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MICHANISMS OF SIGNAL TRANSDUCTION AND GENE EXPRESSION OF THE PLANT ACTIVE DEFENSE GENES IN PLANT MICROBE INTERACTION

Research Project

Project/Area Number 04454062
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 植物保護
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

YAMADA Tetsuji  Okayama Univ., Fac.Agri., Professor, 農学部, 教授 (00191320)

Co-Investigator(Kenkyū-buntansha) SHIRAISHI Tomonori  Okayama Univ., Fac.Agri., Professor, 農学部, 教授 (10033268)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1992: ¥5,100,000 (Direct Cost: ¥5,100,000)
KeywordsPhenylalanine ammonia-lyase / PAL / chalcone synthase / CHS / pea / elicitor / suppressor / Mycosphaerella pinodes / フェニールアラニンアンモニア・リアーゼ / 遺伝子発現制御 / ホースホイノシトール / 原形質膜
Research Abstract

The object of this research is to elucidate the mechanisms of signal transduction and control of the genes involved in plant active defense responses. We have characterized the molecular basis for the signal transduction pathway and cis- and trans-acting factors involved in the activation and deactivation of plant active defense genes, particularly focused on the study of the effects of elicitor and suppressor produced by a pea pathogen, Mycosphaerella pinodes, on the signal tansduction and the regulation of the genes encodig PAL and CHS in in pea active defense responses. Details of the current status of the studies are,
1. The phosphorylation of PIP and PIP_2 by lipid kinase was stimulated by the treatment with elicitor, but it was distinctively suppressed in the concomitant presence of suppressor. Suppressor inhibits not only PM-ATPase but also the phosphorylation of PIP and PIP_2, which suggests that ATPase and PI-, PIP-kinases cross-talk each other and blocking PM-ATPase activity r … More esults in delaying the induction of the expression of PAL and CHS in a manner very similar to a fungal suppressor.
2. Structure and gene organization of the two members of PAL gene (PSPAL1 and PSPAL2) and two members of CHS gene (PSCHS1 and PSCHS2) in a small multigene family were determined. Sequentially deleted promoter fragment of PSPAL1 and PSPAL2 connected to a plant expression vector containing the reporter gene was introduced into pea protoplasts by electroporation, and induction by elicitor and suppression by suppressor was examined. Cis- element responsible for elicitor induction in both PSPAL genes contain consensus sequence motifs of box 2 and box 4, whereas both PSCHS genes contain box 2.
3. Trans-acting factor(s) and cis-regulatory elements responsible for elicitor-induction in PSPAL1 and PSPAL2 were defined by gel retardation assay and in vitro DNAse I-footprinting analysis. At least two nuclear binding proteins were found to be involved in elicitor-induction which recognize AT rich sequence motifs. Less

