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Studies on pathophysiology after hepatectomy for dogs with obstructive jaudice under occlusion of hepatic blood inflow

Research Project

Project/Area Number 04454329
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionMIE University

Principal Investigator

MIZUMOTO Ryuji  Mie University, Faculty of Medicine, Professor, 医学部, 教授 (00025561)

Co-Investigator(Kenkyū-buntansha) OGURA Yoshifumi  Mie University, Faculty of Medicine, Lecturer, 医学部, 講師 (00126963)
NOGUCHI Takashi  Mie University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (40144258)
KAWARADA Yoshifumi  Mie University, College of Medical Sciences, Professor, 医療技術短期大学部, 教授 (40024814)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥6,400,000 (Direct Cost: ¥6,400,000)
KeywordsIschemia-reperfusion injury / Obstructive jaundice / 40% hepatectomy / Free radical / Free radical scavenger / Endotoxin / Superoxide dismutase / Active bypass / 黄疸遷延 / 網内系機能 / 肝微細構造
Research Abstract

Obstructive jaundice was produced in adult mongrel dogs by cholecystectomy and ligation and cutting of the distal common bile duct. The pathophysiology of ischemia-reperfusion injury on remnant liver after hepatectomy and the occlusion of hepatic blood inflow was evaluated in dogs with obstructive jaundice.
1.When 40% hepatectomy under 10-minute occlusion of both hepatic artery and portal vein was performed for dogs with obstructive jaundice at 2 weeks after ligation of the common bile duct and chole-cystectomy, the content of lipid peroxide increased significantly in the remnant liver with reduction of SOD activity, while xanthine oxidase activity did not change. The levels of endotoxin and beta-NAH in peripheral blood had significantly increased, and phagocytic index had markedly decreased after 3 hours. Survival rate at 7 days was only 23.1% and many dogs died of liver failure.
2.Effects of free radical scavengers : When Coenzyme Q_<10>(CoQ_<10>) or stirene-co-maleic acid superoxide d … More ismutase(SM-SOD) was administered before ischemia and reflow, the development of the above phenomenon in the remnant liver was prevented significantly and the survival rate at 7days was improved remarkably to 76.9% and 69.2%. The endotoxin levels in the portal venous blood were increased significantly after reflow of the hepatic blood inflow, regardless of the administration of CoQ_<10> or SM-SOD.Whereas, the levels in the peripheral blood were markedly reduced by these drugs.
3.Effects of active bypass of the portal vein to inferior vena cava : When the hepatic blood inflow was occluded for 10 minutes with active bypass of the portal vein to inferior vena cava, the levels of endotoxin in both the portal venous blood and the peripheral blood did not change, and the remnant liver injury was milder. However, when the hepatic blood inflow was occuluded for 20 minutes with active bypass, the content of lipid peroxide increased significantly in the remnant liver with reduction of SOD activity, while the levels of endotoxin in both the portal venous blood and the peripheral blood remained the same. The level beta-NAH in peripheral blood increased significantly and the phagocytic index decreased markedly after 3 hours. Survival rate at 7 days was only 33.3% and many dogs died of liver failure. Less

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] 水本 龍二: "臨床医学の展望-肝.胆道.膵外科" 日本医事新報. 3380. 15-21 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 水本 龍二: "胆道癌の治療成績-進行癌に対する拡大手術を中心として(アンケート集計結果から)" 胆と膵. 11. 869-882 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ryuji Mizumoto: "Definition and diagnosis early cancer of the biliary tract" Hepato-Gastroenterol.40. 69-77 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 川原田 嘉文: "肝予備能検査" 外科治療. 66. 559-563 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 野口 孝: "胆管細胞癌をめぐる諸問題-肝内胆管癌.治療" 肝胆膵. 24. 249-259 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 小倉 嘉文: "胆道癌に対する拡大手術と縮小手術" 消火器外科. 16. 1549-1559 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 水本 龍二: "胆道癌:診断と治療の進歩「消化器病セミナー」" へるす出版, 18 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 水本 龍二: "「図説臨床[癌]シリーズNo3.肝癌」胆管細胞の臨床" メジカルビュー社, 8 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ryuji MIZUMOTO: "The view of clinical medicine-surgery of the liver, biliay tract and pancreas" Japan Medical J(in Japanese). 3380. 15-21 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ryuji MIZUMOTO: "Cooperative survey of surgical treatment for carcinoma of biliary tract in Japan" TAN-to-SUI(in Japanese). 11. 869-882 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ryuji MIZUMOTO: "Definition and diagnosis early cancer of the biliary tract" Hepato-gastroenterol. 40. 69-77 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yoshifumi KAWARADA: "Estimation of hepatic functional reserve" Surgical Therapy(in Japanese). 33. 559-563 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takashi NOGUCHI: "Surgical treatment for cholangiocellular carcinoma, with special reference to clinicopathological study and prognosis after liver resection" KAN-TAN-SUI(in Japanese). 24. 249-259 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yoshifumi OGURA: "Choice of extended or limited operation for carcinoma of biliary tract" Gastroenterol Surg. 16. 1549-1559 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ryuji MIZUMOTO: "History and recent trends of the treatment for carcinoma of the biliary tract" Herusu-shuppan(in Japanese). 18. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ryuji MIZUMOTO: "'Progress in cancer series No3. Liver cancer' Clinical findings in cancer of intrahepatic bile duct" Medical-view(in Japanese). 8. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 水本龍二: "臨床医学の展望-肝・胆道・膵外科" 日本医事新報. 3380. 15-21 (1989)

    • Related Report
      1993 Annual Research Report
  • [Publications] 水本龍二: "胆道癌の治療成績-進行癌に対する拡大手術を中心として(アンケート集計結果から)" 胆と膵. 11. 869-882 (1990)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ryuji Mizumoto: "Definition and diagnosis early cancer of the biliary tract" Hepato-Gastroenterol.40. 69-77 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 川原田嘉文: "肝予備能検査" 外科治療. 66. 559-563 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 野口孝: "胆管細胞癌をめぐる諸問題-肝内胆管癌.治療" 肝胆膵. 24. 249-259 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 小倉嘉文: "胆道癌に対する拡大手術と縮小手術" 消化器外科. 16. 1549-1559 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 水本龍二: "胆道癌:診断と治療の進歩「消化器病セミナー」" へるす出版, 18 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 水本龍二: "「図説臨床[癌]シリーズNo3.肝癌」胆管細胞癌の臨床" メジカルビュー社, 8 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ryuji Mizumoto: "Surgical anatomy of the hepatic hilum with special reference to the caudate lobe" World J Surg.12. 2-10 (1989)

    • Related Report
      1992 Annual Research Report
  • [Publications] 水本 龍二: "臨床医学の展望ー肝、胆道、膵外科" 日本医事新報. 3380. 15-21 (1989)

    • Related Report
      1992 Annual Research Report
  • [Publications] 水本 龍二: "胆道癌の治療成績ー進行癌に対する拡大手術を中心として(アンケート集計結果から)" 胆と膵. 11. 869-882 (1990)

    • Related Report
      1992 Annual Research Report
  • [Publications] 川原田 嘉文: "肝予備能検査" 外科治療. 66. 559-563 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 野口 孝: "胆管細胞癌をめぐる諸問題ー肝内胆管癌、治療" 肝胆膵. 24. 249-259 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 小倉 嘉文: "胆道癌" 肝胆膵. 25. 739-747 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 水本 龍二: "胆道癌:診断と治療の進歩「消化器病セミナー」" へるす出版, 18 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 水本 龍二: "「図説臨床[癌]シリーズNo3.肝癌」胆菅細胞癌の臨床" メジカルビュー社, 8 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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