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Bioorganic-chemical study on Self-incompatibility of Brassica

Research Project

Project/Area Number 04453136
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 製造化学・食品
Research InstitutionThe University of Tokyo

Principal Investigator

ISOGAI Akira  Univ.of Tokyo, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (20011992)

Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 1993: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1992: ¥5,300,000 (Direct Cost: ¥5,300,000)
KeywordsSelf-incompatibility / Brassica / Brassica campestris / S-glycoprotein / S-receptor kinase / cDNA / Callose formation / Brassica Campestris / 自家不和合性, / アブラナ科植物, / プロテインキナーゼ / S糖タンパク質,
Research Abstract

1. The diversity of SLG (S-locus glycoprotein) in self-incompatibility of Brassica campestris was studied in terms of DNA sequences of SLGs and their related genes, SRAs. Three probes to screen SLG8 and SRA1 cDNA were snythesized based on the partial amino acid sequences of SLG8 and SRA1 proteins. Screening of a cDNA library obtained from the stigmas with the synthetic probes gave SLG8 and SRA1 cDNA clones. Using these two clones, a cDNA library of S12 homozygote was screened for SLG12 and SRA3 clones. Sequence analysis of these four clones and comparison of amino acids sequences deduced from these DNA sequences showed the presence of conserved regions of SLGs and the differences between SLGs and SRAs.
2. To prove the presence of SRK (S-receptor kinase) genes in B.campestris, two primers were synthesized to correspond the conserved regions in a C-terminal part of SLG and a kinase domain. PCR with synthetic primers using first strand cDNAs obtained from mRNA of a stigmas gave anticipated PCR products encoding 800Bp fragments.
3. By screening the cDNA library with the above PCR product, several clones were isolated. The cDNA sequences of SRK12 gene was deduced from the DNA sequences of these clones. It was a typical SRK gene which was composed of a SLG domain, a transmembrane domain and a kinase domain. This suggested involvement of phosphorylation in signal transduction in self-incompatibility of Brassica.
4. Study on relationship between callose formation and rejection of pollen tube growth in self pollination using some inhibitors indicated that the callose formation would be the second effect of self-pollination and not the cause of inhibition of pollen tube penetration into stigmas.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] M.Watanabe et al.: "Variation and inheritance of NS-glycoprotein in self-ncompatible Brassica campestris." Plant Cell Physiol.33. 343-351 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Yamakawa et al.: "The cDNA sequence of NS3-glycoprotein from Brassica campestris and its homology to related proteins." Plant Cell Physiol.34. 171-173 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Hinata et al.: "Recent studies on S-glycoproteins in self-incompatible with stress on Crucifers." Gamma Field Symposia. 29. 113-128 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Hinata et al.: "A review of recent studies on homomorphic self-incompatibility." Intern.Review Cytol.143. 257-296 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] I.S.Nou et al.: "Comaprison of S-alleles and S-glycoproteins between two wild populations of Brasscia campestris in Turkey and Japan." Sex.Plant Reprod.6. 79-86 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] I.S.Nou et al.: "Isolation of S-alleles from a wild population of Brassica campestrsis L.at Balcesme,Turkey and their characterization by S-glycoproteins." Sex.Plant Reprod.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Yamakawa et al.: "The sequences of S-glycoproteins involved iself-incompatibility of Brassica campestris and their distribution among Brassicaceae." Biosci.Biotech.Biochem.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 西沢直子、磯貝彰: "アブラナ科植物の自家不和合性と乳頭細胞像" 電子顕微鏡. 28. 23-29 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 渡辺正夫ら: "高等植物の自家不和合性" 生化学. 64. 1317-1329 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 渡辺正夫ら: "アブラナ科植物の自家不和合性" 組織培養. 18. 437-442 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 磯貝彰、日向康吉: "受精における自家不和合性の分子機構" 細胞. 24. 171-174 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] W.Watanabe et al.: "Variation and inheritance of S-glycoprotein in self-ncompatible Brassica campestris" Plant Cell Physiol.33(4). 343-351 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Yamakawa el al.: "The cDNA sequence of NS3-glycoprotein from Brassica campestris and its homology to related proteins" Plane Cell Physiol.34(1). 171-173 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Hinata et al.: "Recent studies on S-glycoproteins in self-incompatible with stress on Crucifers" Gamma Field Symposia. No.29. 113-129 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Hinata et al.: "A review of recent studies on homomorphic self-incompatibility" Intern.Review Cytol.143. 257-296 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] I.S.Nou et al.: "Comparison of S-alleles and S-glycoproteins between two wild populations of Brasscia campestris in Turkey and Japan" Sex.Plant Reprod.6. 79-86 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] I.S.Nou et al.: "Isolation of S-alleles from a wild population of Brassica campestrsis L.at Balcesme, Turkey and their characterization by S-glycoproteins" Sex.Plant Reprod.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Yamakawa et al.: "The sequences of S-glycoproteins involved itself-incompatibility of Brassica campestris and their distribution among Brassicaceae" Biosci.Biotech.Biochem.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] N.Nishizawa and A.Isogai: "Ultrastructure of papillar cells revealed by liquid helium rapidfreezing and substitution-fixation method and self-incomatibility in cruciferous plants" Dennshi-kennbikyou. 28(1). 23-29 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Watanabe et al.: "Self-incompatibility in higher plants" Seikagaku. 64(11). 1317-1329 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Watanabe et al.: "Self-incompatibility in Brassicaceae" Saiboubaiyou. 18(13). 437-442 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] A.Isogai and K.Hinata: "Molecular mechanism of Self-incompatibility in flowering plants" Saibou. 24(4). 171-174 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] I.S.Nou et al.: "Isolation of S-alleles from a wild population of Brassica campestrsis L.at Balcesme,Turkey and their characterization by S-glycoproteins." Sex.Plant Reprod.(in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] S.Yamakawa et al.: "The sequences of S-glycoproteins involved iself-incompatibility of Brassica campestris and their distribution among Brassicaceae." Biosci.Biotech.Biochem.(in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Watanabe: "Variation and inheritance of NS-glycoprotein in self-incompatible Brassica campestris." Plant Cell Physiol.33. 343-351 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] S.Yamakawa: "The cDNA sequence of NS3-glycoprotein from Brassica campestris and its homology to related proteins." Plant Cell Physiol.,. 34. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] I.S.Nou: "Isolation of S-alleles from a wild population of Brassica campestrsis L.at Balcesme,Turkey and their characterization by S-glycoproteins." Sex.Plant Reprod.6. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 磯貝 彰: "受精における自家不和合性の分子機構." 細胞. 24. 171-174 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Hinata: "Recent advances of homomorphic self-incompatibility studies," Int.Rev.Cytol.143. 257-296 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 渡辺 正夫: "高等植物の自家不和合性." 生化学,. 64. 1317-1329 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 磯貝 彰: "「開花・結実の分子機構」" 秀潤社,27 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 磯貝 彰: "「植物分子・細胞工学マニュアル」" 講談社サイエンティフィック,8 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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