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Role of phospholipid molecular species containing poly unsaturated fatty acids

Research Project

Project/Area Number 04453130
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 応用生物化学・栄養化学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

KITO Makoto  Research Institute for Food Science, Professor, 食糧科学研究所, 教授 (60027183)

Co-Investigator(Kenkyū-buntansha) MORIYAMA Tatsuya  Research Institute for Food Science, Instructor, 食糧科学研究所, 助手 (60239704)
URADE Reiko  Research Institute for Food Science, Instructor, 食糧科学研究所, 助手 (90167289)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥7,300,000 (Direct Cost: ¥7,300,000)
Fiscal Year 1993: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1992: ¥6,200,000 (Direct Cost: ¥6,200,000)
KeywordsArachidonic acid / Phospholipids / Collagen / Human platelets / 小胞体プロテアーゼ / ER-60プロテアーゼ / タンパク質代謝 / ホスファチジルイノシトール / ホスファチジルセリン
Research Abstract

We have indicated that several nanomolar thromboxane A_2 (TXA_2) originating from inositollipids may directly cause Ca^<2+> mobilization in human platelets during activation with collagen.
The mechanism of arachidonic acid (AA) release in collagen-activated human platelets was studied. An arachidonic acid metabolite, thromboxane B_2, was formed in parallel with the formation of phosphatidic acid without fomation of lysophosphatidic acid or lysophosphatidylinositol in the absence of extracellular Ca^<2+>, suggesting that AA was released from PI via a PI-specific phospholipase C/diacylglycerol lipase/monoacylglycerol lipase pathway under the cytosolic low Ca^<2+> concentrations. Moreover, solubilized DG lipase and MG lipase could hydrolyze the substrates at basel cytosolic free Ca^<2+> concentrations. Subsequently, the relationship of cytosolic free Ca^2 concentrations and formation of AA metabolites was analyzed using Ca^<2+> ionophore, A23187. Collagen was able to induce a release of small amounts of AA under basal cytosolic Ca^<2+> conditions. However, a release of large amounts of AA was induced by phospholipase A_2 activated by both collagen-receptor occupancy and elevated Ca^<2+> levels. A TXA_2 mimetic agonist, STA_2 induced all the responses except for AA release. From these results, the mechanism of AA release and signal transduction in collagen-activated human platelets is discussed.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] R.Urade: "Protein Degradation by the Phosphoinositide-specific Phospholipase C-α Family from Rat Liver Endoplasmic Reticulum" J.Biol.Chem.267. 15152-15159 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] R.Urade: "Inhibition by acidic phospholipids of protein degradation by ER-60 protease,a novel cysteine protease,of endoplasmic reticulum" FEBS Letters. 312. 83-86 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 森山 達哉: "イノシトールリン脂質特異的ホスホリパーゼCと情報伝達" 化学と生物. 30. 714-722 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] R.Urade: "Protein Degradation by ERp72 from Rat and Mouse Liver Endoplasmic Reticulum" J.Biol.Chem.268. 22004-22009 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 鬼頭 誠: "生体膜リン脂質の多機能性に関する生化学的研究" 日本農芸化学会誌. 67. 1047-1053 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Moriyama: "The Mechanism of Arachidonic Acid Release in Collagen-activated Human Platelets" Biosci.Biotech.Biochem.58. 93-98 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] R.Urade: "Protein Degradation by the Phosphoinositidespecific Phospholipase C-alpha Family from Rat Liver Endoplasmic Reticulum" J.Biol.Chem.267(21). 15152-15159 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] R.Urade: "Inhibition by acidic phospholipids of protein degradation by ER-60 protease, a novel cysteine protease, of endoplasmic reticulum" FEBS Letters. 312(1). 83-86 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Moriyama: "Phosphoinositide-Specific Phospholipase C" Kagaku to Seibutu. 30(11). 714-722 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] R.Urade: "Protein Degradation by ERp72 from Rat and Mouse Liver Endoplasmic Reticulum" J.Biol.Chem.268(29). 22004-22009 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Makoto Kito: "Biochemical Studies on Multifunctions of Phospholipids in Biological Membranes" Nippon Nogeikagaku Kaishi. 67(7). 1047-1053 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Moriyama: "The Mechanism of Arachidonic Acid Release in Collagen-activated Human Platelets" Biosci.Biotech.Biochem.58(1). 93-98 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Moriyama: "The Mechanism of Arachidonic Acid Release in Collagen-activated Human Platelets" Biosci.Biotech.Biochem.58. 93-98 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] R.Urade: "Protein Degradation by ERp72 from Rat and Mouse Liver Endoplasmic Reticulum" J.Biol.Chem.268. 22004-22009 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 鬼頭 誠: "生体膜リン脂質の多機能性に関する生化学的研究" 日本農芸化学会誌. 67. 1047-1053 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] R.Urade: "Protein Degradation by The Phosphoinositidespecific Phospholipase C‐α Family from Rat Liver Endoplasmic Reticulum" Journal of Biological Chemistry. 267. 15152-15159 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] R.Urade: "Inhibition by acidic phospholipids of Protein degradation by ER‐60 protease,a novel cysteine protease,of endoplasmic reticulum" FEBS Letters. 312. 83-86 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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