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Studies on te regulatory mechanisms of gene expression in early embryos

Research Project

Project/Area Number 04404016
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 畜産学(含草地学)
Research InstitutionThe University of Tokyo

Principal Investigator

TOYODA Yutaka  Univ.of Tokyo, Institute of Medical Science, Professor, 医科学研究所, 教授 (90050418)

Co-Investigator(Kenkyū-buntansha) NAITO Kunihiko  Univ.of Tokyo, Institute of Medical Science, Research associate, 医科学研究所, 助手 (20188858)
KYUWA Shigeru  Univ.of Tokyo, Institute of Medical Science, Research associate, 医科学研究所, 助手 (30177943)
IBA Hideo  Univ.of Tokyo, Institute of Medical Science, Associate professor, 医科学研究所, 助教授 (60111449)
IWAKURA Yoichirou  Univ.of Tokyo, Institute of Medical Science, Professor, 医科学研究所, 教授 (10089120)
SATO Eimei  Univ.of Tokyo, Institute of Medical Science, Associate professor, 医科学研究所, 助教授 (80093243)
浜田 博司  東京都臨床医学総合研究所, 部長 (00208589)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥18,000,000 (Direct Cost: ¥18,000,000)
Fiscal Year 1994: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1993: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1992: ¥10,000,000 (Direct Cost: ¥10,000,000)
Keywordsoocyte maturation / fertilization / cyclin / acrosin / endothelin / parthenogenesis / chimeras / adenovirus / 初期胚 / 遺伝子発現 / 性分化 / 体外受精 / 性判別 / 卵丘 / 減数分裂 / レポーター遺伝子 / 胚培養 / 顕微注入 / メタロチオネイン / ベータガラクトシダーゼ
Research Abstract

1) Gene expression during oocyte maturation
A) Maturation medium influences the fluctuation pattern of H1K activity and the biological characteristics of porcine oocytes cultured in vitro.
B) Oocyte-cumulus cell complexes influence the structure of the oviductal wall during the passage in the oviduct.
2) Gene expression during fertilization
A) Male mice homozygous for a targeted mutation in the mouse acrosin gene were still fertile.
B) Supplementing the fertilization medium with soluble factor (s) vortexed out of cumulus-oocyte complexes improved the rate of fertilization when spermatozoa were preincubated in BSA-supplemented medium.
C) Oocytes from c-mos-deficient mice matured to the second meiotic metaphase, but were activated without fertilization.
3) Gene expression during early embryogenesis
A) A steroid, progesterone, was potent to induce parthenogenetic development of unfertilized eggs.
B) ET-1^<-/-> homozygous mice die of respiratory failure at birth and have morphological abnormalities of the pharyngeal-arch-derived craniofacial tissues and organs.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (35 results)

All Other

All Publications (35 results)

