| Project/Area Number |
04454133
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Kanazawa Medical University |
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Principal Investigator |
ONODA Norihiko Kanazawa Medical University. Department of Physiology, Professor, 医学部, 教授 (60106903)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1993: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1992: ¥5,100,000 (Direct Cost: ¥5,100,000)
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| Keywords | CAT gene / virus producing cell line / Rat / olfactory bulbectomy / viral infection / psi^- / lac Z gene |
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| Research Abstract |
A pZip-Neo-CAT(chlormaphenical acetyl transferase) vector was transformed into a psi^- cell line which lacked a packaging signal. The psi^- cell line with the CAT gene was segregated by G418 (neamycin analogue) and its CAT activity was checked. A virus producing cell line (8-7) was established. Viruses with the foreigen genes were harvested by culturing the virus producig cells.
The adult rats were used. Under anesthesia the olfactory bulb was removed. In four days after olfactory bulbectomy (OBx), animals were transfected with the CAT-introduced viruses. One week after viral transfection, animals were fixed with 4% paraformaldehyde. Frozen flontal sections were cut. The sections were incubated with an antibody against CAT.CAT-positive olfactory sensory neurons were not found in OBx rats. Titers of the virus producing cell line might not be high enough or infection force of virus env gene would be too weak.
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