| Project/Area Number |
04454112
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
基礎獣医学
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| Research Institution | Hokkaido University |
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Principal Investigator |
ITAKURA Chitoshi Hokkaido University, Dept. of Comparative Pathology, Professor, 獣医学部, 教授 (30021695)
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| Co-Investigator(Kenkyū-buntansha) |
OCHIAI Kenji Hokkaido University, Dept. of Comparative Pathology, Lecturer, 獣医学部, 講師 (80214162)
KIDA Hiroshi Hokkaido University, Dept. of Microbiology, Associate Professor, 獣医学部, 助教授 (10109506)
御領 政信 北海道大学, 獣医学部, 助教授 (80153774)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1993: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1992: ¥4,400,000 (Direct Cost: ¥4,400,000)
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| Keywords | Calicivirus / Rabbit hemorrhagic disease / Virus antigen / Immunostain / Virus particles / Hepatitis / 壊死性肝炎 / 播種性血管内凝固 / ウイルス複製 / 白血球毒性 / 肝臓 |
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| Research Abstract |
A rabbit hemorrhageic disease (RHD) virus was isolated from Korean rabbits. The purified virus particles were 35 to 40 nm in diameter and had hollow depressions on their surface. The structural protein of the virus was composed of a single major polypeptide of 63 kDa.
This virus had the high virulence and caused acute deaths in rabbits. Histopathological lesions caused by RHD virus were acute necrotizing hepatitis consisting of focal or solitary necrosis of hepatocytes.
Immunohistochemically, RHD virus antigens were detected in degenrated and necrotic hepatocytes in formalin fixed tissues from rabbits infected with RHD virus. The findings indicated that RHD virus has a predilection for hepatocytes and replicates in the cytoplasm of the hepatocytes. The virus antigens were also detected in the endothelial cells and macrophages of the spleen.
Ultrastructurally, a large number of virus particles resembling a calicivirus in size and morphology were demonstrated in the cytoplasm of many necrotic hepatocytes. The particles were accumulated mainly in the vaculoes of the necrotic hepatocytes.
We isolated a virus from the liver of RHD in China. The characteristics and pathogenicity of the virus were the same as those of the Korean virus.
From the results obtained here, the calicivirus isolated from RHD was hugh virulent and had an affinity faor adult rabbit hepatocytes.
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