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ANALYSIS OF STRESS RESPONCES IN SOME MEDICINAL PLANT CELL CULTURES

Research Project

Project/Area Number 04453149
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Chemical pharmacy
Research InstitutionTHE UNIVERSITY OF TOKYO

Principal Investigator

EBIZUKA Yutaka  THE UNIVERSITY OF TOKYO, FACULTY OF PHARMACEUTICAL SCIENCES, ASSOCIATE PROFESSOR, 薬学部, 助教授 (90107384)

Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1993: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1992: ¥4,700,000 (Direct Cost: ¥4,700,000)
KeywordsMedicinal plants / Cell cultures / Stress / Elicitor / Pueraria lobata / Ephedra distachya / Isoflavonoid / Lignin / イソフラボン配糖体 / アシルアミノ酸 / ファイトアレキシン / イーストエキス / マンナン糖タンパク
Research Abstract

1. Among the enzymes involved in isoflavonoid biosynthesis in Pueraria lobata, complementary as well as genomic DNAs encoding chalcone synthase (CHS) and reductase (CHR) co-acting with CHS have been cloned and sequenced. In the promoter regions of both genomic clones, elicitor responding elements reported in Phaseolus vulgaris were identified. Plasmid containing this 500 bp region connected to beta-glucuronidase (GUS) gene as a reporter was constructed. Hairy roots of tobacco plant transformed with this plasmid expressed GUS protein responding to the elicitation stress. This type of promoter is useful for the time and tissue specific expressions of foreign genes in plants.
2. Elicitor inducible isoflavonoid metabolism in Pueraria lobata consisted of two distinct phases. In the first phase (0-4 hr after elicitation), constitutive isoflavone malonylglucosides (IMG) were bound to cell walls by lignification. Reaccumulation of IMG in the second phase (6-24 hr after elicitation) was brought about by de novo biosynthesis. The rapid lignification of isoflavonoid found in the first phase as a consequence of disruption of subcellular compartmentation of IMG and hydrolytic enzymes. This type of responce has never been reported in Legume plants, however, it can possibly function as a rapid defense mechanism prior to others which require de novo biosynthesis of enzymes.
3. Elicitor active components of yeast extract which induce p-coumarolyamino acids in Ephedra distachya cultures were separated, purified and identified as mannanglycoprotein. This is the first demonstration of active elicitor having mannan structure as the main sugar component D-Alanine was also found to be an elicitor active component of yeast extract. A new D-aminoacid specific p-coumaroyltransferase was detected in E.distachya cultures which serves as a key enzyme in the biosynthesis of p-coumarolyamino acids.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] H.-H.Park: "Elicitor-inducible metabolism of isoflavonoids in Pueraria lobata cell suspension cultures." Phytochemistry. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.-H.Park: "Metabolic fate of isoflavonoids and oxidative burst in elicited Pueraria lobata cells." Phytochemistry. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.-S.Song: "Mannnan glycopeptide elicits p-coumaroylamino acids in Ephedra distachya cultures." Phytochemistry. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.-S.Song: "D-Alanine elicits p-coumaroylamino acids in Ephedra distachya cultures." Phytochemistry. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.-H.Park, T.Hakamatsuka, U.Sankawa and Y.Ebizuka: "Elicitor-inducible metabolism of isoflavonoids in Pueraria lobata cell suspension cultures" Phytochemistry. (submitted). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.-H.Park, T.Hakamatsuka, U.Sankawa and Y.Ebizuka: "Metabolic fate of isoflavonoids and oxidative burst in elicited Pueraria lobata cells" Phytochemistry. (submitted). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.-S.Song, U.Sankawa and Y.Ebizuka: "Mannan glycopeptide elicits p-coumaroylamino acids in Ephedra distachya cultures" Phytochemistry. (submitted). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.-S.Song, U.Sankawa and Y.Ebizuka: "D-Alanine elicits p-coumaroylamino acids in Ephedra distachya cultures" Phytochemistry. (submitted). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.-H.Park: "Elicitor-inducible metabolism of isoflavonoids in Pueraria lobata cell suspension cultures." Phytochemistry. (発表予定).

    • Related Report
      1993 Annual Research Report
  • [Publications] H.-H.Park: "Metabolic fate of isoflavonoids and oxidative burst in elicited Pueraria lobata cells." Phytochemistry. (発表予定).

    • Related Report
      1993 Annual Research Report
  • [Publications] K.-S.Song: "Mannnan glycopeptide elicits p-coumaroylamino acids in Ephedra distachya cultures." Phytochemistry. (発表予定).

    • Related Report
      1993 Annual Research Report
  • [Publications] K.-S.Song: "D-Alanine elicits p-coumaroylamino acids in Ephedra distachya cultures." Phytochemistry. (発表予定).

    • Related Report
      1993 Annual Research Report
  • [Publications] Kyung-Sik Song: "N-Acylamino acids from Ephedra distachya cultures." Phytochemistry. 31. 823-826 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Takashi Hakamatsuka: "Isoflavone dimers from yeast extract-treated cell suspension cultures of Pueraria lobata." Z.Naturforsch.47C. 177-182 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hyung-Hwan Park: "Isoflavone glucosides exist as their 6"-0-malonyl esters in Pueraria lobata and its cell suspension cultures." Chem.Pharm.Bull.40. 1978-1980 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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