| Project/Area Number |
04454055
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
園芸・造園学
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| Research Institution | Okayama University |
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Principal Investigator |
INABA Akitsugu Okayama University, Fac.of Agri culture, Professor, 農学部, 教授 (90046491)
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1992: ¥4,900,000 (Direct Cost: ¥4,900,000)
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| Keywords | Ethylene biosynthesis / ACC synthase gene / Electrical impedance analysis / 膜機能変化 / ACC合成酵素 / ACC酸化酵素 / cDNA / プロトンポンプ / トノプラスト |
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| Research Abstract |
Electrophysiological studies on ethylene biosynthesis was conducted with application of direct current to cucumber (Cucumis sativus L.) fruit.
1) Direct current of 1 to 3mA induced ethylene synthesis with a sharp peak at 1h. This abrupt induction of ethylene synthesis was accompained by an equally abrupt activations of 1-aminocyclopropane-1-carboxylate (ACC) synthase and ACC oxidase.
2) At least six different cDNAs for ACC synthase were obtained from cucumber fruit. By northerm blotting, five ACC synthase mRNAs were expressed withdirect current in cucumber fruit.
3) Electrical impedance analysis of tissue properties was conducted in order to understand the relationship between impedance components and ethylene biosynthesis induced by direct current in cucumber fruit.Impedances were calculated from five components ; capacitances representing plasma membrane and organelle membranes, and resistances representing extracellular space, cytoplasm, and organelle interior. With direct current of 3 mA,capacitance of organelle membranes and resistances of extracellular space and cytoplasm were increased abruptly.
Diazocyclopentadiene, an inhibitor of ethylene action, eliminated the direct current induction of resistance of extracellular space, but no effect on the increase in capacitance of organelle membranes and resistance of cytoplasm. The results indicate that an extemally applied current may generate signal (s) by altering the functions of organelle membranes and/or cytoplasmic pH to induce ACC synthase.
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