  • [Publications] 豊田 裕: "Sperm from mice carrying a targeted mutatiou of the acrosin gene can penetrate the oocyte 20na pellucida and ettect tertilization" Journal of Biological Chemistry. 269. 31845-31849 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 伊庭英夫: "Analysis of AP-I tunction in cellular transformation pathways" Journal of Virology. 68. 3527-3535 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 久和 茂: "Apoptosis induced in mouse hepatitis virusinfected cells by virus-specific CD8^+ cytotoxic T-lymphocyte clone" Journal of Virology. 68. 7540-7545 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 佐藤英明: "Structural changes in the oviductal wall during passage of unfertilized cumulus-oocyte compleyes in mice" The Anatomical Record. 241. 205-210 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 岩倉洋一郎: "Augmentation of c-tos and c-jun expression in tamors in transgenic mice carrying the human T-cell leukemia virus gene" Virus Gene. (印刷中). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 内藤邦彦: "Association of p34^<cdc2> and cyclin BI during meiotic maturation in porcine oocytes" Developmental Biology. (印刷中). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 豊田 裕: "Elevated blood pressure and craniofacial abnormaliries in mice deficient in endothelin-1" Nature. 368. 703-710 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 豊田 裕: "Localization of a N-ras-related seguence on rat chromosome 1" Experimental Animals. 43. 125-127 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 佐藤英明: "Glycosaminoglycans prevent induction of hagmentation of porcine oocytu stinulated by dibutyryl cyclic adenosine 3',5'-monophosphate in culture" Cell Structure and Gunction. 19. 29-36 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 佐藤英明: "The effect of hig follicular fluid factions on cumuls expausin and male pronucleus farmation in porcine oocytes matured and fertilized in vivo" Jonrnal of Reproduchin and Fertility. 101. 667-673 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 伊庭英夫: "tra-2 promotor can respond to serum stimulation throngh AP-1 complrxes" Oncogene. 10. 689-696 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 久和 茂: "Modulatim of cellular maeromolecalar synthesis by corona virus implication for pathoglnesis" Journal of Virology. 68. 6815-6819 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Baba, T., Azuma, S., Kashiwabara, S., Toyoda, Y.: "Sperm from mice carrying a targeted mutation of the acrosin gene can penetrate the oocyte zona pellucida and effect fertilization." J.Biol.Chem.269. 31845-31849 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kurihara, Y., Kurihara, H., Suzuki, H., Komada, T., Maemura, K., Nagai, R., Oda, H., Kuwaki, T., Cao, W.H., Kamada, N., Jishage, K., Ouchi, Y., Azuma, S., Toyoda, Y., Ishikawa, T., Kumada, M., Yazaki, Y.: "Elevated blood pressure and craniofacial abnormalities in mice deficient in endothelin-1." Nature. 368. 703-710 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kunieda, T., Kobayashi, E., Tachibana, M., Imamichi, T., Toyoda, Y.: "Localization of a N-ras-related sequence on rat chromosome 1." Exp.Anim.43. 125-127 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato, E., Inoue, M., Takahashi, Y., Toyoda, Y.: "Glycosaminoglycans prevent induction of fragmentation of porcine oocytes stimulated by dibutyryl cyclic adenosine 3', 5'-monophosphate in culture." Cell Struct.Func.19. 29-36 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Daen, F.P., E.Sato, K.Naito and Y.Toyoda: "The effect of pig follicular fluid fractions on cumulus expansion and male pronucleus formation in porcine oocytes matured and fertilized in vitro." J.Reprod.Fert.101. 667-673 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato, E., N.Ando, Y.Takahashi, H.Miyamoto and Y.Toyoda: "Structural changes in the oviductal wall during the passage of unfertilized cumulus-oocyte complexes in mice." Anat.Rec.241. 205-210 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Suzuki, T., Murakami, M., Onai, N., Fukuda, E., Hashimoto, Y., Sonobe, M.H., Kameda, T., Ichinose, M., Miki, K., Iba, H.: "Analysis of AP-1 function in cellular transformation pathways." J.Virol.68. 3527-3535 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sonobe, M.H., Yoshida, T., Murakami, M., Kameda, T., Iba, H.: "fra-2 promotor can respond to serum-stimulation through AP-1 complexes." Oncogene. 10. 689-696 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kyuwa, S., Cohen, M., Nelson, G., Tahara, S.M.: "Stohlman, S.A.Modulation of cellular macromolecular synthesis by coronavirus ; implication for pathogenesis." J.Virol.68. 6815-6819 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shibata, S., Kyuwa, S., Lee, S-K., Toyoda, Y., Goto, N.: "Apoptosis induced in mouse hepatitis virus-infected cells by virus-specific CD8^+ cytotoxic T-lymphocyte clone." J.Virol.68. 7540-7545 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Naito, K., Hawkins, C., Yamashita, M., Nagahama, Y., Aoki, F., Kohmoto, K., Toyoda, Y., Moor, R.M.: "Association of p34^<odo2> and cyclin B1 during meiotic maturation in porcine oocytes." Dev.Biol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Iwakura, Y., Tosu, M., Yoshida, E., Saijo, S., Nakayama, J., Itagaki, K., Asano, M., Siomi, H., Hatanaka, M., Takeda, T., Nunoya, T., Ueda, S., Shibuya, H.: "Augmentation of c-fos and c-jun expression in tumors in transgenic mice carrying the human T-cell leukemia virus type 1 tax gene." Virus Gene. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 豊田裕: "Sperm from mice carrying a targeted mutation of the acrosin gene can penetrate the oocyte 20na pellucida and effect fertilization" Journal of Biological Chemistry. 269. 31845-31849 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 伊庭英夫: "Analysis of AP-1 function in cellular transformation pathways" Journal of Virology. 68. 3527-3535 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 久和茂: "Apoptosis induced in mouse hepatitis virus-infected cells by virus-specific CD8^+ cytotoxic T-lymphocyte clone" Journal of Virology. 68. 7540-7545 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 佐藤英明: "Structural changes in the oviductal wall during passage of unfertilized cumulus-oocyte complexes in mice" The Anatomical Record. 241. 205-210 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 岩倉洋一郎: "Augmentation of c-fos and c-jun expression in tumors in transgenic mice carrying the human T-cell leukemiq virus gene" Virus Gene. (印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 内藤邦彦: "Association of p34^<cdc2> and cyclin B1 during meiofic maturation in porcine oocytes" Developmental Biology. (印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 豊田 裕: "PCR sexing and developmental rats differences in preimplantation mouse embryos fertilited and cultured in vitro" Molecular Reproduction and Development. 35. 121-126 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 豊田 裕: "卵成熟と受精を制御する" 遺伝. 47. 22-28 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 佐藤 英明: "Accumulation of the c-fos protein in maturing oocytes in graafian follicles in mice" Journal of Reproduction and Development. 39. 243-249 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 豊田 裕: "Effcuto F priongeel sperm princabatin and elevated calaium cmcentratias on firtiligatin J cunnluo-free manoe eqqs in SEO" Trsrnal j Reprodu ction and Xevehpmenr. 38. 219-224 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 豊田 裕: "Shuin difference in the devebpmen oF preimplantation movse embryos in a medium supplerented with sugvoride dismutau or terlylevdiamire tenuaotic acid" Tnrnal F Mammaeiau Ova Pesuarch. 9. 180-190 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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