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Development of biological engineering for plant breeding of next generation

Research Project

Project/Area Number 04304011
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field Breeding science
Research InstitutionThe University of Tokyo

Principal Investigator

TAKEDA Genkichi  University of Tokyo, Dep.of Agriculture, Professor, 農学部, 教授 (90134501)

Co-Investigator(Kenkyū-buntansha) HINATA Koukichi  Tohoku university, Dep.of Agriculture, Professor, 農学部, 教授 (00005589)
KINOSHITA Toshiro  Hokkaido university, Dep.of Agriculture, Professor, 農学部, 教授 (10001421)
UKAI Yasuo  University of Tokyo, Dep.of Agriculture, Professor, 農学部, 教授 (80240729)
ADACHI Yasusi  Miyazaki university, Dep.of Agriculture, Professor, 農学部, 教授 (50040861)
HIRAI Atsushi  University of Tokyo, Dep.of Agriculture, Professor, 農学部, 教授 (60023470)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥18,000,000 (Direct Cost: ¥18,000,000)
Fiscal Year 1994: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1993: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1992: ¥9,000,000 (Direct Cost: ¥9,000,000)
KeywordsBiological engineering / Advanced technology of Breeding / Somatic hybridization / Insect resistance / Apomixis / Chloroplast genome / Disease resistance / Genetic resources / 葉緑体ゲノム
Research Abstract

This study aimed to develop new breeding technology which was applicable to the breeding in the next century. With this in view, advanced studies of biological engineering were selected from the field of plant breeding.
In the field of cytological engineering, cytoplasmic male sterility were efficiently produced in rice by asymmetric somatic hybridization, and a physical map and a transcriptional map of mitochondrial genome were made in sugar beet(KINOSHITA). Transcriptional maps of chroloplast and of mitochondria were completed in rice based on cone bank which covers whole genome of mitochondria(HIRAI).
In the field of DNA polymorphism utilization, analysis theory for mapping of quantitative trait was developed, and computer program which worked on personal computer, was written based on this theory(UKAI). RAPD and DNA markers were applied for selection of disease resistance in Tomato(NISHIMURA)and of insect resistance in rice(KANEDA). slg which made grain slender and activate mutator i … More n rice was investigated by RFLP(TANISAKA).
In the field of new technology for chromosome mapping, fluorescence in situ hybridization and gimasa method were applied for chromosome mapping of rice. Fluorescence differential staining and scanning electron microscopy were supposed to be effective(FUTSHARA).
In the field of reproduction, DNA sequences of SLG,SRK,SRA and SRB those related to homomorphic selfincompatibility of Brassica were compared each other.While carambola was investigate as a model of heteromorphic self-incompatibility(HINATA). Apomixis were studied for fixation of heterosis(ADACHI). Moreover, followings were studied, improvement of in vitro pollination, introduction of artificially constructed chimeric genes into pollen by polycation and analysis of proteins those secreted in pollen tube pathway(TAKEDA).
In the field of obtaining useful gene or strain from genetic resources, it was revealed that genetic variability of disease resistance in wild rice population was larger than in cultivated rice population(MORISHIMA). While chromosome additional line were produced by introducing a part of chromosome of O.punctata into cultivated rice(IWATA). Less

