• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular genetic analysis of high risk factors developing childhood cancers and its clinical applications

Research Project

Project/Area Number 04454276
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionUniversity of Tokyo Hospital, Faculty of Medicine, University of Tokyo

Principal Investigator

BESSHO Fumio  University of Tokyo Hospital, Faculty of Medicine, University of Tokyo, Associate Professor, 医学部(病), 助教授 (40010285)

Co-Investigator(Kenkyū-buntansha) HIRAI Hisamaru  University of Tokyo Hospital, Faculty of Medicine, University of Tokyo, Assistan, 医学部(病), 講師 (90181130)
KAMOSHITA Shigehiko  University of Tokyo Hospital, Faculty of Medicine, University of Tokyo, Professo, 医学部(病), 教授 (60048973)
HAYASHI Yasuhide  University of Tokyo Hospital, Faculty of Medicine, University of Tokyo, Assistan, 医学部(病), 講師 (30238133)
小島 美由紀 (小林 美由紀)  東京大学, 医学部(病), 助手 (60205391)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1994: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1992: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsCancer high risk / tumor suppressor gene / p53 gene / p16 gene / p15 gene / epidemilogy / cancer clustering / childhood cancer / Rb遺伝子 / DCC遺伝子 / DAN遺伝子 / 神経芽腫 / homozygous deletion / 高危険体質 / 癌抑制遺伝子 / P53遺伝子 / RB遺伝子 / 多重がん / 点突然変異 / 小児固型腫瘍 / 小児白血病 / がん発生体質 / t(li19) / ユーイング肉腫 / 細胞株
Research Abstract