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] Yamada,T.et al.: "Phenylalanine ammonia-lyase genes from Pisum sativum:Structure,organspecific expression and regulation by fungal elicitor and suppressor." Plant Cell Physiol.33. 715-725 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nasu,K.et al.: "An endogenous Suppressor of the defense response in Pisum sativum." Plant Cell Physiol.33. 617-626 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Toyoda,K.et al.: "Regulation of phosphoinositide metabolism in pea plasma membranes by elicitor and suppressor from a pea pathogen,Mycosphaerella pinodes." Plant Cell Physiology.33. 445-452 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Shiraishi,T.et al.: "Two suppressors,Suppressin A and B,secreted by a pea pathogen,Mycosphaerella pinodes." Plant Cell Physiol.33. 663-667 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] An,C.et al.: "Structure and organization of the genes encoding chalcone synthase in Pisum sativum." Plant Mol.Biol.21. 789-803 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kato,T.et al.: "Inhibition of ATPase activity in pea plasma membranes by the fungal suppressors from Mycosphaerella pinodes and the peptide moieties." Plant Cell Physiol.34. 439-445 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 山田哲治: "植物病原性 微生物研究法(脇本 哲 監修)" ソフトサイエンス社, 3 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 白石友紀、山田哲治: "植物細胞工学" 秀潤社, 7 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yamada, T.et al.: "Phenylalnine ammonia-lyase genes from Pisum sativum : Structure, organ-specific expression and regulation by fungal elicitor and suppressor." Plant Cell Physiol.33. 715-725 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nasu, K.et al.: "An endogenous Suppressor of the defense response in Pisum sativum." Plant Cell Physiol.33. 617-626 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Toyoda, K.et al.: "Regulation of phosphoinositide metabolism in pea plasma membranes by elicitor and suppressor from a pea pathogen, Mycosphaerella pinodes." Plant Cell Physiol.33. 445-452 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Shiraishi, T.et al.: "Two suppressors, Suppressin A and B, secreted by a pea pathogen, Mycosphaerella pinodes." Plant Cell Physiol.33. 663-667 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] An, C.et al.: "Structure and organization of the genes encoding chalcone synthase in Pisum sativum." Plant Mol.Biol.21. 789-803 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kato, T.et al.: "Inhibition of ATPase activity in pea plasma membranes by the fungal suppressors from Mycosphaerella pinodes and the peptide moieties." Plant Cell Physiol.34. 439-445 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiramoto, H.et al.: "The relationship between systemic resistance induced by pruning and accumulation of antifungal substances in barley seedling." J.Phytopath.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] An,C.et al.: "Structure and organization of the genes encoding chalcone synthase in Pisum sativum." Plant Mol.Biol.21. 789-803 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kato,T.et al.: "Inhibition of ATPase activity in pea plasma membranes by the fungal suppressors from Mycosphaerella pinodes and the peptide moieties." Plant Cell Physiol.34. 439-445 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Toyoda,K.et al.: "Rapid changes in polyphosphoinositide metabolism in pea in response to fungal signals." Plant Cell Physiol.34. 729-735 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamada,T.et al.: "Suppression of pisatin and phenylalanine ammonia lyase-mRNA in a compatible interaction between Pisum sativum L.cv.Midoriusui and Pseudomonas syringae pv.pisi." Ann.Phytopath.Soc.Jpn.(in press.).

    • Related Report
      1993 Annual Research Report
  • [Publications] Hiramoto,H.et al.: "Signal molecules inducing systemic resistance and susceptibility in barley seedling." J.Phytopath.(in press.).

    • Related Report
      1993 Annual Research Report
  • [Publications] Hiramoto,H.et al.: "The relationship between systemic resistance induced by pruning and accumulation of antifungal substances in barley seedling." J.Phytopath.(in press.).

    • Related Report
      1993 Annual Research Report
  • [Publications] Shiraishi,T.et al.: "Host-Specific Toxin:Biosynthesis,Receptor and Molecular Biology" Tottori Univ.Press/Tottori (in press.),

    • Related Report
      1993 Annual Research Report
  • [Publications] 白石友紀、山田哲治: "植物細胞工学" 秀潤者, 7 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamada,T.: "Phenylalanine ammoniea-lyase genes from Pisum sativum:Structure.organ-specific expression and regulation by fungal elicitor and suppressor." Plant Cell Physiol.33. 715-725 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kawamala,S.: "Molecular cloning of phenylalanine ammonia-lyase cDNA form Pisum sativum." Plant Mol.Biol.20. 167-170 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ichinose,Y.: "Molecular cloning of chalcone synthase cDNAs from Pisum sativum." Plant Mol.Biol.18. 1009-1012 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hashimoto,T.: "Transient expression of phenylalanine ammonia-lyase promoter in electroporated pea protoplasts." Plant Cell Reports.11. 183-187 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Shiraishi,T.: "Two suppressors,Suppressin A and B,secrdted by a pea pathogen,Mycosphaerella penodes." Plant Cell Physiol.33. 663-667 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] An,S.: "Structure and organization of the genes encoding chalcone synthase in Pisum sativum." Plant Mol,Biol.

    • Related Report
      1992 Annual Research Report
  • [Publications] 山田 哲治: "微生物研究法" ソフトサイエンス社, (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 山田 哲治: "病原菌の生産するエリシターとサプレッサーによる植物防御反応と遺伝子発現調節" 化学と生物(学会出版センター), 3 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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