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Isolation of cDNA clone of key enzymes realting sorbitol synthesis and the analysis of sugar accumulation

Research Project

Project/Area Number 04454052
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 園芸・造園学
Research InstitutionNagoya University

Principal Investigator

YAMAKI Shohei  Nagoya University, School of Agricultural Seicences, Professor, 農学部, 教授 (70210341)

Co-Investigator(Kenkyū-buntansha) KANAYAMA Yoshinori  : Nagoya University, School of Agricultural Seicences, Assistant Professor, 農学部, 助手 (10233868)
IMASEKI Hidemasa  Nagoya University, School of Agricultural Seicences, Professor, 農学部, 教授 (90023431)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1993: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1992: ¥4,100,000 (Direct Cost: ¥4,100,000)
KeywordsS6PDH / SDH / S6PDH cDNA clone / Sorbitol-6-P dehydrogenase / Sorbitol dehydrogenase / Sorbitol / Sugar accumulation
Research Abstract

Both sorbito-6-P dehydrogenase (S6PDH) and sotbitol dehydrogenase (SDH) are important as key enzymes to accumulate sugar into fruit tissue of apple. The purpose of this research is to make clear the role of lthese enzymes by purifying and characterizing them, and to clarify the regulation on gene expression of S6PDH by cloning the cDNA.
We cucceeded to isolate both enzymes through some purification steps. Especially, the isolation of S6PDH is very worth because this was firstly isolated from plant tissues. From the properties of purified enzymes, S6PDH was certified to react toward S6P synthesis and SDH toward the convertion of sorbitol into fructose in vivo. Further, we succeeded to isolate the cDNA clone of S6PDH using apple seedling. The cDNA is composed of a open reading fram of 930 bases coding 310 mino acid. The molecular weight of S6PDH was 35,000 and corresponded to the molecular weight estimated by SDS-PAGE.Next, the gene expression of S6PDH with germination of apple seeds was studied using this cDNA probe. It was suggested that the gene expression of S6PDH activity acts under some post-transcriptional or translational regulations since m-RNA of S6PDH appeared 2 days ealier than the protein synthesis of S6PDH.Infuture, it will be expected to make clear the detail mechanism of sugar accumulation into fruit throught the study of gene expression of SDH by cloning the cDNA.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Y.Kanayama: "Nucleotide sequence of a cDNA encoding NADP-sorbitol-6-phosphate dehydrogenase from apple" Plant Physiol.100. 1607-1608 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Moriguchi: "Levels and role of sucrose synthase,sucrose-phosphate synthase,and acid invertase in sucrose accumulation in fruit of Asian pear" J.Amer.Soc.Hort.Sci.117. 274-278 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Kanayama: "Purification and properties of NADP-dependent sorbitol-6-phosphate dehydrogenase from apple seedlings" Plant Cell Physiol.34. 819-823 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 金山喜則: "果実が甘くなるしくみ" 化学と生物. 31. 578-587 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Kanayama, H.Mori, H.Imaseki and S.Yamaki: "Nucleotide sequence of a cDNA encoding NADP-sorbitiol-6-phosphate dehydrogenase from apple" Plant Physiol. 100. 1607-1608 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Moriguchi, K.Abe, T.Sanada and S.Yamaki: "Levels and role of sucrose synthase, seurose-phos phate synthase, and acid invertase in sucrose accumulation in fruit of Asian pear" J.Amer.Sco.Hort.Sci.117. 274-278 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Kamayama and S.Yamaki: "Purification and properties of NADP-dependent sobitol-6-phosphate dehydrogenase from apple seedlings" Plant Cell Physiol. 31. 578-587 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Kanayama and S.Yamaki: "Mechanism o sugar accumulation in fruit" KASEAA. 31. 578-586 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Kanayama: "Purification and properties of NADP-dependent sorbitol-6-phosphate dehydrogenase from apple seedlings" Plant Cell Physiol.34. 819-823 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 金山喜則: "果実が甘くなるしくみ" 化学と生物. 31. 578-587 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.Kanayama,H.Mori,H.Imaseki & S.Yamaki: "Nucleotide sequence of a cDNA encoding NADP-sorbitol-6-phosphate dehydrogenase from apple" Plant Physiol.100. 1607-1608 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Moriguchi,K.Abe,T.Sanada & S.Yamaki: "Levels and role of sucrose synthase,sucrose-phosphate synthase,and acid invertase in sucrose accumulation in fruit of Asian pear" J.Amer.Soc.Hort.Sci.117. 274-278 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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