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Molecular phylogenetic analyzes of pteridophytes based on rbcL sequence comparison

Research Project

Project/Area Number 04404003
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 植物形態・分類学
Research InstitutionThe University of Tokyo

Principal Investigator

IWATSUKI Kunio  The University of Tokyo, Faculty of Science Professor, 理学部, 教授 (10025348)

Co-Investigator(Kenkyū-buntansha) MURAKAMI Noriaki  The University of Tokyo, Faculty of Science Research Associate, 理学部, 助手 (60192770)
長谷部 光泰  東京大学, 理学部, 助手 (40237996)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥31,000,000 (Direct Cost: ¥31,000,000)
Fiscal Year 1994: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1993: ¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1992: ¥21,000,000 (Direct Cost: ¥21,000,000)
Keywordspteridophyte / rbcL / nucleotide sequence / molecular tree / Aspleniaceae / Hymenasplenium / systematics / evolution / 系統 / 分子系統 / ホウビシダ節
Research Abstract

In this study, we first developed widely-applicable method in amplifying and sequencing rbcL gene from pteridophytes. We tested many kinds of primers for PCR amplification of rbcL fragments and got universal primers for pteridophytes which is also applicable for seed plants. Performing this method with DNA sequencer which we bought by this grant, we became able to determine about 1200bp sequence of rbcL of one wild pteridophyte species in 2.5 days. We selected one species each from 32 family, 107 genera, that cover whole variation of pteridophytes, and determined their rbcL sequences. Based on them, we constructed molecular tree and global phylogenetic relationships in fern families were revealed. In this study, we also analyzed smaller groups of ferns (epecially Aspleniaceae) using the same molecular method. Aspleniaceae is one of the largest family of ferns, containing more than 700 species. Its Intrafamiliar phylogeny was poorly known. We determined rbcL sequences from 61 species of Aspleniaceae. We elucidated global phylogenetic relation in the family as well sd smaller groups in the family : genus Hymenasplenium (20 species) and H.obliquissimum species (17 samples) complex. Molecular phylogenetic analyzes based on rbcL sequencing was valid from inter-family to intra-species levels.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Hasebe et al.: "rbcL gene sequences provide evidence for the evolutionary lineages of leptosprangiate ferns." Proc.Natl.Acad.Sci.USA. 91. 5730-5734 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hasebe et al.: "A global analysis of fern phylogeny based on rbcL nucleotide sequences." Amer.Fern J.85(掲載予定). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Murakami,N.: "Systematics and Evolutionary Biology of the Fern Genus Hymenasplenium(Aspleniaceae)" J.Plant Res.108(掲載予定). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hasebe, M.et al.: "rbcL gene sequences provide evidence for the evolutionary lineages of leptosporangiate ferns." Proc.Natl.Acad.Sci.USA. Vol.91. 5730-5734 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hasebe, M.et al.: "A global analysis of fern phylogeny based on rbcL nucleotide sequences. (In press)" Amer.Fern J.Vol.85. (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Murakami, N.: "Systematics and Evolutionary Biology of the Fern Genus Hymenasplenium(Aspleniaceae) (In press)" J.Plant Res.Vol.108. (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hasebe et al.: "rbcL gene sequences provide evidene for the evolutionary lineages of leptosprangiate ferns." Proc.Natl.Acid. Sci.USA. 91巻. 5730-5734 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hasebe,M.,et al.: "Phylogenetic relationships of Ferns deduced from rbcL gene sequence." J.Mol.Evol.37(5). 476-482 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.HASEBE et al.: "Phylogenetic relatianships ferns deduced from lbcL gone Sequence" Jowrnal of Molecular Evolution. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 長谷部 光泰: "葉緑体DNAトシダ植物の系統" 遺伝. 46. 30-36 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] D.Sriboonmaetal.: "Phylogeny of Typhonium (Araceae) inferred fron Rostriction frogment analyses. of chloyoplaot DNA" Journal of Plant Research. (1993)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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