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Fractal Growth and Morphological Change by a Cell Population

Research Project

Project/Area Number 04452053
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 物性一般(含極低温・固体物性に対する理論)
Research InstitutionCHUO UNIVERSITY

Principal Investigator

MATSUSHITA Mitsugu  Chuo University, Departmet of Physics, Professor, 理工学部, 教授 (20091746)

Co-Investigator(Kenkyū-buntansha) MATSUYAMA Tohey  Niigata University, School of Medicine, Associate Prof., 医学部, 助教授 (00047200)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 1993: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1992: ¥3,800,000 (Direct Cost: ¥3,800,000)
Keywordsbacterial colony / pattern formation / fractal / sefl-simioarity self-affinity / morphological change / 形態遷移 / 自己相似性 / 自己アフィン性 / 細胞集落 / パターン形成
Research Abstract

Bacterial colonies grow when a small amount of the cells are inoculated on the surface of nutrient-rich agar plates (substrates) and incubated appropriately. We have found that colony patterns change drastically when concentrations of nutrient and agar (controlling substrate softness) are varied. We have thus been able to establish quantitatively the morphological phase diagram of colony patterns by using Bacillus subtilis. Namely, we observed characteristic patterns such as self-similar DLA, compact but interfacially rough (self-affine) Eden-like, concentric-ring, densely branched DBM-like, and isotropic and homogeneous disk patterns. We also observed that individual bacterial cells start to able to move actively inside colonies when agar plates become softer. This active cell movement seems to trigger some morphological changes. Many rod-like bacterial species including Escherichia coli and Bacillus subtilis have been found to exhibit self-similar, DLA patterns due to the diffusion-limited condition of nutrient, when nutrient is poor and agar plates are rather hard. We can, therefore, conclude that the DLA mechanism is fairly universal for colony growth under these environmental conditions. For the isotropic and homogeneous disk colonies which are the simplest patterns we have ever observed we quantitatively measured the interfacial growth speed, population density and diffusion coefficient of individual cells inside colonies in detail. We thus elucidated that the growth of these colonies can be described by the Fisher's equation. However, we have not yet confirmed the self-affinity of Eden-like colonies, nor elucidated the growth mechanisms for other colony patterns.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] M.Ohgiwari: "Morphological Changes in Growth Phenomena of Bacterial Colony Patterns" Journal of the Physical Society of Japan. 61. 816-822 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Matsuyama: "Self-Similar Colony Morphogenesis by Gram-Negative Rods as the Experimental Model of Fractal Growth by a Cell Population" Applied and Environmental Microbiology. 58. 1227-1232 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 松下 貢: "バクテリア増殖によるパターン形成" 日本物理学会誌. 47. 441-446 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Matsuyama: "Fractal Morphogenesis by a Bacterial Cell Population" CRC Critical Reviews in Microbiology. 19. 117-135 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 松下 貢: "細菌集団によるパターン形成" 科学(岩波書店). 64. 104-113 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] J.Wakita: "Experimental Investigation on the Validity of Population Dynamics Approach to Bacterial Colony Formation" Journal of the Physical Society of Japan. 63. 1205-1211 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 松下 貢(編著): "医学・生物学におけるフラクタル" 朝倉書店, 224 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Ohgiwari: ", M.Matsushita and T.Matsuyama Morphological Changes in Growth Phenomena of Bacterial Colony Patterns" J.Phys. Soc. Jpn.61. 816-822 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Matsuyama and M.Matsushita: "Self-Similar Colony Morphogenesis by Gram-Negativ as the Experimental Model of Fractal Growth by a Cell Population" Appl. Environ. Microbiol.58. 1227-1232 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Matsuyama and M.Matsushita: "Fractal Morphogenesis by a Bacterial Cell Population" CRC Crit. Rev. Microbiol.19. 117-135 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Matsuyama, R.M.Harshey and M.Matsushita: "Self-Similar Colony Morphogenesis by Bacteria as the Experimental Model of Fractal Growth by a Cell Population" Fractals. 1. 302-311 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] J.Wakita, K.Komatsu, A.Nakahara, T.Matsuyama and M.Matsushita: "Experimental Investigation on the Validity of Population Dynmics Approach to Bacterial Colony Formation" J.Phys. Soc. Jpn.63. 1205-1211 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Matsuyama: "Fractal Morphogenesis by a Bacterial Cell Population" CRC Critical Reviews in Microbiology. 19. 117-135 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Matsuyama: "Self-Similar Colony Morphogenesis by Bacteria as the Experimental Model of Fractal Growth by a Cell Population" Fractals. 1. 302-311 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 松下 貢: "細菌集団によるパターン形成" 科学(岩波書店). 64. 104-113 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] J.Wakita: "Experimental Investigation on the Validity of Population Dynamics Approach to Bacterial Colony Formation" Journal OF the Physical Society of Japan. 63. 1205-1211 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Ohgiwari: "Morphological Changes in Growth Phenomena of Bacteriat Colony Patterns" Journal of Physical Society of Japan. 61. 816-822 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Matsuyama: "SelfーSimitar Colony Morphogenesis by GramーNegative Rods as the Experimental Model of Fractal Growth by a Cell Population" Applied and Environmental Microbiology. 58. 1227-1232 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 松下 貢: "バクテリア増殖によるパターン形成" 日本物理学会誌. 47. 441-446 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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