• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification of the ligands of gamma delta T cells

Research Project

Project/Area Number 04454286
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionThe University of Tokyo

Principal Investigator

SHIMADA Shinji  University of Tokyo Branch Hospital, Department of Dermatology, Associate Professor, 医学部(分), 助教授 (10114505)

Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1993: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1992: ¥3,600,000 (Direct Cost: ¥3,600,000)
Keywordsgamma delta T cells / dendritic epidermal T cells / T cell antigen receptor / zeta-chain / Fc receptor / heat shock protein / cytokines / Aly mouse / dendritic epidermal T cell / 3 chain / Fc レセプター / シクロスポリン / intraepithelial lymphocyte / FcεRI / PPD / 樹枝状表皮下細胞 / ケラチノサイト / IL-2 / 熱ショックタンパク / FACS / PCR
Research Abstract

The purpose of this study is to characterize the T cell receptor (TCR) complex of dendritic epidermal T cells (DETC) as a representative of gamma delta T cells and to identify a possible ligand (s) of TCR gamma delta.
TCR is composed of TCR alpha beta or TCR gamma delta associated with CD3 gamma, delta, epsilon and zeta-zeta homodimer. In zeta-chain deficient mice, the degree of TCR expression is significantly reduced. We examined TCR expression of gamma delta T cells which are localized in epithelia and found that the expression of TCR in these mice was not reduced at all. By immunoprecipitation using anti TCR antibodies, gamma chain of Fc receptor for IgE (Fc epsilon R1 gamma=Fc gamma) was utilized instead of zeta chain in DETC and IEL.The signicance of the presense of Fc gamma chain in signal transduction or physiological functions of gamma delta T cells is now under investigation.
With regard to the ligands of gamma delta T cells, the most probable cadidates were heat shock proteins (HSP). We tested several HSPs by fluorescence microscopy, FACS and T cell proliferation assay. DETC,neither freshly semi-purified cells nor cell lines did bind or respond to any HSPs tested. We concluded that the ligands of DETC were not HSPs.
We next examined the cytokine responsiveness of DETC using DETC cell lines (8.27SD and 10.31). We wanted to know whether cytokines produced by keratinocytes are able to affect the functions of DETC.DETC responded to IL-2 or IL-4 equally well but not IL-7 which was thought to be a growth factor of gamma delta T cells. The epidermal cytokines could not influence the response.
In Aly mice, there are no lymphnodes developed. We determined whether DETC were still present or not. In Aly mutant mice, DETC were completely normal in number and the expression of TCR gamma delta. We now investigate whether the functions of DETC are also normal or not.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Shimada S: "T cell receptor expression by dendritic epidermal T cells" Journal of Dermatology. 21. 829-834 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ohno H et al: "Preferential usage of the Fc receptor γ chain in the T cell antigen receptor complex by γ/δ Tcells localized in epithelia" Journal of Expermental Medicine. 179. 365-369 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ota Y et al: "Extrathymic origin of Vγ1-Vδ6 Tcells in the skin" European Journal of immunology. 22. 595-598 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada.S.,: "DETC respond to IL-2 and IL-4 but not IL-7 -Differential effect of cyclosporine A and FK506-" in preparation.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 島田 真路: "上皮内 γδ T細胞の意義" 臨床免疫. 25. 823-828 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 島田 真路: "表皮の免疫担当細胞について" 日本臨床免疫学会会誌. 18. (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 島田 真路: "表皮の免疫生物学" 日本皮膚科学会, 31 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada, S: "T cell receptor expression by dendritic epidermal T cells" Journal of Dermatology. 21. 829-834 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ohno H.et al: "Preferential usage of the Fc receptor gamma chain in the T cell antigen receptor complex by gamma/delta T cells localized in epithelia." Journal of Experimental Medicine. 179. 365-369 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ota Y et al: "Extrathymic origin of V gamma 1-V delta 6 T cells in the skin." European Journal of Immunology. 22. 595-598 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada, S: "Dendritic epidermal T cells respond to IL-2 and IL-4 but not IL-7--Differential effect of cyclosporine A and FK506" (in preparation).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada, S: "The significance of gamma delta T cells in epithelia" Clinical Immunology. 25 (in Japanese). 823-828 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada, S: "The immunocompetent cells in the epidermis." Japanese Journal of Clinical Immunology. (in press) (in Japanese).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada, S: "Immunobiology of the epidermis" Japanese Dermatological Association. (in Japanese). 1-13 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada S: "T cell receptor expression by dendribic epidermal T cells" Journal of Dermatology. 21. 829-832 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 島田眞路: "表皮と免疫" 免疫・Immunology Frontier. 22(印刷中).

    • Related Report
      1994 Annual Research Report
  • [Publications] 島田眞路: "表皮の免疫担当細胞について" 日本臨床免疫学会会誌. 18(印刷中).

    • Related Report
      1994 Annual Research Report
  • [Publications] 島田眞路: "表皮の免疫生物学" 日本皮膚科学会, 31 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ohno H et al: "Preferential usage of the Fc receptor δ chain in the T cell antigen receptor complex by γ/δ T cells localized in epithelia." Journal of Experimental Medicine. 179. 365-369 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 島田眞路: "樹枝状表皮T細胞" 皮膚科の臨床. 35. 1207-1212 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 島田眞路: "上皮内γδT細胞の意義" 臨床免疫. 25. 823-828 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Shimada S et al: "Identification of an eguivalent to murine Thy-1^+ dendritic epidermal cells" Journal of Dermatological Science. 3. 68-71 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ota Y et al: "Extrathymic origin of V_<81>-V_<86> Tcells in the skin" European Journal of Immunology. 22. 595-598 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 島田 眞路: "Thy-1^+樹枝状表皮細胞に関する最近の知見" 日本皮膚科学会雑誌. 102. 1644-1646 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 島田 眞路: "上皮内jgT細胞の意義" 臨床免疫.

    • Related Report
      1992 Annual Research Report

URL: 

Published: 1992-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi