| Project/Area Number |
04454142
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | NATIONAL INSTITUTE FOR PHYSIOLOGICAL SCIENCES |
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Principal Investigator |
OBATA Kunihiko NATL.INST.PHYSIOL.SCI., PROF., 生理学研究所, 教授 (60013976)
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| Co-Investigator(Kenkyū-buntansha) |
HASHIMOTO Takanori NATL.INST.PHYSIOL.SCI., RESEARCH ASSOCIATE, 生理学研究所, 助手 (40249959)
KOJIMA Nobuhiko NATL.INST.PHYSIOL.SCI., RESEARCH ASSOCIATE, 生理学研究所, 助手 (80215251)
YAMAGATA Yoko NATL.INST.PHYSIOL.SCI., RESEARCH ASSOCIATE, 生理学研究所, 助手 (20210338)
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1993: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1992: ¥4,100,000 (Direct Cost: ¥4,100,000)
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| Keywords | Hippocampus / neuronal plasticity / kindling / kainic acid / molecular cloning / protein phosphorylation / long-term potentiation / synapsin I / チトクロム・オキシダーゼ / ミトコンドリアDNA |
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| Research Abstract |
In order to elucidate the molecular mechanism of neuronal plasticity, we investigated specific changes in gene expression and protein phosphorylation of the rat hippocampus after kindling or long-term potentiation was induced. Molecular cloning was performed on the subtracted cDNA library prepared from kindled and control hippocampi. Expression of genes for cytochrome oxidase subunits 2 and 3 was elevated by kindling. Protein phosphorylation by calcium/calmodulin-dependent kinase II was investigated on the hippocampus and cerebral cortex after kindling. Although enzyme activity was not altered significantly, phosphorylation of synapsin I was elevated in kindled hippocampus and cortex. Glutamate release from synaptosome fraction was investigated in vitro. High potassium stimulation induced the more release from kindled cortex.
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