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Elastic structure in giant myofibrillar sarcomeres

Research Project

Project/Area Number 04454023
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionChiba University

Principal Investigator

MARUYAMA Kohsaku  Dept.Biology, Fac.Sci., Chiba Univ., Professor, 理学部, 教授 (60012267)

Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1993: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1992: ¥4,800,000 (Direct Cost: ¥4,800,000)
KeywordsElastic protein / Connectin / Titin / Giant sarcomeres / Crayfish / Claw muscle / 筋弾性蛋白質 / ミニタイチン
Research Abstract

In arthropod muscles, it has been known that projectin, 1200 kDa elastic protein (260 nm long), links the myosin filamentto the Z line in a sarcomere. In vertebrate skeletal muscle, connectin (titin), 3000 kDa elastic protein, does so instead.
How is the elastic structure in the giant sarcomeres of arthropod claw muscle? The sarcomere length of crayfish claw muscle is as long as 10 mum at rest. Projectin can not cover several mum distance.
In the present work it was observed that 3000 kDa connectin like protein links the Z line to the myosin filamentin crayfish closer and opener muscles. Antibodies to the 3000 kDa protein bound to the very thin filaments linking the Z line to the edge of the myosin filament after actin filaments were removed by the gelsolin treatment. Projectin was located to the surface of the myosin filament.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 丸山工作他: "Connectin,giant elastic protein,in giant sarcomeres of Crayfish claw muscle" J.Muscle Res.Cell Motil.14. 654-665 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 丸山工作他: "Elastic filaments in cardiac muscle" J.Cell Biol.120. 711-724 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 丸山工作他: "The role of a thiol protease in proteolysis of connectin in rabbit skeletal muscle myofibrils" Biomed.Res.14. Sup.2. 89-92 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 丸山工作他: "A novel domain sequence of connectin localized at the I band of skeletal muscle sarcomeres" Biochem.Biophys.Res.Comm.194. 1288-1291 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 丸山工作他: "Infrared dichroism of an elastic portion of connectin" Proc.Jpn.Acad.68B. 224-226 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 丸山工作他: "Characterization of β-connectin by dynamic light scattering" Biophys.J.65. 1906-1915 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 丸山工作 他: "Connectin,giant elastic protein,in giant sarcomeres of crayfish claw muscle" J.Muscle Res.Cell Motil.14. 654-665 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] S.Kimura,T.Matsuura,S.Ohtsuka,K.Maruyama: "Characterization and localization of α-counectin on elastic protein from rabbit skaletal musle" J.Muscle Res.Cell Motil.13. 39-47 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Higuchi,T.Suzuki,S.Kimura,K.Maruyama: "Localization and elasticity of connectin filaweats in bwg skinned mus le fibers." J.Muscle Res.Cell Motil.13. 285-294 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Ohmura,Y.Nakauchi,S.Kimura,K.Maruyama: "Auto phospborylation of β-connectin in Vitoo" Biochesn,Biophys,Res,Comm,. 183. 31-35 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] S.Ohtsuka,S.Kimura,Y.Kawamura,K.Maruyama: "Chiclcen leg muscle α-connectin as studud by a monoclonal autibody to 1200 leDa fragmout" Comp.Biochem.Physiol.103B. 543-546 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Funatsu,E.Kono,H.Higuchi,S.Kimura: "Elastic filaments in casdiac muscle" J.Ccll.Biol.120. 711-724 (1993)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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