• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Cell differentiation and pattern formation in a cell-cycle dependent manner in slime mold cells

Research Project

Project/Area Number 04454018
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 植物形態・分類学
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

MAEDA Yasuo  東北大学, 理学部, 教授 (50025417)

Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 1993: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1992: ¥3,400,000 (Direct Cost: ¥3,400,000)
Keywordscellular slime mold / cell cycle / cellular differentiation / cell sorting / pattern formation / PS-point / Dictyostelium discoideum / 増殖 / 分化 / 集合中心 / リン酸化タンパク質
Research Abstract

The sorting behaver of Dictyostelium discoideum Ax-2 cells and its relation to the cell-cycle phase at the onset of starvation were analyzed with reference to pattern formation, using beta-galactosidase as a cell maker and the temperature shift method for cell synchronization. Cells (T7) starved just before the PS-point from which cells enter the differentiation phase when starved aggregated most rapidly and possibly functioned as aggregation centers, but were eventurally sorted out to the posterior zone of migrating slugs. In contrast, T1 cells starved just after the PS-point exhibited slower aggregation compared with T7 cells. During further culture, T1 cells then sorted out to the apical tips of tipped aggregates and were located predominantly in the anterior zone of migrating slugs.
The gene expressions associated with the swotchover from cell proliferation to differentiation were also analyzed using the differential plaque hybridization. As a result, Quit1 was cloned as a specifically expressed gene when cells were starved just beforethe PS-point. The coding region of Quit1 gene was identical with that of the cAMP recepter 1 gene, which is essential for development. The Quit1 mRNA was asecifically expressed just after starvation of cells around the PS-point, thus indicating the importance of the cAMP recepter 1 for cellular differentiation as well as the actual existence of the PS-point.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Akiyama,M.: "Possible involvements of 101kDa,90kDa,and 32kDa phosphoproteins in the phase-shift from growth to differentiation" Differentiation. 51. 79-90 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Maeda,Y.: "Cross-talks required for the acquisition of development competence in Dictyostelium discoideum" Animal Biology. 1. 145-155 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Maeda,Y.: "Pattern formation in a cell-cycle dependent manner during the development of Dictyostelium discoideum" Develop.Growth & Differ.35. 609-616 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Furukawa,T.: "K252a,a potent inhibitor of protein kinases promotes the transition of Dictyostelium cells from growth to differentiation" Zoological Science. 11. 69-76 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Araki,T.: "Cell-cycle dependent sorting in the development of Dictyostelium cells" Developmental Biology. 162(印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Abe,F.: "Precise expression of the cyclic AMP receptor 1(CAR1)gene during transition from growth to differentiation in Dictyostelium discoideum" FEBS Letters. (印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Akiyama, M.: "Possible involvements of 101kDa, 90kDa, and 32kDa phosphoproteins in the phase shift of Dictyostelium cells from growth to differentiation" Differentiation. 51. 79-90 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Amagai, A.: "The ethylene action in the development of cellular slime molds : An analogy to higher plants" Protoplasma. 167. 159-168 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Suzuki, T.: "Cyclic AMP and Ca^<2+> as regulators of zygote formation in the cellular slime mold Dictyostelium mucoroides" Differentiation. 167. 127-132 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Maeda, Y.: "Cross-talks required for the acquisition of development competence in Dictyostelium discoideum" Animal Biol.1. 145-155 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kawai, S.: "Promotion of zygote formation by protein kinase inhibitors during the sexual development of Dictyostelium mucoroides" Develop. Growth & Differ.35. 601-607 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Maeda, Y.: "Pattern formation in a cell-cycle dependent manner during the development of Dictyostelium dicoideum" Develop. Growth & Differ.35. 609-616 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Furukawa, T.: "K252a, a potent inhibitor of protein kinases, promotes the transition of Dictyostelium cells from growth to differentiation" Zool. Sci.11. 69-76 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Araki, T.: "Cell-cycle dependent sorting in the development of Dictyostelium cells" Develop. Biol.162, (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takeuchi, I.: "Cell differentiation and pattern formation" Int. J.Develop. Biol.(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Abe, F.: "Precise expression of the cyclic AMP receptor 1 (CAR1) gene during transition from growth to differentiation in Dictyostelium dicoideum" FEBS Lett.(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Akiyama: "Possible involvements of 101KDa,90KDa,and 32KDa phosphoproteins in the phase-shift of Dictyostelium cells from growthto differentiatior" Differentiation. 51. 79-90 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Maeda: "Cross-talks required for the acquisition of development competence in Dictyostelium discoideum" Animal Biolgy. (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 前田 靖男: "粘菌の形態形成因子" 細胞. 24. 8-13 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Suzuki: "Cyclic AMP and Ca_<2+> as regulators of zygote formation in the cellular slime mold Dictyostelium mucoroides" Differentiation. 49. 127-132 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] A,Amagai: "The ethylene action in the development of cellular slime molds: An analogy to higher plants" Protoplasma. 167. 159-168 (1992)

    • Related Report
      1992 Annual Research Report

URL: 

Published: 1992-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi