| Project/Area Number |
04454002
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
遺伝学
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| Research Institution | NARA INSTITUTE OF SCIENCE AND TECHNOLOGY (1994)
Osaka University (1993)
Nagoya University (1992) |
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Principal Investigator |
TAKAHASHI Naoki NARA INSTITUTE OF SCIENCE AND TECHNOLOGY,GRADUATE SCHOOL OF BIOSCIENCE,PROFESSOR, バイオサイエンス研究科, 教授 (30179501)
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1994: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1993: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1992: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | GENE / NERVOUS SYSTEM / DEVELOPMENT / TRANSCRIPTIONAL REGULATION / PCR / MOLECULAR BIOLOGY |
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| Research Abstract |
The homeobox is a 183-base-pare DNA sequence originally found in Drosophila segmentation and homeotic genes. InDrosophila, homeotic genes are clustered in the Antennapedia and Bithorax complexes, collectively called the homeotic gene coplex (HOM-C). In the mouse genome, about 40 homeobox genes (Hox) are clustered in four chromosomal regions (Hox A to D). The Hox genes are arranged in the same order and have the same antero-posterior patten of expression as thier structural homologue in the HOM-C,suggesting that they control mouse pattern formation in the same way that HOM-C members do in Drosophila. Homeobox gene products are believed to be transcription factors that regulate expression of target genes. A few candidate target genes have been identified in Drosophila by various approaches but the Hox gene targets are poorly understood, mostly because of limitations in the available approaches. In this research project we identify several candidate Hox gene targets, including a mouse homologue if the Drosophila tumor-suppressor gene l (2) gl, by immunopurification of DNA sequences bound to a Hox protein in native chromatin.
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