| Project/Area Number |
04454180
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Human pathology
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| Research Institution | Kyushu University |
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Principal Investigator |
SUEISHI Katsuo Kyushu University, Faculty of Medisine, Professor, 医学部, 教授 (70108710)
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| Co-Investigator(Kenkyū-buntansha) |
KONO Shinji Kyushu University, Faculty of Medicine, Assistant, 医学部, 助手 (20225379)
NAKAGAWA Kazunori Kyushu University, Faculty of Medicine, Assistant, 医学部, 助手 (50217668)
NAKASHIMA Yutaka Kyushu University, Faculty of Medicine, Assistant Professor, 医学部, 助教授 (50135349)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1993: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1992: ¥6,300,000 (Direct Cost: ¥6,300,000)
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| Keywords | Thrombus / Atherosclerosis / Angiogenesis / Endothelial cells / Macrophages / Tissue factor / IL-1 / IL-1ra / IL‐1 / IL‐1レセプターアンタゴニスト |
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| Research Abstract |
The Interaction between blood and vascular wall is dynamic and endothelial cells play a centralrole in the regulation of its interaction and the maintenance of vascular function. In this projectwe investigated the role of endothelial function in the development of thrombosis, atherosclerosis and angiogenesis. The main results obtained in this project are as follows.
1. Endothelial function in thrombogenesis : In rabbits with Shwartzman reaction, 1)tissue factor(TF) was immunohistochemically expressed by not only Kupffer cells but endothelial cells, 2)this expression of TF seemed to be partly regulated by the expression of IL-1 beta ra by Kupffer cells, 3)the TF expression by endothelial cells was noted at abluminal side as well as luminal surface. Inaddition, the localization of TF mRNA was also observed by in situ hybridization using human TF cDNAprobes.
2. Regulation mechanism of angiogenesis : We clarified that the angiogenic induction was enhanced by hypoxia, which stimulated glial cells and cancer cells to release VEGF and bFGF.Re-oxigenation of hypoxic endothelials also up-regulated the receptors for VEGF and bFGF on the endothelial surface.
3. Endothelial function in the atherosclerotic progression : In the patients with inflammatory abdominal aortic aneurysm, which generally follows abdominal aortic atherosclerosis, IE genome of human cytomegalovirus wad strongly expressed by endothelial cells of vasa vasora as well as macrophages and fibroblast-like mesen-chamal cells and seemed to participate essentially in the progression of its inflammatory process.
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