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Kubo,T.et al: "The sugar but mitochondrial genome contuins an ORF sharing sequence homology with the gene for the 30kDa subunit of borine mitolhondrial compsex I." Mol.Gen.Genet.241. 479-482 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Watanabe,M et al: "A high degree of homology exists between the protein encoded by SLG and the S receptor domain encoded by SRK in self-incompatible Brassica campestris L." Plant Cell Physiol.35. 1221-1229 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hayashi,T.et al: "Change in genetic variame under selection in a self-fertiliging population." G enetics. 136. 693-704 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] A kashi,R and T.Adachi: "Plant regeneration from seed-derived embryogenu callus and all suspension cultures of bahi a grass(Paspalum notatum)." Plant Sci.90. 73-80 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nakazono,M at al: "Palindromic repeated sebuences(PRSs)in the mitochondrial genome of rice, evidcnce for their insertion after divergence of the genus Oryga from the other Gramineae." Plant Mol Biol.24. 273-281 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshida,K.T.et al: "cDNA cloning of regeneration-specific genes in rice by differential screening of randomly amplified cDNAs using RAPD primess." Plant Cell Phsysiol.35. 1003-1009 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kubo, T.et al.: "The sugarbeet mitochondrial genome contains an ORF sharing sequence homology with the gene for the 30 KDa subunit of bovine mitochondrial complex I." Mol.Gen.Genet.241. 479-482 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Watanabe, M.et al.: "A high degree of homology exists between the protein encoded by SLG and the S receptor domatin encoded by SRK in self-incompatibile Brassica campestris L." Plant Cell Physiol.35. 1221-1229 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hayashi, T.and Y.Ukai :"Change in genetic variance under selection in a sefl-fertilizing population." Genetics. 136. 693-704 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Akashi, R.and T.Adachi :"Plant regeneration from suspension cultured-derived protoplasts of apomictic dallisgrass(Paspalum dilatatum Poir.)." Plant Sci.82. 219-225 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nakazono, M., A.Kanno, N.Tsutsumi and A.Hirai :"Palindromic repeated sequences(PRSs)in the mitochondrial genome of rice : evidence for their insertion after divergence of the genus Oryza from the other Gramineae." Plant Mol.Biol.24. 273-281 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshida, K.T., S.Naito and G.Takeda :"DNA cloning of regeneration-specific genes in rice by differential screening of randomly amplified cDNAs using RAPD primers." Plant Cell Physiol.35. 1003-1009 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kubo,T.et al: "The sugar beet mitochondrial genome contains an ORF sharing sequence homology with the gene for the 30kDa subunit of bovine mitochondrial complex I." Mol.Gen.Genet.241. 479-482 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Watanabe,M.et al: "A high degree of homology exists between the protein encoded by SLG and the S receptor domain encoded by SRK in self-incompatible Brassica campestris I." Plant Cell Physiol.35. 1221-1229 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hayashi,T.et al: "Change in genetic variance under selection in a self-fertilizing population." Genetics. 136. 693-704 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Akashi,R.and T.Adachi: "Plant regeneration from seed-derived embryogenic callus and cell suspension cultures of bahiagrass(Paspalum notatum)." Plant Sci.90. 73-80 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nakazono,M.et al: "Palindromic repeated sequances(PRSs)in the mitochondrial genome of rice.evidence for their insertion after divergence of the genus Dryra from the other Gramineae." Plant Mol.Biol.24. 273-281 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yoshida,KT.et al: "cDNA choning of regeneration-specific genes in rice by differential screening of randomly conylified cDNAs using RAPD primess." Plant cell physiol.35. 1003-1009 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Nakazaki: "RFLP analysis for anthosyanin pigmentation gene of a mutant line in rice" Proc.of the 7th Internatl.Congr.of SABRAO. (in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] A.Kanno: "A transcription map of the chloroplast genome from rice (Oryza sativa)" Curr.Genet.23. 166-174 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Nakazono: "Identification of the entire set of transferred chloroplast DNA sequences in the mitochondrial genome of rice" Mol.Gen.Genet.236. 341-346 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] C.Tsukamoto: "Chemotaxonomic markers in Brassica seeds at the species and subspecies levels" Biosci.Biotech.Biochem.57. 653-654 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] I.S.Nou: "Isolation of S-alleles from a wild population of Brassica campestris L.at Balcesme,Turkey and their characterization by S-glycoprs-teins" Sex.Plant Reprod.6. 71-78 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Koboyama: "The diversity of interspecific pollen-pistil incongruity in Nicotiaria" Sex.Plant Reprod.(in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] L.Chen: "Embryo aborion and efficient rescue in interspecific hybrid,Lycopersicon esculentum and peruvianum-complex." Japan.J.Breed.42. 65-77 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Yoshida: "Morphoganetic Pathway specific proteins in rice callus." Plent Tissue Culture and Gene Manipulation for Breeding and Formation of Phytochemicals(ed.by.K.Oono etal. 133-142 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] A.B.Mendoza: "Histological observatons on plent Regeneration in rice(Oryza sativa L.)calli." Japan.J.Breed.42. 33-41 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Li: "Changos in isozyme pattern associated with development of borkey calli derived from matura embryo culture of barley." Japan.J.Breed.42. 535-543 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Kishima: "Conparative analysis of petal proteins in red and white ines from neer-isoganic Portulaca sp.(Jewel)plants." Euphytica. 61. 67-71 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] A.Kanno: "Comparative studies on the structure of chloroplast DNA from bour species of Oryza:CLOning and physical map." Theor.Appl.Cenet.83. 791-798 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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