OPTICAL IMAGING OF AUDITORY CORTICAL ACTIVITY IN THE GUINEA PIG.
Project/Area Number |
04452209
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Research Category |
Grant-in-Aid for General Scientific Research (B)
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Allocation Type | Single-year Grants |
Research Field |
計測・制御工学
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Research Institution | TOKYO MEDICAL AND DENTAL UNIVERSITY |
Principal Investigator |
HASHIMOTO Toru TOKYO MEDICAL & DENTAL UNIV., INST.for MEDICAL & DENTAL ENGINEERING, ASSOCIATE PROFESSOR., 医用器材研究所, 助教授 (60014007)
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Project Period (FY) |
1992 – 1993
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Project Status |
Completed (Fiscal Year 1993)
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Budget Amount *help |
¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
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Keywords | OPTICAL IMAGING / AUDITORY CORTICAL ACTIVITY / VOLTAGE-SENSITIVE DYE / EPI-FLUORESCENCE / COOLED CCD VIDEO CAMERA / SUBTRACTION IMAGE / SPATIO-TEMPORAL PATTERN OF CORTICAL ACTIVITY / IMAGE SAMPLING SYNCHRONOUS WITH HEARTBEAT |
Research Abstract |
The pattern of population activity of the auditory cortex in response to sound stimulation was mapped with a newly developed video method imaging the epi-fluorescence of a voltage-sensitive dye(RH795). The animals were lightly anesthetized with Nembutal. The fluorescent images were sampled by the excitation light pulse or through a time window by an electronic shutter, which was opend in synchrony with the stimulus presentations. The background images were subtracted from the responsive images and the difference image was integrated in the video frame memory. The excitation foci in the optical record in response to a natural call of the animal were analyzed in correlation with a spectrographic survey of sound stimuli. The spatio-temporal patterns of auditory cortical activity in response to the species-specific repertoire of the animal's vocalization were found. For further analysis of excitation foci a sinusoidal click train and "terminal-analog" speech-like sounds were used as the simpler model of animal's vocalized sound. The stripe pattern of excitation found in the optical records would be due to the columnar structure in the auditory cortex. The time series of the optical records would play an important role in analyzing the global functional structure and the population activity of the auditory cortex in response to sound stimulation.
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Report
(3 results)
Research Products
(25 results)