| Project/Area Number |
04404040
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | KEIO University |
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Principal Investigator |
TSUCHIYA Masaharu Internal Medicine, Keio University Professor, 医学部・内科, 教授 (60051124)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Hidekazu Internal Medicine Ass.Professor, 医学部・内科, 講師 (80186949)
MIURA Soichiro Internal Medicine Ass.Professor, 医学部・内科, 講師 (50138012)
鈴木 雅之 慶應義塾大学, 医学部, 助手 (70236008)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥21,000,000 (Direct Cost: ¥21,000,000)
Fiscal Year 1993: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1992: ¥18,000,000 (Direct Cost: ¥18,000,000)
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| Keywords | microcirculation / confocal laser microscopy / active oxygen / nitric oxide / rhodamine 123 / dichlorofluorescin / propidium iodide / ischemia-reperfusion / propidium iodide / NADH / 虚血性肝障害 / 癌細胞肝転移 / 酸素ストレス / 肝移植 / ミトコンドリア機能 |
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| Research Abstract |
We have established the experimental set up for intravital observation using confocal laser scanning imaging system. Our experiments were performed using fluorescence probes ; dichlorofluorescin diacetate (a fluorochrome sensitive to intracellular hydroperoxide formation), rhodamine 123 (a fluorochrome sensitive to the mitochondrial membrane potential, ) and propidium iodide (which labels the unclei of non viable cells).
By using above mentioned set up ;
1. It has been observed that leukocyte adhesion along postcapillary venules, the oxidative stress and the mitochondrial dysfunction occur in rat mesenteric microcirculation after ischemiareperfusion.
2. Intralobular heterogeneity of the oxidative stress and the mitochondrial dysfunction were visualized in relation to their topographic distributions with the cell death during low-flow hypoxia in perfused liver. The oxidative stress and the resultant cell death during low-fow hypoxia are spatially restricted in the intermediate zone between
… More
the periportal and pericentral regions.
3. Cold ischemia followed by reperfusion induced leukocyte adhesion to the sinusoidal wall and the damage of parenchymal cells. Superoxide dismutase attenuated these ischemiareperfusion-induced injuries.
4. The metabolic changes in tumor cells after coculturing with Kupffer cells were visualized and analyzed. In cocultured hepatoma cells and colon cancer cells, Kupffer cells induced the oxidative stress and the mitochondrial dysfunction followed by cell death. Superoxide dismutase did not inhibit the mitochondrial dysfunction and cell death. L-NMMA,an inhibitor of nitric oxide production eliminated decrease in mitochondrial energization and the tumor cell injury induced by Kupffer cells. Kupffer cell-derived nitric oxide may present tumor cell cytotoxicity via inhibiting mitochondrial dysfunction.
The metabolic changes in tumor cells were also determined ex vivo perfused liver. The oxidative stress and the mitochondrial dysfunction in tumor cells, which adhered to the liver sinusoid, were also obvious as well as those in vitro experiments. Less
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