| Budget Amount *help |
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1993: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1992: ¥5,000,000 (Direct Cost: ¥5,000,000)
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| Research Abstract |
Perfuluorochemicals (FDA) have two excellent properies, as a good carrier of oxygen and as a imaging agent for tumor and liver. Last year, we examined the radiosensitizing effect (as a oxxgen carrier) of FDA from the changes of phosphate enegy metabolism in transplanted SCCVII tumor using ^<31>P-Magnetic Resonance Spectroscopy (MRS). PCr/Pi showed the most remarkable change among all parameters of ^<31>P-MR spectra obutained from SCC VII tumor and high correlation with tumor growth. From the results of tumor growth delay and ^<31>P-MRS (PCr/Pi) in experimental groups (control, irradiation, oxygen, FDA group), it was concluded that FDA had the radiosen-sitizing effect, and ^<31>P-MRS (PCr/Pi) was useful for monitoring the effects of radiosensitizer, radiation therapy, chemothermia and regrowth after these therapy.
In this year, ^<19>F-MRS (9.4T, GSX400 : JEOL) and ^<19>F-MRI (4.7T, CSI Omega2 : Bruker) of FDA were measured. ^<19>F-peak area in ^<19>F-MR spectra of FDA was detected to 0.02%FDA.^<19>F-peak area in ^<19>F-MR spectra obtained from liver in tumor bearing mouse increased from 1day to 20days after injection of FDA and then decreased. ^<19>F-peak area in ^<19>F-MR spectra obtained from tumor in mouse increased 1day after injection of FDA and decreased according to the growth of tumor. Imaging of FDA was detected to 0.67% of FDA by ^<19>F-MRI.
Two days after injection of FDA (40ml/kg), liver and mouse were able to image by ^<19>F-MRI
FDA might be useful both in radiotherapy as a radiosensitizer and in diagnosis as a tumor imaging agents.
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