| Project/Area Number |
04454075
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用微生物学・発酵学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
HORINOUCHI Sueharu Univ.Tokyo, Dept.Agric.A.Prof., 農学部, 助教授 (80143410)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIYAMA Makoto Univ.Tokyo, Dept.Agric.Assistant Prof., 農学部, 助手 (00208240)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1993: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1992: ¥4,200,000 (Direct Cost: ¥4,200,000)
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| Keywords | Streptomyces / A-factor / Antibiotic production / morphogenesis / Signal transduction / Protein phosphorylation |
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| Research Abstract |
1. A-factor is a microbial hormone controlling streptomycin production and sporulation. An A-factor-dependent promoter, the strR promoter, in the streptomycin biosynthetic gene cluster of S.griseus was identified. Gel retardation assays detected a protein that specifically bound the strR promoter.
2. The AfsR protein is essential for the biosynthesis of A-factor, actinorhodin, and undecylprodigiosin in S.coelicolor. The AfsR protein was purified to homogeneity and found to be phosphorylated by a specific kinase. It is conceivable that the extent of phosphorylation of AfsR modulates its regulatory activity which, in turn, regulates expression of some target genes involved in secondary metabolism in this strain.
3. In vitro phosphorylation reactions using extracts of S.griseus cells revealed the presence of multiple phosphorylated proteins. Most of these phosphorylations were distinctly inhibited by staurosporine and K-252a which are known to be eucaryotic protein kinase inhibitors. Consistent with this, these kinase inhibitors inhibited antibiotic production and aerial mycelium formation in S.griseus and S.coelicolor.
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