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STUDIES ON HYBRID ARTIFICIAL LIVERS IN POINTS OF THE TECHNOLOGY FOR ORGAN RECONSTRUCTION

Research Project

Project/Area Number 04454307
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Agro-economics
Research InstitutionASAHIKAWA MEDICAL COLLEGE

Principal Investigator

KASAI Shinichi  Asahikawa Medical College, Associate Professor, 医学部, 助教授 (40091566)

Co-Investigator(Kenkyū-buntansha) SAWA Masayuki  Asahikawa Medical College, Assistant, 医学部, 助手 (70226059)
KAKISAKA Akitoshi  Asahikawa Medical College, Assistant, 医学部, 助手 (60194677)
YAMAMOTO Tetsu  Asahikawa Medical College, Assistant Professor, 医学部, 講師 (50125415)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordshybrid artificial liver / metabolic support / isolated hepatocytes / microcarrier / culture substrata / hybrid artificial liver / 人工肝臓 / 代謝補助装置 / 肝細胞培養
Research Abstract

Artificial liver and liver transplantation are the only way to replace the deteriorated functions of the damaged livers. However, complete replacement of the original liver functions is not yet possible for artificial livers today, and liver transplantation faces severe scarcity of donor livers.
Recently, artificial livers such as hemodialysis, hemoperfusion, and plasmapheresis have been used clinically to treat patients with liver failure, but the survival rate has not so improved. One of the reasons for this poor survival rate appears to be a lack of metabolic support capacity for the damaged livers. In this study, the development of a hybrid artificial liver was studied in points of the technology for organ reconstruction. Combination of hepatocytes and artificial materials has been attempted to reconstruct the metabolic functions of liver.
1) Natural substrata such as biomatrix of the liver and collagen, and synthetic substrata such as lactose-carrying polystirene were examined in primary culture of isolated hepatocytes, and these were useful for coating artificial materials.
2) Artificial materials such as dextran microcarriers and cellulose multiporous microcarriers were investigated of their availability for immobilizing hepatocytes in a hybrid artificial liver. Hepatocytes immobilized to these microcarriers showed better NH3 and fructose metabolism.
It was therefore suggested that these substrata and carriers were useful for the development of hybrid artificial livers.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Shinichi Kasai: "Rat hepatocytes on a multiporous cellulose microcarrier for a hybrid artificial liver." Artif.Organs Today. 3. 217-225 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shinichi Kasai: "Is the biological artificial liver clinically applicable?" Artifical Organs. 18. 348-354 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 葛西,眞一: "細胞組込み型人工肝臓" 組織培養. 19. 484-488 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 葛西,眞一: "セルローズ由来多孔質マイクロキャリア接着ラット肝細胞の代謝機能の検討" 人工臓器. 22. 159-163 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shinichi Kasai: "Celulose microcarrier for high-density culture of hepatocytes." Transplant.Proc.24. 2933-2934 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shinichi Kasai: "Beneficial effect of hepatocyte transplantation on hepatic failure." Transplant.Proc.24. 2990-2992 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 葛西,眞一: "人工臓器" 日本人工臓器学会, 95 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 葛西,眞一: "人工臓器1993" 阿岸鉄三(中山書店), 322 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Kasai: "Rat hepatocytes on a multiporous cellulose microcarrier for a hybrid artificial liver" Artif.Organs Today. 3. 217-225 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Kasai: "Is the biological artificial liver clinically applicable?" Artificial Organs. 18. 348-354 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Kasai: "Artificial liver supports system using isolated hepatocytes" Tissue Culture. 19. 484-488 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.kasai: "Metabolic activities of multiporous cellurose microcarrier attached hepatocytes" Jap.J.Artif.Organs. 22. 159-163 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Kasai: "Cellulose microcarrier for high-density culture of hepatocytes" Transplant.Proc.24. 2933-2934 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Kasai: "Beneficial effect of hepatocyte transplantation on hepatic failure" Transplant.Proc.24. 2990-2992 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Kasai: Artificial Organs. JSAO, 25-34 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Kasai: Artificial Organs 1993. Agishi Tetsuzo (Nakayama syoten), 29-33 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shinichi Kasai: "Rat hepatocytes on a multiporous cellulose microcarrier for a hybrod artificial liver." Artif.Organs Today. 3. 217-225 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shinichi Kasai: "Is the biological artificial liver clinically applicable?" Artifcial Organs. 18. 348-354 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 葛西眞一: "FOCUS消化器(2)腹部救急医療" 中山書店, 203 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 葛西眞一: "人工臓器" 日本人工臓器学会, 95 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 葛西眞一: "細胞組込み型人工肝臓" 組織培養. 19. 484-488 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Bojian Jiang: "Beneficial effect of-HSS on the survival of intrasplenically transplanted hepatocytes." Cell Transplantation. 2. 325-329 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] S.Hirai: "Encapsulated hepatocytes transplantation for the treatment of D-Gal-induced acute hepatic failure in rats." Eur.Surg.Res.25. 193-202 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 小野寺一彦: "^<99>Tc-PMTシンチによるラット脾内肝細胞の生着評価" 今日の移植. 6. 191-195 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 葛西眞一: "人工臓器1993" 阿岸鉄三(中山書店), 322 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 葛西 眞一: "Hybrid Artificial Liverの研究 ーセルロース系マイクロキャリアへの肝細胞接着動態ー" 人工臓器. 21. 1055-1059 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] S.Kasai: "Cellulose Microcarrier for High-Density Culture of Hepatocytes." Transplantation Proceedings. 24. 2933-2934 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] S.Kasai: "Beneficial Effect of Hepatocyte Transplantation on Hepatic Failure in Rats." Transplantation Proceedings. 24. 2990-2992 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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