Several anti-oncogenes were studied for common childrhood cancers. Anti-oncogenes studied included p53 gene, p16/15 genes, Rb gene, DCC gene and NF1 gene. Generally, these genes tend to be mutated or deleted in cell lines more often than fresh tumor cells and in tumor cells of advanced stages and of relapsed cases. However, frequency of abnormalities of these genes varied among different tumor types. In spite of neural crest origin of both tumors, abnormalities of p53 genes were frequent seen in Ewing sarcoma but not in neuroblastoma. Abnormalities of p16/p15 genes were more frequently seen in common ALL antigen-positive acute lymphoblatic leukemia (ALL) without chromosome transloction, t (1 ; 19), than in ALL with this translocation. These abnormalies were also commn in T-ALL.
Materials of non-Hodgkin lymphoma developed in 3 siblings and lung cancer in 11-year-old boy were negative for abnormalities of p53 gene. In these patients other tumor suppressor genes might be responsible for familial or rare development of their cancers.
In addition to molecular study, epidemiological study was performed to find out familial clustering of common childhood cancers and constitutional factors affecting development of these cancers, using data of Japan Children's Cancer Registry (JCCR). We found that distribution of tumor types among family of children with cancer was different from that of general population. Brain tumor was more often seen in family of cancer, but colon cancer, lung cancer and cancer of bile duct were less often seen. Distribution of cancer types among parents and siblings whose family had cancers in 3 consecutive generations was different from cancer types of general population only in female and breast cancer and uterus cancer were more frequently seen.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Kojima R,Mizuguchi M.Bessho F,Oka T,Watababe H,Yonezawa M,Asano N,Iwanaka T: "Pulmonary carcinoma associated with hamartoma in an 11year old boy" Am J Pediatr Hematol/Oncol. 15. 439-442 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Komuro H,Hayashi Y,Kawamura M,Hayashi K,Kaneko Y,Kamoshita S,Hanada R,Yamamoto K,K,Hongo T,Yamada M,Tsuchida Y: "Mutations of the p53 gene are involved in Ewing's sarcomas but not in neuroblastoma" Cancer Res. 53. 5284-5288 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 別所文雄: "小児の症候群.Aniridia-Wilms腫瘍症候群" 小児科診療. 56. 33 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 別所文雄: "小児の症候群.Fanconi貧血" 小児科診療. 56. 87 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 別所文雄: "Fanconi貧血を巡る諸問題" 日本小児血液学会雑誌. 7. 105-116 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 林泰秀,小室広昭,川村眞智子,大西宏明,井田孔明,森脇浩一,別所文雄,鴨下重彦,土田嘉昭,花田良二,山本圭子,山田正夫: "p53遺伝子の点突然変異とN-myc遺伝子の増幅がみられた神経芽腫" 小児がん. 31. 329-332 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 林泰秀: "遺伝性造血器腫瘍" 日本臨床. 53. 2737-2741 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 林泰秀: "小児悪性腫瘍におけるp53遺伝子の意義" 医学のあゆみ. 173. 231-234 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura M,Kikuchi A,Kobayashi S,Hanada R,Yamamoto K,Horibe K,Shikano T,Ueda K,Hayashi K,Sekiya T,Hayashi Y: "Mutation of p53 and ras genes in childhood t (1;19)-acute lymphoblastic leukemia" Blood. 85. 2546-2552 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ohnishi H,Kawamura M,Hanada R,Kaneko Y,Tsunoda Y,Hongo T,Bessho F,Yokomori K,Hayashi Y: "Infrequent mutatuions of the TP53 gene and no amplification of MDM2 gene in hepatoblastomas" Genes Chromosomes Cancer. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Bessho F: "Preparation and testing of biological specimens. In : Neoplastic Disease in Childhood,edited by Pochedly C" Harwood Academic Publishier : Chur,Switzerland, 123-136 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kojima R,Mizuguchi M,BesshoF,Oka T,Watababe H,Yonezawa M,Asano N.Iwanaka T: "Pulmonary carcinoma associated with hamartoma in an 11 year old boy" Am J Pediatr Hematol/Oncol. 15. 439-442 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Komuro H,Hayashi Y,Kawamura M,Hayashi K,Kaneko Y,Kamoshita S,Hanada R,Yamamoto K,Hongo T,Yamada M,Tsuchida Y: "Mutations of the p53 gene are involved in Ewing's sarcomas but not in neuroblastoma" Cancer Res. 53. 5284-5288 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Bessho F: "Aniridia-Wilms tumor syndrome" J Pediatr Pract. 56 (Suppl). 33 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Bessho F: "Fanconi anemia" J Pediatr Pract. 56 (Suppl). 33 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Bessho F: "Unresolved problems of Fanconi's anema. (in Japanese with English abstract)" Jpn J Pediatr Heamatol. 7. 105-116 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hayashi Y,Komuro H,Kawamura M,Ohnishi H,Ida K,Moriwaki K,Bessho F,Kamoshita S,Tsuchida Y,Hanada R,Yamamoto K,Yamada M: "A child with neuroblastoma wirh a point mutation of p53 gene and N-myc gene amplification. (in Japanese)" Pediatr Oncol. 31. 329-332 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hayashi Y: "Hereditary hematopoietic malignancy with emphasis of juvenile chronic myelogenous leukemia and 7 monosomy syndrome. (in Japanese with English abstract)" Jpn J Clin Med. 53. 2737-2741 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hayashi Y: "Significance of p53 gene in childhood cancer. (in Japanese)" J Clin Exp Med. 173. 231-234 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura M,Kikuchi A,Kobayashi S,Hanada R,Yamamoto K,Horibe K,Shikano T,Ueda K,Hayashi K,Sekiya T,Hayashi Y: "Mutations of p53 and ras genes in childhood t (1 ; 19) -acute lymphoblastic leukemia" Blood. 85. 2546-2552 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ohnishi H,Kawamura M,Hanada R,Kaneko Y,Tsunoda Y,Hongo T,Bessho F,Yokomori K,Hayashi Y: "Infrequent mutatuions of the TP53 gene and no amplification of MDM2 gene in hepatoblastomas" Genes Chromosomes Cancer. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Bessho F: Preparation and testing of biological specimens. In : Neoplastic Disease in Childhood, edited by Pochedly C.Harwood Academic Publishier : Chur, Switzerland, 123-136 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 小林茂俊,他: "mRNA検出による小児悪性腫瘍におけるtyrosine hydroxylase発現の検討" 医学のあゆみ. 165. 911-912 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] 林泰秀,他: "Tyrosine hydroxy lase mRNAとt(11;22)-キメラmRNAを用いた神経芽腫とEwing肉腫の「遺伝子診断" 小児がん. 30. 337-340 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] 林泰秀,他: "小児急性リンパ性白血病におけるELA遺伝子の検討" 臨床血液. 35. 9-13 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 井田孔明,他: "t(11;22)の切断点近傍遺伝子を用いたRT-PCR法によるEwing肉腫およびPNETの診断" 小児がん. 31. 45-48 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 林泰秀,他: "p53遺伝子の点突然変異とN-myc遺伝子の増幅がみられた神経芽腫" 小児がん. 31. 329-332 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Miyashita,T: "Molecular diversion of dominant subclones in vivo and in vitro in Ph-positive ALL." Leukemia. 7. 586-592 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kikuchi,A: "Clinical significance of TAL1 gene alteration in childhood T-cell acute lymphoblastic leukemia and lymphoma." Leukemia. 7. 946-953 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sotomatsu,M: "Establishment of a new human pre-B acute lymphoblastic leukemia cell line(KMO-90)with 1;19 translocation carrying p53 gene alterations." Leukemia. 7. 1615-1620 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Shimizu,K.: "An ets-related gene,ERG,is rearranged in human myeloid leukemia with t(16;21)chromosomal translocation." Proc Natl Acad Sci USA. 90. 10280-10284 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Komuro,H.: "Mutations of the P53 gene are involved in Ewing s sarcomas but not in neuroblastomas." Cancer Res. 53. 5284-5288 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sato,S.: "Effects of herbimycin A and its derivatives on growth and differentiation of Philadelphia chromosome-positive acute lymphoid leukemia cell lines." Leukemia Res. (in press).

    • Related Report
      1993 Annual Research Report

URL: 

Published: 1992-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi