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Mutational and tissue culture analysis of regulatory mechanism in rice embryogenesis

Research Project

Project/Area Number 04454039
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Breeding science
Research InstitutionUniversity of Tokyo

Principal Investigator

NAGATO Yasuo  University of Tokyo, Fac., Agr.Associate Professor, 農学部, 助教授 (10143413)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Kaoru  University of Tokyo, Fac., Agr.Assistant Professor, 農学部, 助手 (70183994)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1993: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1992: ¥4,400,000 (Direct Cost: ¥4,400,000)
KeywordsRice / Embryogenesis / Mutation / Adventitious embryo / Regulatory mechanism / Tissue culture / 器官分化 / シュート / 根
Research Abstract

To date, we have identified 150 single, recessive mutations affecting embryogenesis in rice. Phenotypic characterization enabled them to be categorized into seven groups. 1. lethality at the early globular stage, 2. deletion of basal region, 3. deletion of ogans, 4. modified embryo size, 5. abnormal organ position, 6. abnormal mprphology of organs, and 7. variable abnormal phenotypes in spite of single nutation. This categorization indicates at least five regulatory processes are operating during embryogenesis : 1. pattern formation, 2. determination of organ differentiation, 3. regulation of embryo size, 4. regulation of organ position, and 5. morphogenesis of organs. In each process, two types of genes are detected ; one functioning in the whole embryo and the other functioning locally, in a specific organ. The present study also revealed that embryo size was controlled in two ways. When endosperm is reduced by the mutation of endosperm-specific gene, scutellum becomes giant without affecting shoot and root sizes. On the other hand, embryo-specific genes thus far identified regulate every organ size.
We have isolated a clone which was expressed specifically in the callus cultured for seven days on a embryo-inducing medium. This clone is expressed in zygotic embryo but not in shoots, roots or flowers. another clone is expressed commonly in embryo-inducing medium and adventitious bud-inducing medium, but not callus-inducing medium. These two clones would play important roles in embryogenesis and organ differentiation.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Tamura,Y.: "A gene profoundly affecting shoot organization in the early phase of rice development." Plant Science. 82. 91-99 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 高原美規: "イネの初期幼胚の培養" 組織培養. 18. 314-318 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kitano,H.: "Hierarchical regulation of organ differentiation during embryogenesis in rice." The Plant Journal. 3. 607-610 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 洪淳寛: "胚形成過程の遺伝学的解析" 組織培養. 20(印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tamura, Y.: "A gene profoundly affecting shoot organization in the early phase of rice development" Plant Science. 82. 91-99 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takahara, Y.: "Culture of early young embryo in rice" Soshiki-Baiyou. 18. 314-318 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kitano, H.: "Hierarchical regulation of organ differentiation during embryogenesis in rice" The Plant Journal. 3. 607-610 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hong, S.K.: "Genetic analysis of embryogenesis" Soshiki-Baiyou. 20(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kitano,H.: "Hierarchical regulation of organ differentiation during embryogenesis in rice." The Plant Journal. 3. 607-610 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 洪 淳寛: "胚形成過程の遺伝学的解析" 組織培養. 20(印刷中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.Tamura: "A gene profoundly affecting shoot organization in the early phase of rice development" Plant Science. 82. 91-99 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Kitano: "Hierarchical regulation of organ differentiation during embryogenesis in rice" The Plant Journal. 3. (1993)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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