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Molecular Mechanisms underlying Allo-recoguition in Ascidians

Research Project

Project/Area Number 04454024
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionTokyo Institute of Technology

Principal Investigator

HOSHI Motonori  Tokyo Institute of Technology, Dept. of Life Science ・ professor, 生命理工学部, 教授 (20012411)

Co-Investigator(Kenkyū-buntansha) CHIBA Kazuyoshi  Tokyo Institute of Technology Dept. of Life Science ・ Assistant Professor, 生命理工学部, 助手 (70222130)
MATSUMOTO Midori  Tokyo Institute of Technology Dept. of Life Science ・ Assistant Professor, 生命理工学部, 助手 (00211574)
TANIGUCHI Masaru  Chiba Univ. Fac. of Medicine ・ Professor, 医学部, 教授 (80110310)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 1993: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1992: ¥3,600,000 (Direct Cost: ¥3,600,000)
KeywordsAllo-recognition / Ascidians / Self-sterility / Contant Reaction / Hemocyte / Sperm Binding / Chimeric Embryos / Pheno Oxidase / 組織適合抗原 / 受精
Research Abstract

The contact reaction of hemocytes and self-sterility of gametes are typical examples of all-recognition in the ascidians We have found in Halocymhia roreizi that the contact reaction is accompanied with a burst of oxygen consumption by phenol oxidase. Basing upon this finding, we developed a method to quantify the contact reaction Similar bursts were observed when hemocytes were mixed eggs or follicle cells from different individuals.
Sperm binding to the vitelline coat is a step of allo- recognition in Ciona imtesinalis. Although H.roreizi is strictly self-stcrile, autologous as well as homologous spem bound to the vitelline coat. However, autologous sperm did not block fertilozation by homologous sperm at all, suggesting that binding of autologous sperm is phusiologically meaningless for fertilization. A highly hydrophobic glycoprotein of 70 kDa isolated from H.roretzi vitcllinc coats was purificd as a sperm-receptor. Thc N-terminal eicosapeptide and four other fragments (sixty residues in total)of this protein were sequenced.
In order to answer a question whether self-sterility is purely genetic or it depends to some extent on a kind of clonal selection during devclopment, 8-ccll embryos of C.intestinalis were dissected into left and right halves to make chimeric chimeric embryos. No sexually matured chimeras were so for obtained.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] R.De Santis: "Evidence that Metalloendoproteases Are Involved in Gamete Fusion of Ciona intestinalis,Ascidia" Develop.Biol.153. 165-171 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] R.Marino: "Purification and Characterization of A Vitelline Coat Lysin from Ciona intestinalis Spermatozoa" Mol.Reprod.Develop.32. 383-388 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Takizawa: "Effects of Protease Inhibitors on Binding of Sperm to the Vitelline Coat of the Ascidian Eggs:Implications for Participation of Proteasome(Multicatalytic Proteinase Complex)" J.Exp.Zool. 267. 86-91 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Hoshi: "Glycosidases,Proteases and Ascidian Fertilization"

