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Studies on the genes controlling lethality and development of the silkmoth, Bombyx mori.

Research Project

Project/Area Number 04404010
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 蚕糸学
Research InstitutionThe University of Tokyo

Principal Investigator

KOBAYASHI Masahiko  Professor, Department of Agr., University of Tokyo, 農学部, 教授 (60162020)

Co-Investigator(Kenkyū-buntansha) SHIMADA Toru  Research Assistant, Department of Agr., University of Tokyo, 農学部, 助手 (20202111)
NAGATA Masao  Associate Professor, Department of Agr., University of Tokyo, 農学部, 助教授 (70107407)
木下 タロウ  大阪大学, 微生物病研究所, 教授 (10153165)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥20,500,000 (Direct Cost: ¥20,500,000)
Fiscal Year 1994: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1992: ¥12,500,000 (Direct Cost: ¥12,500,000)
KeywordsLethal gene / Chromosome / Genome / Neuropeptide / RAPD / Linkage map / Mutation / Bombyx mori / 胚子発生 / 休眠 / ペプチドホルモン / ゲノム解析 / 遺伝的モザイク / 家蚕 / 前胸腺 / ホメオティック遺伝子 / PCR / エクダイソン
Research Abstract

We adopted genome mapping as a strategy to analyze lethal genes in the silkworm, Bombyx mori since we thought positional cloning would be the most direct pathway to access the lethal genes and other developent-controlling genes. 1. We constructed an integrated map of RAPDs, RFLPs and phenotypic loci on chromosome 22, by mating experiments. This map contains the sku lethal gene. 2. A development-controlling gene, the DH-PBAN gene, was mapped at -2.5cM on chromosome 22.3. The RAPD linkage map was corresponded to the conventional phenotypic map for chromosomes 7,13 and 18 as linkage groups. Some lethal genes such as Obs, are carried by these chromosomes. 4. A few RAPDs on the Z chromosome were cloned and sequenced. One of them was considered to be a single copy sequence. 5. Some RAPDs on chromosomes 7 and 13 were cloned and sequenced. 8. A major factor which control the life span and egg-laying behavior in strain xu20, was determined to be linked to a RAPD on the 29th RAPD linkage group. 6. Sequence divergence at synonymous and non-synonymous sites of the aryphorin gene was estimated from the bombycid and saturniid silkmoths. A phylogenetic tree based on the non-synonymous sites of the gene was proposed as an evolutionary relationship of silkmoths. 7. Messenger RNA for hexamerins were quantitated along develoment and between sexes in Samia cynthia ricini and Antheraea pernyi. 8. The polyhedrin genes of the B.mori nuclear polyhedrosis virus were sequenced for an amorphous polyhedron mutant and a cuboidal polyhedron mutant. We revealed several amino acid substitutions in the polyhedrin-encoding region.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Toru Shimada et al.: "Polymorphism and likage analysis of the prothoracicotropic hormone gene in the silkmoth.Bombyx mori" Genetical Research. 63(4). 190-195 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Toru Shimada et al.: "Phy logenetic relationship of silkmoths inferved from seguehce data of the arylphorin gene." Molecular Phylogenetics and Evolution. 4(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Waraporn Pinyarat et al.: "Linkase analysis of the gene encoding precuasor protein of diapause hormone and pheromone biosynthesis-activating neuropeptide in the Silkmoth,Bomloyx mori." Genetical Research. 65(2)(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Amornrat Promboon et al.: "Linkase map of randow amplificd polymovphic DNAs (RAPDS)in the silkwoum,Bombyx mori." Genetical Research. 65(3)(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Toru Shimada et al.: "Polymorphism and linkage analysis of the prothoracicotropic hormone gene in the silkmoth" Bombyx mori.Genetical Research (Cambridge). 63 (4). 190-195 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Toru Shimada et al.: "Phylogenetic relationship of silkmoths inferred from sequence data of the arylphorin gene." Molecular Phylogenetics and Evolution. Volume 4 (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Waraporn Pinyarat et al.: "Linkage analysis of the gene encoding precursor protein of diapause hormone and pheromone biosynthesis-activating neuropeptide in the silkmoth, Bombyx mori." Genetical Research (Cambridge). 65 (2) (in press).(1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Amornrat Promboon et al.: "Linkage map of random amplified polymorphic DNAs (RAPDs) in the silkworm" Bombyx mori.Genetical Research (Cambridge). 65 (3) (in press).(1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Toru Shimada et al.: "Polymorphism and linkage analysis of the prothoracicotropic hormone gene in the silkmoth,Bombyx mori." Genetical Research. 63(4). 190-195 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Toru Shimada et al.: "Phylogenetic relationship of silkmoths inferved from sequence data of the arylphorin gene." Molecular Phylogenetics and Evolution. 4(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Waraporn Pinyarat et al.: "Linkage analysis of the gene encoding precursor protein of diapause hormone and pheromone biosynthsis-activating neuropeptide in the Silkmoth,Bombyx mori." Genetical Research. 65(2)(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Amornrat Promboon et al.: "Linkage map of random amplified polymorphic DNAs (RAPDs) in the Silkworm,Bombyx mori." Genetical Research. 65(3)(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Shimada,T.Hasegawa,K.Matsumoto,N.Agui,M.Kobayashi: "Polymorphism and Linkage Analysis of the Prothoracicotropic Hormone Gene in Bombyx mori." Genetical Research(Combridge). 63(In press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Toru Shimada: "Distribution of split 5.8S ribosomal RNA in Diptera." Insect Molecular Biology. 1(1). 45-48 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 嶋田 透,小林 淳,宮田 保,永田 昌男,小林 正彦: "ヤママユが科・カイコが科のアリルフォリンの免疫拡散法による比較." 日本応用動物昆虫学会誌. 36(2). 119-126 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Toru Shimade ayd Masahiko Kobayshi: "Homeotic trousformation en ENK mutant cells is not affectul by neighvoring norwal cels in gemnetic mosaics of the selkworm,Bombyx mori" Journal of Sericultural Science of Japan. 61(5). 444-450 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 洗 幸夫,小林 正彦: "家蚕休眠卵の非休眠化におよぼすコロナ放電の影響" 日本蚕糸学雑誌. 62(1). (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 永田 昌男,土田 卓三,折笠 千登世,鈴木 昭憲: "光沢不眠蚕のエクジステロイド分泌系にいつて" 日本蚕糸学雑誌. 61(5). 400-406 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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