• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Effects of LDL on SMC Proliferation and Intracellular Signalings

Research Project

Project/Area Number 04454274
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Circulatory organs internal medicine
Research InstitutionUniversity of Occupational and Environmental Health, Japan

Principal Investigator

NAKASHIMA Yasuhide  UOEH,School of Medicine, Associate professor, 医学部, 助教授 (20038780)

Co-Investigator(Kenkyū-buntansha) SUGANO Masahiro  UOEH,School of Medicine, Asistant professor, 医学部, 助手 (20206395)
TAKAHARA Kazuo  UOEH,School of Medicine, Lecturer, 医学部, 講師 (30163306)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 1993: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1992: ¥3,100,000 (Direct Cost: ¥3,100,000)
KeywordsLow-density lipoprotein / arterial smooth muscle cell / Intracellular Ca^<2+> / free cholesterol / フリーコレステロール / 動脈硬化退縮 / プロブコール / ヂルチアゼム / イノシトール三リン酸 / LDL / SMC / Ca^<++>
Research Abstract

To elucidate the mechanism of low-density lipoprotein (LDL) from familial hypercholesterolemic (FH) human subjects on the cultured smooth muscle cell (SMC), cell proliferation, Ca2+ movements and membrane structure were examined, along with the antiproliferative action of heparin or sulfated glycosaminoglycans. Cell growth as well as Ca^<2+> uptake was stimulated in a concentration-dependent fashion with FH-LDL compared to control (without LDL). With methylated LDL, which had no capacity to bind LDL receptor, the proliferation and Ca2+ uptake were still significantly increased compared to control. But the about 60% of increase over control was reduced by methylation. In analysis of lipid composition, the exposure to FH-LDL elevated free cholesterol contents in SMC membrane. In structural analysis, FH-LDL brought the swelling in the intrabilayr and interbilayr structure compared to control, which well correlated with change in the increase in free cholesterol in plasma membrane. Heparin or sulfated glycosaminoglycans inhibited the cell proliferation with reduction of free cholesterol in plasma membrane. We concluded that FH-LDL might increase the free cholesterol composition in plasma membrane through LDL receptor-independent pathway, and that change structure of membrane, which affect Ca2+ channel function.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] H.Tasaki、K.Yamashita、Y.Nakashima、et al: "LDL from Familial Hypercholesterolemic Subjects Promotes Arterial Smooth Muscle Cell Proliferation" Journal of Japan Atherosclerosis Society. (In press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Tasaki、K.Yamashita、Y.Nakashima、et al: "Increase of Intracellular Calcium Ion in Smooth Muscle Cell Induced by Low-Density Lipoprotein" Gerontrogy. (In press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] R.Kouzuma、H.Tasaki、Y.Nakashima、et al: "Combined Treatment of Probucol with Diltiazem Regresses Althrosclerosis Induced by 1% Cholesterol-diet in Rabbit Aorta" Arteriosclerosis and Thrombosis. (Submitting).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Tasaki, K.Yamashita, Y.Nakashima, et al: "LDL from Familial Hypercholesterolemic Subjects Promotes Arterial Smooth Muscle Cell Proliferation" Journal of Japan Atherosclerosis Society. (In press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Tasaki, K.Yamashita, Y.Nakashima, et al: "Increase of Intracellular Calcium Ion in Smooth Muscle Cell Induced by Low-Density Lipoprotein" Gerontrogy. (In press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] R.Kouzuma, H.Tasaki, Y.Nakashima, et al: "Combined Treatment of Probucol with Diltiazem Regresses Althrosclerosis Induced by 1% Cholesterol-diet in Rabbit Aorta" Arteriosclerosis and Thrombosis. (Submitting).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Tasaki,K.Yamashita Y.Naiashima et al: "LDL from Familial Hypercholesterolemic Subjects Promotes Arteria Smooth Muscle Cell Proliferation." Journal of Japan Atherosclerosis Society. In press.

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Tasaki,K.Yamashita Y.Nakashima,et al: "Increase of Intracellular Calcium Ion in Smooth Muscle Cell Induced by Low-Density Lipoprotein." Gerontrogy. In press.

    • Related Report
      1993 Annual Research Report
  • [Publications] R.Kouzuma,H.Tasaki Y.Nakashima,et al: "Combined Treatment of Probucol with Dilfiazem Regresses Althrosclerosis Induced by 1%Cholesterol-diet in Rabbit Aorta." Arteriosclerosis and Thrombosis. Submitting.

    • Related Report
      1993 Annual Research Report

URL: 

Published: 1992-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi