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A basic research on muscle fiber restoration in transplanted muscle.

Research Project

Project/Area Number 04454311
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General surgery
Research InstitutionUniversity of Tokyo

Principal Investigator

YAMADA Atsushi  Univ.of Tokyo, Faculty of Medicine, Associate Professor, 医学部・(病), 助教授 (60107662)

Co-Investigator(Kenkyū-buntansha) YOSHIMURA Kotaro  Univ.of Tokyo, Faculty of Medicine, Associate, 医学部・(病), 助手 (60210762)
ASATO Hirotaka  Univ.of Tokyo, Faculty of Medicine, Associate, 医学部・(病), 助手 (20222581)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1993: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1992: ¥6,200,000 (Direct Cost: ¥6,200,000)
Keywordsmuscle transfer / muscle regeneration / myosin / isozyme / microsurgery / muscle fiber type / マイクロサージャリー / 脱神経 / 再神経支配 / 交差(異)神経支配 / ラット / ミオシンアイソザイム
Research Abstract

We created a new rat model of muscle transplantation to the face in order to examine muscles transplanted with microneurovascular anastomoses. Using this model enabled comparison of restoration of transplanted muscles electrophysiologically and histologically with that of denervated and self-innervated muscles. Adult male rats were utilized in this study. At 1, 2, 3, 4, 8, 12 and 16 postoperative weeks muscle reinnervation in 5 rats was examined by EMG, followed by sacrificing the rats to obtain histological specimens. The evoked potential of the reinnervated muscle was recorded simultaneously. Transverse sections of the transplanted muscles stained by H-E and the histochemical method to demonstrate the myosin ATPase activity were examined under the light microscope. Pyrophosphate polyacrylamide gel electrophoresis of myosin crudely extracted from the specimens was performed. Similarly were examined both denervated gracilis muscles and self-innervated muscles.
The EMG and histological r … More esults of our study revealed that the difference in restoration between transplanted muscle and self-innervated muscle was remarkable, indicating the prominent effects of several problems as ischemia, tenotomy, surgical invasion. The histochemical results of this experiment cannot fully be explained by the neural regulatory hypothesis, while it can be explained by Hoh et al.'s hypothesis, indicating that the muscle fibers may be intrinsically heterogenous and may be "plastic" within the constraints. The 'undifferentiated' type 2c fiber, observed during fetal and neonatal development or regeneration, is thought to contain EM or NM or both. The 'transitional' type 2c fiber, which is expressed during transformation of muscle fiber type caused by denervation, electrical stimulation, foreign-reinnervation, and so on, was, however, found to contain mixtures of SM and FM.Therefore, two kinds of type 2c fiber may exists, though they are phenotypically identical each other with regard to myosin ATPase activity. The appearance of EM or NM during denervation period may indicate possibility of dediffrentiation of myosin isoforms. Less

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 吉村浩太郎: "神経血管柄付き遊離移植筋に関する実験的研究-第1報-" 日本形成外科学会会誌. 13. 239-250 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 吉村浩太郎: "神経血管柄付き遊離移植筋に関する実験的研究-第2報-" 日本形成外科学会会誌. 13. 359-370 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 吉村浩太郎: "神経血管柄付き遊離移植筋に関する実験的研究-第3報-" 日本形成外科学会会誌. 13. 675-684 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 吉村浩太郎: "神経血管柄付き遊離移植筋に関する実験的研究-第4報-" 日本形成外科学会会誌. 13. 685-692 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kotaro YOSHIMURA, Takao KAWASHIMA, Atsushi YAMADA and Kiyonori HARII: "An Experimental Study on Muscle Transplanted with Microneurovascular Anastomoses Part 1 : A rat experimental model ; Electromyographical and histological analyzes on restoration of transplanted muscles, compared with denervated and self-innervated muscles" J.of Japan Society of Plastic and Reconstructive Surgery. 13. 239-250 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kotaro YOSHIMURA, Takao KAWASHIMA, Atsushi YAMADA and Kiyonori HARII: "An Experimental Study on Muscle Transplanted with Microneurovascular Anastomoses Part 2 : Histochemical analyzes of transplanted muscle fibers, compared with denervated and self-innervated muscle fibers" J.of Japan Society of Plastic and Reconstructive Surgery. 13. 359-370 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kotaro YOSHIMURA, Takao KAWASHIMA, Akihiro KAWATO, Celina Mitiko Hatanaka, Atsushi YAMADA and Kiyonori HARII: "An Experimental Study on Muscle Transplanted with Microneurovascular Anastomoses Part 3 : Biological analyzes of myosin isoforms of transplanted muscle fibers by using a pyrophosphate electrophoresis" J.of Japan Society of Plastic and Reconstructive Surgery. 13. 675-684 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kotaro YOSHIMURA, Takao KAWASHIMA, Akihiro KAWATO, Atsushi YAMADA and Kiyonori HARII: "An Experimental Study on Muscle Transplanted with Microneurovascular Anastomoses Part 4 : Biological analyzes of myosin isoforms in denervated and self-innervated muscle fibers using a pyrophosphate electrophoresis" J.of Japan Society of Plastic and Reconstructive Surgery. 13. 685-692 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 吉村浩太郎: "神経血管柄付き遊離移植筋に関する実験的研究-第1報-" 日本形成外科学会会誌. 13. 239-250 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 吉村浩太郎: "神経血管柄付き遊離移植筋に関する実験的研究-第2報-" 日本形成外科学会会誌. 13. 359-370 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 吉村浩太郎: "神経血管柄付き遊離移植筋に関する実験的研究-第3報-" 日本形成外科学会会誌. 13. 675-684 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 吉村浩太郎: "神経血管柄付き遊離移植筋に関する実験的研究-第4報-" 日本形成外科学会会誌. 13. 685-692 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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