| Project/Area Number |
04454201
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | Osaka University |
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Principal Investigator |
UEDA Shigeharu RESEARCH INSTITUTE FOR MICROBIAL DISEASES,OSAKA UNIVERSITY DEPARTMENT OF NEUROVIROLOGY PROFESSOR, 微生物病研究所, 教授 (90068453)
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1994: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1993: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1992: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | measles / measles vaccine / measles virus / H protein / F protein / antigenic diversity / amino acid sequence / アミノ酸配列 / 赤血球凝集素 / 糖鎖 / 単クローン抗体 / 抗原性変異 / 溶血素 |
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| Research Abstract |
Some children suffered from measles illenss several years after vaccination with live attenuated measles virus vaccines. We investigated differences in antigenicities between vaccine strains and current strains of measles virus.
1) Antibody responses of children after revaccination
Eleven children were revaccinated with CAM measles vaccine 10 years after previous vaccination. Serum samples were obtained 2 weeks later. Five children showed 8 times or higher antibody titers against the homologous CAM vaccine strain than a current strain (the F-b strain). The lowest antibody titer, however, was 2^<7.0> against both challenge strains. The results suggest some minor antigenic differences between the vaccine strain and the current strain.
Amino acid sequences of H and F proteins of measles virus
H and F genes of the CAM vaccine strain and the F-b strain were sequenced by the method of PCR direct sequencing. Amino acid sequences were deduced from the nucleotide sequences both genes. Twenty six substitutions were detected in H proteins between the vaccine and current strains and only 7 substitutions in F protein.
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