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THE SYUDY OF SIGNAL TRANSDUCTION IN DISEASED HEART AND MOLECULAR CLONING OF NEW G PROTEIN.

Research Project

Project/Area Number 04454259
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Circulatory organs internal medicine
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

KAWAGUCHI Hideki  HOKKAIDO UNIVERSITY MEDICAL HOSPITAL,LECTURE, 医学部・附属病院, 講師 (70161297)

Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1993: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1992: ¥5,000,000 (Direct Cost: ¥5,000,000)
KeywordsGTP BINDING PROTEIN / HYPERTROPHIC CARDIOMYOPATHY / DCM / SIGNAL TRANSDUCTION / alpha ADRENOCEPTOR / beta ADRENOCEPTOR / G蛋白質 / 心筋症 / 心筋症モデル動物 / PLC / 1P3
Research Abstract

Free beta gamma subunits of GTP binding protein can themselves interact functionally with protein. Recent results suggest that beta subunit might be playing a key role to specify the receptor-effector coupling in various transmembrane signaling systems. The purpose of this study is to examine the expression level and distribution of G protein beta subunit in rat hearts.
Brain and ventricle of WKY rats were used to examined the expression of three different isoforms of beta subunits in mRNA and protein level by means of Northern bolt and Western bolt analysis, respectively.
beta3 mRNA was expressed significantly more in ventricles than in brains. This result was supported by Western bolt analysis showing that beta3 was expressed more in ventricles. For all three isoforms, the majority of beta subunits expressed in rats hearts were detected in cytosolic fraction. An ultrafiltration and susequent Western bolt analysis revealed that the beta subunits in ventricular cytosolic fractions had molecular mass greater than 100,000 dalton.
In this study, tissue-specific expression of beta subunits isoforms were revealed in rat hearts.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] Hideaki Kawaguchi: "Increased calcium release from sarcoplasmic reticalam Stimulated by inositol trisposphate in spontaneously hypentensive rat heart cells" Mol Cell Biochem. 119. 51-57 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Wataru Sato: "Deletion of mitochondrial DNA in a patient with condaction block" Am heart J. 125. 550-552 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hideaki Kawaguchi: "Phosphatidylinositol Metabolism in hypertrophic rat heart" Cin Res. 72. 966-972 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kenji Iizuka: "Effect of thiol protease inhibitors on fordrin degradotion daring hypoxia in caltured myocytes" J Mol Cell Candiol. 25. 1101-1109 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroshi Okamoto: "Molecualar dynamics of the phospholipid bilayer in cardiomyopathic hamster heart cell membrane" J Mol Cell Candiol. in press

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kenji Iizuka: "Trartorming Growth Factor bete-1 Modulates the number of Beta Adrenergic Receptor in Candiac Fibroblasts" J Mole Cell Candiol. in press

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hideaki Kawaguchi: "The Candiomyopathic heart" Eds M.Nagano,N.Takeda and N.S.Dhalla Raven Press Press, 464 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hideaki Kawaguchi: "Cellular function and metabolism" Eds Y.Yazaki and S.Mochizuki Kluwe Academic Pablishers, 220 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hideki Kawaguchi: "Increased calcium release from sarcoplasmic reticulum stimulated by inositol trisphosphate in spontaneously hyper tensive rat heart cells" Mol Cell Biochem. 119. 51-57 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Wataru Sato: "Deletion of mitochondrial DNA in a patient with conduction block" Am heart J. 125. 550-552 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hideki Kawaguchi: "Phosphatidylinositol Metabolism in hypertrophic rat heart" Cir Res. 72. 966-972 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kenji Iizuka: "Effect of thiol protease inhibitors on fordrin degradation during hypoxia in cultured myocytes" J Mol Cell Cardiol. 25. 1101-1109 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroshi Okamoto: "Molecular dynamics of the phospholipid bilayr in cardiomyopathic hamster heart cell membrane" J Mol Cell Cardiol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kenji Iizuka: "Tranforming Growth Factor beta-1 Modulates the number of Beta Adrenergic Receptor in Cardiac Fibroblasts" J Mol Cell Cardiol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hideki Kawaguchi: The Cardiomyopathic heart. Eds M.Nagano, N.Takeda and N.S.Dhalla. Raven Press Press, 105-114 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hideki Kawaguchi: Cellular function and metabolism Eds Y.Yazaki and S.Mochizuki. Kluwe Academic Publishers, 51-58 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hideaki Kawaguchi: "Increased calcium release from sarcoplasmic reticulum stimulated by inositol trisphosphate in spontaneously hypertensive rat heart cells" Mol Cell Biochem. 119. 51-57 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Wataru Sato: "Deletion of mitochondrial DNA in a partient with conduction block" Am Heart J. 125. 550-552 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hideaki Kawaguchi: "Phosphatidylinositol Metabolism in hypertropnic rat heart" Cir Res. 72. 966-972 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kenji Iizuka: "Effect of thiol protease inhibitors on fordrin degradation during hypoxia in caltured myocytes" J Mol Cell Cardiol. 25. 1101-1109 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hiroshi Okamoto: "Molecular dynamics of the phospholipid bilayer in cardiomyopathic hamster heart cell membrane" J Mol Cell Cardiol. (in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] Kenji Iizuka: "Tranforming Growth Factor beta-1 Modulates the number of Beta Adrenergic Receptor in Candiac Fibroblasts" J Mol Cell Cardiol. (in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] Hideaki Kawaguchi: "The Cardiomyopathic heart" Eds M Nagano,N.Takeda and N.S.Dhalla Raven Press Press, 464 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hideaki Kawaguchi: "Cellular function and metabolism" Eds Y.Yazaki and S.Mochizuki Kluwe Academic Publishers, 220 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hideaki Kawaguchi: "The studies of the cell damage and cell proliferation factors which induce cardiomyopathy." Jpn.Circ.J.56. 1037-1044 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kazushi Urasawa: "The mechanism of cathecholaminーtolerance in heart failure:the change of Adenylyl cyclase system" Jpn.Circ.J.56. 456-461 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Mikako Shoki: "Phosphatidylinositol metabolism in hypertrophic rat heart" Jap.Circ.J.56. 142-147 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kenji Iizuka: "Effect of hypoxia on calcium activated neutral protease activity in myocytes." Jpn.Heart J.33. 707-715 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hirofumi Sawa: "Expression of the angiotensinogen gene and localization of its protein in the human heart" Circulation. 86. 138-146 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hideaki Kawaguchi: "Inositolphosphatides metabolism in hypertrophic rat heart" J.Mol.Cell Cardiol.24. 1003-1010 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hideaki Kawaguchi: "PI response and calcium overload in cardiomyopathic hamster heart cells. New Aspect in the Treatment of Failing Heart" SpringerーVerlag, 5(36-40) (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kazushi Urasawa: "A mechanism of cathecholamin Tolerance in congestive heart failure New Aspect in the Treatment of Failing Heart" SpringerーVerlag, 6(75-80) (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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