| Project/Area Number |
04454186
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Niigata University |
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Principal Investigator |
KIHARA Itaru Niigata Univ.School of Med.Professor, 医学部, 教授 (80018324)
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| Co-Investigator(Kenkyū-buntansha) |
KAWASAKI Katsustoshi the same above Instructor, 医学部, 講師 (20152944)
YAMAMOTO Tadashi the same above Assistant Professor, 医学部, 助教授 (30092737)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1992: ¥5,500,000 (Direct Cost: ¥5,500,000)
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| Keywords | Visceral epithelial cell / Slit-diaphragm / Cell cycle / Autoradiography / Glomerular adhesion / Monoclonal antibody / Micropuncture / Glomerular filtration rate / 尿蛋白排泄 / 心房性ナトリウム利尿ペプチド / 単クローン抗体 |
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| Research Abstract |
1.The monoclonal antibody, 5-1-6 reacts with an unknown antigen that locates near slit diaphragms of the rat glomerular visceral epithelial cell (VEC) and produces proteinuira upon a single intravenous injection. The model provides mechanism water and electrolytes are filtrated through the glomerular basement membrane or slit-diaphragm. After 2 and 24 h after the 5-1-6 injection single nephron glomerular filtration rate (SNGFR) and other parameters related glomerular filtration coefficient (Kf) were obtained by micropuncture. As results, Kf is reduced to 50% of the normal level at 2 h, against higher values of either glomerular capillary pressure or hydraulic pressure, and normal SNGFR.Kf returns normal by 24 h inspite of higher capillary pressure and increased SNGFR.In morphometry volume rations of the VEC increased by 2 h. The numbers of foot processes per unit length of the GBM and the distance of slit diaphragms should be pursued.
2.Normal VEC belongs to the highly developed epithelial cell that duplicates seldomly. Detachmnent and separation from the GBM are critical to glomerular function and morphology. Glomerular adhesions are analyzed by light and electron successive against VEC and autoradiography. In results, detachment of VEC resulted through irreversible VEC alteration. We conclude that focal and segmental VEC detachment from the GBM as well as confrontation with hypertrophic parietal epithelial cell are prerequisites for the development of glomerular adhesion.
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