class homeotic genes were cloned from Halocynthia roretzi and pattern of their expression during development was studied. (7) Genes related to budding of a colonial ascidian (Polyandrocarpa misakiensis) were cloned. (8) Regulation of genes for arylsulfatase in the sea urchin (Hemicentrotus pulcherrimus) and for the exogastrula-inducing peptides of the sea urchin (Anthocidaris crassispina) were studied. (8) Cloning of the treharase cDNA was started in relation to crustacian dormancy (Artemia salina). (9) An extensive search for genes related to regulation of planarian (Dugesia japonica) regeneration was started.
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