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Study on glutelin genes of storage proteins in rice.

Research Project

Project/Area Number 04454041
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Breeding science
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

SATOH Hikaru  KYUSHU UNIV., FAC.AGR., ASSOCIATE PROF., 農学部, 助教授 (70128031)

Co-Investigator(Kenkyū-buntansha) OGAWA Masahiro  YAMAGUCHI WOMEN'S UNIV., FAC.HOME ECONOMICS,ASSOCIATE PROF., 家政学部, 助教授 (10160772)
YOSHIMURA Atsushi  KYUSHU UNIV., FAC.AGR., ASSOCIATE PROF., 農学部, 助教授 (00182816)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1994: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1993: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1992: ¥3,200,000 (Direct Cost: ¥3,200,000)
KeywordsRice / Improvement of grain properties / Genetic resources / Storage protein / Glutelin / Gene analysis / SDS-PAGE / IEF / グリテリン / SOS-PAGE / 突然変異体
Research Abstract

Two major subunits of rice glutelin, the acidic (alpha) and basic (beta) subunits consist of various polypeptides in their apparent molecular mass and pI.We found a wide variation among the rice atrains in the apparent molecular mass and pI by SDSPAGE and IEF,especially between Japonica and Indica types. The glutelin of "Kinmaze", Japonica type, and "IR36", Indica type, were analyzed exactly. In SDSPAGE analysis, the glutelin was separated into four alpha subnit bands, alpha-1, alpha-2, alpha-3 and alpha-4, and four beta subunit bands, beta-1, beta-2, beta-3 and beta-4.The alpha-3 of "Kinmaze" moved faster than that of "IR36". In IEF analysis, alpha and beta subunits had at least 12 and 9 bands, respectively. Several polymorphic hands were detected in both subunits. The relation between the apparent molecular mass and pI of the polypeptides were analyzed by 2-dimensional electrophoresis. Each SDS-PAGE band consists of some polypeptides showing pI heterogeneity, while some polypeptides … More of same pI showed different molecular weight. The polymorphic bands in IEF were detected as each single spot, and belong to alpha-2 and beta-2. The genetic analysis of the polymorphic bands were done by IEF using "Kinmaze", "IR24" and RI (recombinant inbred) lines. "IR24" shows almost the same pattern with "IR36", but has another band in alpha subunit. RI lines were made from a cross seed between "Asominori", showing the same pattern with "Kinmaze", and "IR24". The bands of reciprocal F1 hybrid seeds between "Kinmaze"and "IR24"showed the gene dosage effect. In F2 analysis, segregation of the bands, phenotypes in staining intensity, fitted to a ratio of 1 : 1 : 1 : 1 (or 3 : 1), indicating these bands were controlled by incomplete dominant genes. Recombinants were found among the bands, indicating each band was controlled by each incomplete gene. The linkage relationship of the genes controlling each polymorphic bands were found in both genes controlling alpha-2 polypeptides and alpha-3 and beta-2 polypeptides. Then chromosomal location of these genes was studied by means of RI lines. The genes controlling alpha-2 polypeptides were shown to be linked with RFLP markers located on the chromosome 1, while The genes controlling alpha-3 and beta-2 polypeptides were shown to be linked with RFLP markers located on the chromosome 2. Less

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Kumamaru,T.: "High lysine mutants of rice." Rice Genet.Newslet.9. 80-82 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Mizuno,K.: "Alteration of the structural properties of starch components by the lack of an isoform of starch branching enzyme in rice seeds." J.Biol.Chem.268(25). 19048-19091 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 佐藤光: "タボラのコメは何故うまいか?" 東北大学遺伝生態研究センター通信. 22. 1-7 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Eggum,B.O.: "Protein quality evaluation of cooked rice of protein mutants in growing rats." Cereal Chem.71(2). 199-201 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 佐藤光: "イネ胚乳澱粉変異体の作製とその解析" 日本農芸化学会誌. 66. 1577-1580 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Satoh,H.: "New 57 kDa glutelin genes on chromosome 9 in rice." Rice Genet.Newslet.11(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Minakuchi,S.: "Polymorphism for prolamin polypeptides in cultivated rice collected from Bangladesh,Nepal and Bhutan." Rice Genet.Newslet.11(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 佐藤光: "「種子の科学-生産と利用のための基礎と応用-」 コメの貯蔵タンパク質に関する突然変異" 学会出版センター (印刷中), (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kumamaru, T., H.Sato and H.Satoh: "High lysine mutants of rice." Rice Genet.Newslet.9. 80-82 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Mizuno, K., T.Kawasaki, H., Shimada, H.Satoh, E.Kobayashi, S.Okumura, Y.Arai and T.Baba: "Alteration of the structural properties of starch components by the lack of an isoform of starch branching enzyme in rice seeds." J.Biol.Chem.268 (25). 19048-19091 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Satoh, H.: "Why has the rice from Tabora a good taste?" Newslet.Tohoku Univ.Res.Center Genet.Eco.No.22. 1-7 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Eggum, B.O., H.Satoh and B.O.Juliano: "Protein quality evaluation of cooked rice of protein mutants in growing rats." Cereal Chem.71 (2). 199-201 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Satoh, H.: "Induction and analysis of mutants for endosperm starches in rice." Nippon Nogeikagaku Kaishi. 66. 1577-1580 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Satoh, H., T.Kumamaru, S.Yoshimura and M.Ogawa: "New 57 kDa glutelin genes on chromosome 9 in rice." Rice Genet.Newslet.11 : (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Minakuchi, S., H.Satoh and T.Konishi: "Polymorphism for prolamin polypeptides in cultivated rice collected from Bangladesh, Nepal and Bhutan." Rice Genet.Newslet.11 : (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Satoh, H.: "Mutants for storage proteins in rice. In "Science of Seeds" ed.by K.Harada" Gakkai Shuppan Center (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Eggum,B.O.: "Protein quality evaluation of cooked rice of protein mutants in growing rats." Cereal Chem.71(2). 199-201 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 佐藤 光: "イネ胚乳澱粉変異体の作製とその解析" 日本農芸化学会誌. 66. 1577-1580 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Satoh,H.: "New 57 kDa glutelin genes on chromosome 9 in rice." Rice Genet.Newslet.11(印刷中). (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Minakuchi,S.: "Polymorphism for prolamin polypeptides in cultivated rice collected from Bangladesh,Nepal and Bhutan,a preliminary survey." Rice Genet.Newslet.11(印刷中). (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] KITANO,H.: "Hierarchical regulation of organ differentiation during embryogenesis in rice" The Plant Journal. 3. 607-610 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] MIZUNO,K.: "Alteration of the structural properties of starch components by the lack of an isoform of starch branching enzyme in rice seeds." J.Biol.Chem.,. 268. 19084-19091 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] FUSE,T.: "Characterization of a rice mutants having an increased susceptibility to light stress at high temperature." Physiol.Plant.,. 89. 799-804 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 佐藤光: "タボラのコメは何故うまいか?" 東北大学遺伝生態研究センター通信. 22. 1-7 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.Tamura,H.Kitano H.Satoh and Y.Nagato: "A gene profoundly affecting shoot organization in the early phase of rice development." Plant Science. 82. 91-99 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ideta,O.,A.Yoshimura,T.Matsumoto,H.Tsunematsu and N.Iwata: "Integration of conventional and RFLP linkage maps in rice.I.On chromosomes 1,2,3,and 4." Rice Genetics Newsletter. 9. (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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