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Basic research for Gd-NCT

Research Project

Project/Area Number 04454296
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 放射線科学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

ONO Koji  Research Reactor Inst., kyoto Univ., professor, 原子炉実験所, 教授 (90122407)

Co-Investigator(Kenkyū-buntansha) MASUNAGA Shinichiro  Research Reactor Inst., Kyoto Univ., assistant professor, 原子炉実験所, 助手 (80238914)
AKABOSHI Mitsuhiko  Research Reactor Inst., Kyoto Univ., associate professor, 原子炉実験所, 助教授 (00027418)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
KeywordsGd-NCT / BNCT / SCCVII / Gd-DTPA / BPA / BPA / GdNCT / SCC7腫瘍細胞 / コロニー形成能 / micronucleus assay
Research Abstract

The current BNCT can not destroy the tumor cells which don't take up boron because alpha particle is too short for strilizing neighbor cells. To break this limitation, we studied the possibility of Gd-NCT using Gd-DTPA.The cell killing effect of Gd-NCT examined in cultured SCCVII tumor cells presented the inferior effect to that of BNCT at all drug cncentrations ; 2,3 and 5 mM.Especially, the cell survival curve at 2 mM has shoulder. In vivo system, Gd-NCT showed maximum effect 2-3 min after the injection of Gd-DTPA, when the effect was asessed by cytokinesis blocked micronucleus assay. In colony formation assay, Gd^<157>N-DTPA(0.6 mu mole/g mouse body weight) yielded the effect being similar to that of B^<10>NPA(750 mg/g, average B^<10>N concentration in tumors = 0.7mu mole/g). We previously elucidated that alpha component of cell survival curve completely appreared as the fraction of cells with normal nuclear division (NNDF) ; without micronucleus appearance nor uneven nuclear division. Since the cell survival curve of SCCVII tumors following Gd-NCT is linear, the homogeneity of B^<10>N distribution at cellular level can be investigated by comparing cell survival curve and NNDF curve. The NNDF curve showed precise agreement with the cell survival curve following Gd-NCT ; -lnNNDF=0.137・T(m) vs.-lnSF=0.131・T(m). In BPA-NCT, however, two curves showed a discrepancy. These findings mean that Gd-DTPA distributed more homogeneously than BPA.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] K.Ono et al.: "Radiosensitization of SCCVII tumors and normal tissues by nicotinamide and carbogen:analysis by micronucleus assay" Radiotherapy and Oncology. 28. 162-167 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Ono et al.: "Middle dose rate irradiation in combination with carbogen inhalation selectively and more markedly increase the responses of SCCVII tumors" Int.J.Radiat.Oncol.Biol.Phys.(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Ono et al.: "Estimation of the initial slope of the cell survival curve after irradiation from micronucleus frequency in cytokinesis-blocked cells" Radiation Research. 138(1)Supplement. S101-S104 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Ono et al.: "Radiosensitization of SCCVII tumors and normal tissues by nicotinamide and carbogen : analysis by micronucleus assay." Radiotherapy and Oncology. 28. 162-167 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Ono et al.: "Middle dose rate irradiation in combination with carbogen inhalation selectively and more markedly increase the responses of SCCVII tumors." Int.J.Radiat.Oncol.Biol.Phys.(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Ono et al.: "Estimation of the initial slope of the cell survival curve after irradiation from micronucleus frequency in cytokinesis-blocked cells." Radiation Research. 138(1)(Supplement). S101-S104 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Ono et al.: "Radiosensitization of SCCVII tumors and normal tissues by nicotinamide and carbogen:analysis by micronucleus assay" Radiotherapy and Oncology. 28. 162-167 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Ono et al.: "Middle dose rate irradiation in combination with carbogen inhalation selectively and more markedly increase the responses of SCCVII tumors" Int.J.Radiat.Oncol.Biol.Phys.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Ono et al.: "Estimation of the initial slope of the cell survival curve after irradiation from micronucleus frequency in cytokinesis-blocked cells" Radiation Research. 138(1)Supplement. S101-S104 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 小野 公二: "放射線抵抗性,難治性の解明" 癌の臨床. 38. 1324-1329 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] S.Masunga,K.Ono and M.Abe: "Potentially lethal damage repair by quiescent cells in murine solid tumors" Int.J.Radiat.Oncol.Biol.Phys.22. 973-978 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Sakagara,K.Ono,T.Saga,K.Akuta,K.Endo J.Konishi and M.Abe: "Hepatocyte response to continuous low dose rate radiation in radioimmunotherapy assessed by micronucleus assay" Int.J.Radiat.Biol.62(4). 443-448 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Akaboshi,K.Kawai,H.Maki,K.Akuta,Y.Ujeno K.Ono and T.Miyahara: "Determination of the target volume of HeLa cells treated with platiνm-195m radiolabeled trans-diamminedichloro-platinum(II)" Nucl.Med.Biol.(1993)

    • Related Report
      1992 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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