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] A.Ushiyama: "Specific Binding of Acrosome Reaction-Inducing Substance to the Head of Starfish Spermatozoa" Zygote. 1. 121-127 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Chiba: "Induction of Starfish Oocyte Maturation by the βγ-subunit of Starfish G Protein and Possible Existence of the Subsequent Effector in Cytoplasm" Mol.Biol.Cell. 4. 1027-1034 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Hoshi: "Glycosphingolipids:Important Membrane Components Rather Neglected in Biotechnology" Biol.Internat.special issue, 112 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] R.De Santis.: "Evidence that Metalloendoproteases Are Involved in Gamete Fusion of Ciona intestinalis, Ascidia" Develop. Biol.153. 165-171 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] R.Marino.: "Purification and Characterization of A Vitelline Coat Lysin from Ciona intestinalis Spermatozoa" Mol. Reprod. Develop.32. 383-388 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Takizawa: "Effects of Protease Inhibitors on Binding of Sperm to the Vitelline Coat of the Ascidian Eggs. Implications for Participation of Proteasome (Multicatalytic Proteinase Complex)" J.Exp. Zool.267. 86-91 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Hoshi: "Glycosidases, Proteases and Ascidian Fertilization" Seminars Dev. Biol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] A.Ushiyama: "Specific Binding of Acrosome Reaction-Inducing Substance to the Head of Starfish Spermatozoa" Zygote. 1. 121-127 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Chiba: "Induction of Starfish Oocyte Maturation by the Br-subunit of Starfish G Protein and possible Existence of Subsequent Effector in Cytoplasm" Mol. Biol. Cell. 4. 1027-1034 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Hoshi: "Glycosphingolipids Important Membrane Components Rather Neglected in Biotechnology" Biol. Internat. special issue. 112. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Motonori Hoshi: "Effects of Protease Inhibitors on Sperm Binding to the Vitelline Coat of the Ascidian Eggs:Implication for Participation of Proteasome(Multicatalytic Proteinase Complex)" J.Exp.Zool.(in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] Motonori Hoshi: "Purification and Characterization of A Vitelline Coat Lysin from Ciona intestinalis Spermatozoa" Mol.Reprod.Develop.32. 383-388 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Motonori Hoshi: "Metalloendoproteases Are Involved in Gamete Fusion of Ciona intestinalis" Develop.Biol.153. 165-171 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Masaru Taniguchi: "Extrathymic differentiation of a T cell bearing Invariant Vα14Jα281 TCR" Intern.Rev.Immunol.(in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] Midori Matsumoto: "Studies on the vitelline coat 70kDa glycoprotein in the Ascidian,Halocynthia roretzi." The J.Reproduction and Development. 39. 108 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Motonori Hoshi: "Specific Binding of Acrosome Reaction-Inducing Substance to the Sperm Head in Starfish" Zygote. 1. 121-127 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 星 元紀(分担): "細胞社会のグリオバイオロジー" 講談社サイエンチフィク, 238 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 星 元紀(分担): "配偶子の糖鎖「今なぜ糖鎖か」第1巻" 東京化学同人(印刷中),

    • Related Report
      1993 Annual Research Report
  • [Publications] Marino,R.,De Santis,R.Hirohashi,N.,Hoshi,M.,Pinto M.R.and Usui N.: "Purification and Characterization of A Vitelline Coat Lysin from Ciona intestinalis Spermatozoa." Mol.Reprod.Develop.32. 383-388 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] De Santis,R.,Miyazaki,S.,Shirakawa,H.,Nakada,K.,Hoshi,M.,Marino,R.and Pinto M.R.: "Metalloendoproteases Are Involved in Gamete Fusion of Ciona intestinalis." DeV.Biol.153. 165-171 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Amano,T.,Okita,Y.and Hoshi,M.: "Treatment of Starfish Sperm with Egg Jelly Induces the Degradation of Histones." Develop.Growth,Differ.34. 99-106 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kubo,H.,Jiang,G.,Irie,A.,Morita,M.,Inagaki,F.,Matsubara,T.and Hoshi,M.: "A Novel Ceramide Trihexoside from the Eggs of the Sea Urchin,Hemicentrotus pulcherrimus." J.Biochem.111. 726-731 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Chiba,K.,Tadenuma,H.,Matsumoto,M.,Takahashi,K.,Katada,T.and Hoshi,M.: "The Primary structure of the α-subunit of a starfish G protein involved in 1-methyladenine-induced oocyte maturation" Eur.J.Biochem.,. 207. 833-838 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 松本 緑: "DNA解析による種内遺伝的多型の研究法" 比較生理生化学. 9. 141-147 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hoshi,M.,Okinaga,T.,Kontani,K.,Araki,T.and Chiba,K.: "Acrosome Reaction-Inducing Glycoconjugates in the Jelly Coat of Starfish Eggs.In “Comparative Spermatology,20Years After"(Serono Symposia Publication Series,Vol.75)" ed.B.Baccetti,Raven Press,NewYork,1106 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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