• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Specification of the dorsoventral axis in amphibian embryos

Research Project

Project/Area Number 04454027
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

YAMANA Kiyotaka  Kyushu Univ., Facul. Sci., Prof., 理学部, 教授 (20037162)

Co-Investigator(Kenkyū-buntansha) YUGE Masahiro  Fukuoka Women's Univ., Facul. Domes. Sci. Lecturer, 家政学部, 講師 (80220523)
NOMURA Kazuya  Kyushu Univ., Facul. Sci., Assoc. Prof., 理学部, 助教授 (30150395)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1992: ¥4,800,000 (Direct Cost: ¥4,800,000)
KeywordsDorsoventral axis / Body axis / Xenopus laevis / Early development / Determinant
Research Abstract

Prior to the beginning of development, the dorsoventral axis is specified in a newly laid egg of Xenopus laevis. The specification of this axis enables transformation of radial symmetry into bilateral symmetry, an event which is a prerequisite for subsequent body formation. Recently, we have shown that the cytoplasm taken from dorsal cells of an early embryo has also the activity to induce the dorsoventral axis when injected into ventral cells of a recipient. The purpoase of the present investigation is to identify the molecule responsible for the axis-inducing activity and to analyze the mechanism by which the axis is specified.
First, we homogenized dorsal cells of early embryos and obtained a soluble fraction, and showed that the fraction retained the axis-inducing activity. Then, to purify an active cytoplasmic material, we fractionated a soluble fraction of eggs and early embryos into several fractions according to the molecular size of proteins contained. It has been shown that Fr … More action 3 is the most active in inducing the dorsoventral axis. Proteinase K treatment, but not RNase A treatment, lowered the activity below a 50% level.
Second, we studied the timing of appearance and the distribution of the axis-inducing activity by assaying a number of cytoplasmic samples of different regions of eggs and embryos at different stages. The obtained results have revealed that the activity is present around the vegetal pole in an egg before cortial rotation. After rotation, the activity is now located at the future dorsal equatorial region of an embrho, and the movement of activity to the dorsal region depends on cortical rotation. When cortical rotation is stopped by UV irradiation the activity remains around the vegetal pole. Such irradiated eggs later developed into embryos that lacked the dorsoventral axis. Furthermore, cutting an irradiated embryo along the first cleavage plane rescures the axis probably by causing the movement of activity during wound healing. It is therefore concluded that cortical rotation is in some way involved in the movement of activity to the equatorial region and is in some way involved in the movement of activity to the equatorial region and this movement is indispensable for the specication of the dorsoventral axis. Less

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] M.Fujisue: "Occurrence of dorsal axis-inducing activity around the vegetal pole of an uncleaved egg and displacement to the subequatorial region by cortical rotation" Development. 118. 163-170 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 山名清隆: "動物の背腹は卵の時代にきまる" 「卵から親へ 生命体の発生」第6回「大学と科学」公開シンポジウム組織委員会編. 54-63 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 弓削昌弘: "アフリカツメガエルの背腹軸形成" Annual Review細胞生物学. 12. 213-221 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 弓削昌弘: "両生類における背腹軸の決定機構とその因子" 細胞工学. 12. 415-421 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 山名清隆: "カエルの体づくり" 共立出版株式会社. 144 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Fujisue, Y.Kobayakawa and K.Yamana: "Occurrence of dorsal axis-inducing activity around the vegetal pole of an uncleaved egg and displacement to the subequatorial region by cortical rotation" Development. 118. 163-170 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Yamada: "The dorsoventral axis is specified in an egg (in Japanese)" From Egg to Adult - Development. 54-63 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Yuge and K.Yamana: "Formation of the dorsoventral axis in Xenopus laevis (in Japanese)" Annual Review of Cell Biology. 213-221 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Yuge and K.Yamana: "Determination of the dorsoventral axis in Amphibians (in Japanese)" SAIBO KOGAKU (Cell Engineering). 12. 415-421 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Yamana: Body Formation of a Frog (in Japanese). Kyoritsu Shuppan Co., pp.144 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Fujisue: "Occurrence of dorsal axis-inducing activity around the vegetal pole of an uncleaved egg and displacement to the subequatorial region by cortical rotation" Development. 118. 163-170 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 山名清隆: "カエルの体づくり" 共立出版株式会社. 144 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 弓削昌弘: "両生類における背腹軸の決定機構とその因子" 細胞工学. 12. 415-421 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Fujisue: "Occurrence of dorsal axis-inducing activity around the vegetal pole of an uncleaved egg and displacement to the subequatorial region by cortical rotation." Development. (1993)

    • Related Report
      1992 Annual Research Report

URL: 

Published: 1992-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi