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The study of the production of human antibody in vitro expression system and recognition of epitope on the HA protein by human immune system.

Research Project

Project/Area Number 04454202
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionNagoya City University

Principal Investigator

NAKAJIMA Katsuhisa  Nagoya City University School of medicine, professor, 医学部, 教授 (40012778)

Co-Investigator(Kenkyū-buntansha) NAKJIMA Setsuko  The lnstitute of Public Health Department of Microbiology, Section Chief, 微生物学部, 室長 (80124402)
NOBUSAWA Eri  Nagoya City University School of medicine, lecturer, 医学部, 講師 (90183904)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1993: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1992: ¥3,600,000 (Direct Cost: ¥3,600,000)
KeywordsInfluenza Virus / hemagglutinin / monoclonal antibody / in vitro antibody synthesis / in vitro発現
Research Abstract

Influenza A virus is unique among human viruses in its capacity to alter the antigenic phenotype with relative ease and evade neutralizing antibodies. This property has been occurred with a small amount amino acid changes. Mous monoclonal antibody study revealed that many epitopes exist on the HA protein Why the small amino acid change on the HA protein help to evade human neutralizing antibodies. To understand the reason, we should analyze the huma immune-system for HA protein of influenza A virus. We studied two system. On is the approach to produce human monoclonal antibodies by in vitro expression system. Second one is to analyze human serum with epitope scanning system. For the development of the system to produce human monoclonal antibody, we tried to produce mouse monoclonal antibodies for influenza HA protein with using mouse hybridoma mRNA.Immunozap expression vector system (STRATAGENE Co.) did not work. We did not identify the production of an antibody in E.Coli. Recently, single strand antibody system has developed. We used this system. Antibody production was detected with western blot. The specificity of this antibody was weak to identify the HA protein. However, this system seems to be useful for in vitro antibody production with some modification. Epitope scanning methods indicated that 10 amino acid peptide identified some epitopes but still remained some ambiguous. So we are now trying 12 amino acid system.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 中島捷久・中島節子: "インフルエンザウイルスの抗原変換と生体防御" 生体防御. 9. 147-153 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 中島節子・西川文雄・中村和幸・中島捷久: "Comparison of the HA gene of type B infwenza viruses in herald waves and later epidemic seasons" Epidemiology and Infection. 109. 559-568 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 中島節子・西川文雄・中村和幸・中尾春寿・中島捷久: "Reiafection with influenza B virus in children" Epidemiology and Infection. 113. 103-112 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 中島捷久・信澤枝里・中島節子: "小児医学の進歩´92「インフルエンザウイルス変異株出現のメカニズム」" 中山書店, (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Nakajima, F.Nishikawa, K.Nakamura and K.Nakajima: "Comparison of the HA genes of type B influenza viruses in herald waves and later epidemic seasons" Epidemiol.Infect.109. 559-568 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Ochiai, Akesue, M.Kurokawa, K.Nakajima and H.Nakagawa.: "Enhanced production of rat interleukin-8 by in Vitro and in Vivo infections with influenza A NWS virus" J.Virol. 67. 6811-6814 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Nakajima, F.Nishikawa, K.Nakamura, H.Nakao and K.nakajima: "Reinfection with influenza B virus in children : analysis of the reinfection influenza B viruses." Epidemiol.Infect.113. 103-112 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 中島節子・西川文雄・中村和幸・中島捷久: "Reinfection with influenza B virus in children" Epidemiology and Infection. 113. 103-112 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 中島 節子: "Comparison of the HA genes of type B influgnza viruses in herald waves and later epidemic seasons" Epidemiology and Infection. 109. 559-568 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 中島 捷久: "小児医学の進歩'92A" 中山書店, 345 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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